We narrowed to 570 results for: RON
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TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....AAV, but also form secondary structures that are prone to deletion from the transfer plasmid. The good ...
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Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....ligation efficiency and identification of mismatch-prone pairings. Using these 4 bp overhang observations... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....studying biosignature preservation in Mars analogue environments. Alejandro (Alex) Chavez is a member of ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m.... circulatory and lymph systems are not ideal environments for many cell types. From proteins to pathways... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....et al. "DNA-guided genome editing using the Natronobacterium gregoryi Argonaute." Nature biotechnology ... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....improve plasmid stability and yield. If a plasmid is prone to recombination Use a strain optimized for plasmids... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m.... with a good public school system that has made strong efforts in the last 5 years to incorporate more... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m.... Ashley Waldron... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....Bacteria Concentrations in Indoor and Outdoor Air. Environmental Science & Technology Letters, 2(4), 84. https... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....Agrobacterium vectors for plant synthetic biology’, Frontiers in Plant Science, 4. doi: 10.3389/fpls.2013.00339... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....CRISPRko libraries are highly effective at identifying strong hits in genetic screens, they do have several limitations... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....product, then you know there could be something wrong with the PCR setup and/or the primer design. ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m.... proteins at once. However, as Y2H screens are prone to both false positive and false negatives, these... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....collections. Many companies, such as Thermo Fisher and Micronic, provide 2D barcoded tubes. 1D barcodes are better... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....Japanese science. I really miss the intellectual environment in the US in this regard. In addition, not many... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....et al. show that genome-wide CRISPR screens are prone to some of the same flaws as RNAi screens, such ... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m.... people and make you work in a collaborative environment. Think of student groups, PostDoc associations... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m.... mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....our pipeline on a local computer or in a cloud environment. Given the throughput necessary to keep up with... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....most prominent idea, and instead building an environment where everyone has their ideas heard. So how... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....References and resources References: Björck L, Kronvall G (1984) Purification and some properties of streptococcal... -
How to Be an Excellent Trainee
TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....what you don’t know – i.e., if they use a term or acronym you don’t know, or if they assume you have done... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....protocols therefore differ from library to library. We strongly recommend following the protocols provided by ... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....Synergistic Activation Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....slow-growing cells, particularly mammalian cells like neurons, may not replicate during the course of your experiment... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....we launched the Addgene YouTube channel to add a strong visual component to our educational resources. ... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....Repetitive sequences Restriction sites Sequences prone to create secondary structures in RNA transcripts... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....think this is represented by factorials. In environmental science and ecology, this can be represented... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m....be exposed to asbestos. However, as the U.S. Environmental Protection Agency has noted, there is no safe... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m....110.18 (2013). 7306-11. Pubmed PMID: 23589850. 9. Cronin, J., Zhang, X.Y., Reiser, J. “Altering the tropism... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....viable? Or cytotoxic? How and where did things go wrong? The use of proper control plasmids would address... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....in many FRET experiments. In addition, other environmental factors, such as pH or autofluorescence background... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m.... Ashley Waldron... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....method is single strand annealing (SSA), an error prone mechanism that notably does not require a donor ... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....referred to as sequence DNA as it synthesized by electronically recording fluorescence activity as fluorescently... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389...