We narrowed to 332 results for: AGA

1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...mCherry expression using FACs sorting, confirming yet again that sgRNA structure and genomic recognition sites...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...editing speed dictates which phage variants can propagate faster than the rate of dilution (passage from...

3. Hot Plasmids - May 2022

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   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...for variants that infect the CNS while selecting against those that also infect the liver.    Fig...

4. Antibodies 101: Isotypes

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   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies. They...

5. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ....1016/j.neuron.2016.01.040 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of ...

6. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   Published

   Dec. 11, 2018, 12:46 p.m.

   ...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences...

7. A Quick Guide to a Career in Software Product Management

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   Blog Post

   Published

   Nov. 24, 2020, 2:15 p.m.

   ... gather more feedback, and start the cycle over again to refine the solution, or to come up with a new...

8. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...to methylation if the enzyme's recognition site (TCTAGA) is preceded by GA or followed by TC. As shown ...

9. How to Keep a Lab Notebook for Bioinformatic Analyses

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   Blog Post

   Published

   June 2, 2016, 2:30 p.m.

   ... rules that I try to follow in my own research. Again, some of this may not apply or may not work for ...

10. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...proteins inside a cell have a commercial antibody against them. There’s also a question about how specific...

11. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs targeted...

12. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full of potential, but...

13. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post

    Published

    Feb. 4, 2014, 3:50 p.m.

    ...really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships grow naturally...

14. Addgene: The First Twenty Years

    Type

    Blog Post

    Published

    Dec. 19, 2024, 2:15 p.m.

    ...in r/molecularbiology.   Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production...

15. To Each HIS Own

    Type

    Blog Post

    Published

    March 2, 2023, 2:15 p.m.

    ...from a complex mix using affinity chromatography against an amino acid sequence, or tag, on the protein....

16. All in a Twist: dsRNA

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    Blog Post

    Published

    Feb. 2, 2023, 2:15 p.m.

    ...found (typically RNA that the RNAi is designed against), RISC can repress translation and/or degrade target...

17. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ... a kinases in a body part of choice. Zhou et al again used the Raf-MEK-ERK signaling pathway to demonstrate...

18. Summer SciComm Series: Cognitive Load

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    Blog Post

    Published

    June 16, 2022, 1:15 p.m.

    ...you!)  Amount of information and level of detail Again, this is an obvious one on the surface, but it’s...

19. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...full-length protein that you want to generate antibodies against, or a unique peptide sequence derived from this...

20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...localization of the hM4Di, we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold...