We narrowed to 573 results for: RON

1. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ...for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins. In: Methods in Molecular Biology...

2. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ...brought issues in reproducible research to the forefront in the so-called “reproducibility crisis.” Results...

3. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ...more open way of communicating in science brings stronger networking across laboratory and disciplinary ...

4. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...individually (~20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing...

5. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...awkwardness of getting the right antibody with the wrong everything else.      References and Resources ...

6. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   Published

   Feb. 2, 2021, 2:15 p.m.

   ...opportunity for themselves. This is ultimately the wrong approach. It is very likely you know individuals...

7. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...both small and potentially less likely to elicit a strong immune response, factors that may make it especially...

8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...abilities to turn genes on and off,” he said. “Some are strong and some more moderate; there is an allelic series...

9. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   Published

   Aug. 7, 2014, 1:58 p.m.

   ... At A Scientific Conference Dinner - by Julio Peironcely on the Thesis Whisperer "Not Networking - Building...

10. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for 50+...

11. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific...

12. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...segment of the government, so it was a really good environment to be looking for a job.” Thanks to Susan for...

13. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...many FRET-opsin GEVIs. With an increase in the neuron’s membrane potential, Ace absorbance increases and...

14. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ... resources, and, on the rare occasion things go wrong, replace them for free. Plasmids ship out 2-3 days...

15. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...practices for reproducibility: data management plans, electronic lab notebooks, wet lab protocols, reagent sharing...

16. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ...the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and...

17. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...10kD synthetic binding proteins which use the Fibronectin type III domain as their scaffolding instead ...

18. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...quality. But even journal-based peer review can be prone to biases or errors, with a lack of transparency...

19. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ... the correct plasmid. There’s a lot that can go wrong! With all the steps in the cloning process, there...

20. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...repetitive regions (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors...

21. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...multimers using class-IIS restriction enzymes. Lee JH, Skowron PM, Rutkowska SM, Hong SS, Kim SC. Genet Anal.1996...

22. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...organisation like Building with Biology. Local environmental groups, charities and NGOs are often looking...

23. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...tags (e.g. FLAG and STREP) and auxin-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids...

24. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... For plasmids with highly repetitive sequences (prone to recombination in bacteria) such as lentiviral...

25. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ...manipulation to produce and edit plasmids. These are prone to error — both human and biological. Even the most...

26. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...Gate Assembly with NEB’s DAD Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ...

27. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...transducing a cell line of interest a lot can go wrong. We hope that we have provided you with a few ideas...

28. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...combinations and pick the conditions that give the strongest biotinylation signal, as measured via Western ...

29. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...activation can occur through fusing dCas9 with VP64, a strong transcriptional activation domain. Guided by dCas9...

30. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    .... Either way, just let the interviewers know up front how you plan to take questions.  Relax!  You wouldn...

31. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    Published

    Jan. 9, 2020, 1:45 p.m.

    ... contain a short summary of the topic and a chronological list of highly cited papers that contain your...

32. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...editing to treat sickle cell disease-A brief update. Front Med (Lausanne). 2023 Jan 9;9:1065377. doi: 10.3389...

33. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...cultures do, and tired researchers are accident-prone researchers. Note that safety policies will vary...

34. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    Published

    Aug. 6, 2019, 12:55 p.m.

    ...control mice will have been raised in the same environment and will harbor similar epigenetic modifications...

35. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 3, 2022, 1:15 p.m.

    ...some older split-GFP constructs or certain cell environments). Also consider that a protein fusion may disrupt...

36. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ... S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann ...

37. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...intracellular injections into the cytoplasm or pronucleus of the zygote which is both technically challenging...

38. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ... by experts in their respective fields: David Cameron, PhD - Director of Media Communications, Harvard...

39. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...cognitive disabilities, problem behaviors, and a chronic feeling of hunger that can lead to excessive eating...

40. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo...