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TypeBlog PostPublishedDec. 7, 2015, 6 p.m....2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array ...vivo gene delivery, but have limited packaging capacity making them difficult to use with CRISPR as the...
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Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....for different ions. Right now, we can modulate electricity. If you could selectively let in calcium or some...Alerts box. References: Independent Optical Excitation of Distinct Neural Populations. Nathan Klapoetke... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....Understanding) Writing Communication Publicity Consulting internships Communication...Blogging Writing Communication Networking Publicity Business competitions Leadership ... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....2019), “this localization should improve the specificity of influence measurements by reducing photostimulation...continue to grow this collection. Of note, we are excited to release a Flp-dependent Cre AAV in AAV9 and ... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m.... the Addgene-Seeding Labs plasmid grant we are excited to introduce the 5 awardees and their research.... out some of our recent endeavors here. We are excited to see how all of the Addgene-Seeding Labs Plasmid... -
Trading Spaces - Visiting Scientist Edition
TypeBlog PostPublishedAug. 22, 2023, 1 p.m....while experiencing a new city or maybe even a new country can also be exciting! Identifying a host institute... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....so it is an invaluable tool to be used in this capacity. Similar to the Human TFome Library, the MORF ...identification. While the Zhang lab controlled their multiplicity of infection so that a single TF was delivered... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....Center for Open Science, Dr. Mellor worked with citizen scientists to design and implement authentic scientific...His dissertation and research background is in citizen science and behavioral ecology of cichlid fishes... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....starting our viral vector service in 2016...we’re excited to help scientists share their reagents with the... for supporting scientific sharing. We’re very excited for more reagent sharing projects in the years ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....determine brightness are the extent to which the excitation light is absorbed and the efficiency by which...account, including the specs of your microscope (excitation wavelength, available emission filters, and detector... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....for our newsletter and a free plasmid) were very excited about our new viral services. If you’re not familiar...educational content on the blog. Overall, there are many exciting things going on in the world of neuroscience and... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and would like to get it working in microglia...development of more and smaller CRISPR tools is an exciting area for future AAV application to epigenetic ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....possible. This advancement could be especially exciting for scientists working to visualize multiprotein...tools to include nanobodies. At Addgene, we are excited to be part of this initiative and to continue to... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades. So let’s break ...engineered receptors to interact with small molecules to elicit a response in a certain cell type. Chemogenetics... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....internships share a common set of criteria such as citizenship status, academic year, and grades that serve ...applying to several opportunities. In an age of scarcity, the rational human being will tend to clinch ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....perform retrograde tracing, but often at the cost of toxicity, low expression, or poor scalability. Scientists...neurons within their natural environment, all while reciting their favorite slogan, “look how cool the brain... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....active throughout the cell cycle and has a higher capacity for repair, as there is no requirement for a repair...RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....the development and metastasis of tumors through inciting inflammation. Speaking of inflammation, a noteworthy... promote microenvironment’s malignancy but also incite different organs to become malignant. Exosomes ... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...protein to create three distinct colors. The specificity of these orthologs is key: since each ortholog... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....big plans for our future. And while we’ve been excited to share them with you, we realized our 20th anniversary... millionth item, and had a cumulative 120,000 citations of individual items in our repository, a tribute...