We narrowed to 329 results for: AGA
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TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m.... gather more feedback, and start the cycle over again to refine the solution, or to come up with a new...
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Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog PostPublishedSept. 15, 2020, 1:15 p.m....resistance marker in the backbone. A selection on agar containing ampicillin will yield bacteria that have... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m....for variants that infect the CNS while selecting against those that also infect the liver. Fig... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m....Additionally, align a custom nucleotide sequence against a given sequence using BLAST2. See this blog post... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....on our Viral Service (and then we crunched them again) to find the most requested lentivirus and AAV of... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....to methylation if the enzyme's recognition site (TCTAGA) is preceded by GA or followed by TC. As shown ... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m.... rules that I try to follow in my own research. Again, some of this may not apply or may not work for ... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....proteins inside a cell have a commercial antibody against them. There’s also a question about how specific... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs targeted... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....like those pioneered by Ewen Mullins of Ireland’s Teagasc. Plant biotechnology is full of potential, but... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships grow naturally... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....in r/molecularbiology. Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....from a complex mix using affinity chromatography against an amino acid sequence, or tag, on the protein.... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m....found (typically RNA that the RNAi is designed against), RISC can repress translation and/or degrade target... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....constantly communicating with a tumor, proving once again that a tumor is always interacting with its surroundings... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....here at our new location to grow. We are hiring again and getting ready to embark on new exciting projects... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....full-length protein that you want to generate antibodies against, or a unique peptide sequence derived from this... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....localization of the hM4Di, we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....check the fragments size of your digestion on an agarose gel. If the digestion does not provide the fragments... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....you!) Amount of information and level of detail Again, this is an obvious one on the surface, but it’s...