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We narrowed to 419 results for: RAP

Showing: 21 - 40 of 419 results

  1. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...scientist and photographer based at the University of Queensland, whose outstanding marine photography has attracted...” Daniel went on to discuss his work: “Macro photography has been my passion for a while - a tool to expand...life cycle of the creature they deal with.” So photography can be used as a way to better understand and...scientist and non-scientist alike. “The other thing photography has shown to me - it requires an awful amount...data, publish in time, defend the thesis, etc. Photography is critically important in many fields of biology... can be found on his website http://www.microworldsphotography.com/. Another artist who spent his life...acclaim - including being featured in the National Geographic and Smithsonian Magazine, where the editor stated...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja (2017). PubMed...being made to make them even more useful for therapeutic purposes. The University of Pennsylvania and ...These vectors can be used to improve targeted therapies for the eye, skeletal muscles, and the central...Cas9 but have the potential to deliver other therapeutic molecules such as microRNAs (miRNAs), short hairpin...insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007): 1740-1743. PubMed PMID: 17882145....tropism in mice after systemic injection." Molecular Therapy 16.6 (2008): 1073-1080. PubMed PMID: 18414476. ...anterograde and retrograde axonal transport." Human gene therapy 25.8 (2014): 705-720. PubMed PMID: 24694006. PubMed...

  3. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...response. T cell epitopes are found in either the therapeutic antibody itself or as impurities in the formulation... algorithm to evaluate the immunogenicity of therapeutics in silico. These algorithms are trained on vast...algorithms fail to capture. In vitro evaluation of ‌therapeutic immunogenicity is typically performed using traditional...In-vitro models for immunogenicity prediction of therapeutic antibodies. Used under CC BY fromGroell et.al... et.al, 2018). Treating these cells with the therapeutic antibody can help us assess the effects of the...researchers to develop safer and more effective mAb therapeutics. The in silico method of immunogenicity assessment...

  4. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...Using rAAV6. Molecular Therapy 19:36–45 . https://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella...sequence derived from the filamentous phage F1 (Trapani et al., 2014). Drawbacks: The choice of homology...need to be optimized for each unique application (Trapani et al., 2015).  Expression of smaller fragments... of Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140...Capacity in a Transgene-independent Manner. Molecular Therapy 16:124–130 . https://doi.org/10.1038/sj.mt.6300322...Reassembly of Adeno-associated Virus. Molecular Therapy 18:6–8 . https://doi.org/10.1038/mt.2009.280 Hirsch...DNA-PKcs-independent, Rad51C-dependent Repair Pathway. Molecular Therapy 21:2205–2216 . https://doi.org/10.1038/mt.2013.184...

  5. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...immunology, cancer-immune cell interactions, cancer immunotherapy, as well as new approaches to reprogramming ...reprogramming tumor-associated inflammation for therapeutic benefit ”as stated by the organizers. My overall impression... discussed the effects of starvation on cancer therapy. Findings in previous animal studies suggested ...immunosuppressive reaction in order to improve therapeutic outcomes.  If you are studying inflammation and...to help rid us of cancer in combination with chemotherapy. To dive deeper into this field read our recent...doctoral candidate at Binghamton University! Cancer therapeutics The so called field of Immuno-Oncology, a sizzling... hot topic, includes studies on novel cancer therapeutics such as  (Chimeric Antigen Receptors) CAR T ...

  6. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...attractive vectors for research applications and gene therapy: they can be produced at high titers, can accommodate...the Adv genome’s utility in research and its therapeutic potential. Note: constructs discussed are based...Potential applications for gene and cell-based therapies in the new era of personalized medicine (2017)...Gutless AdV have generated high interest for gene therapy due their increased cloning capacity (up to 36 ...20 additional days at which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The ...References 1. Luo, Jinyong, et al. "A protocol for rapid generation of recombinant adenoviruses using the... the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and production of high-capacity...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ... vectors in cancer immunotherapy. Oldham RA, Berinstein EM, Medin JA. Immunotherapy. 2015;7(3):271-84.... and they’ve also had success in clinical gene therapy applications. Read on to learn more about the current...2nd generation lentiviral packaging system The graphic below shows how the lentiviral genome was condensed...interested in developing these vectors for gene therapy. In 2003, VIRxSYS began the first clinical trial...of 2013, 65 patients had been treated with this therapy, which lowered viral load and did not cause severe...Lentiviral vectors are promising agents for cancer immunotherapy, but this research is still in the pre-clinical...These safer vectors have become standard in gene therapy. Researchers have also developed integrase-deficient...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...gene therapy have also been in the limelight. The FDA approved the first oncolytic viral therapy in 2015... science research all the way to clinical gene therapy. But how can you use these amazing tools in your...libraries makes them the ideal vector for the job. Therapeutic applications Vaccine development Attenuated and...being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low immunogenicity, and...for human patients. Multiple viral-based gene therapies have already been approved by the FDA, and this...you are interested in basic science or clinical therapy—viruses could further your efforts. And Addgene... et al. Viral vector platforms within the gene therapy landscape. Sig Transduct Target Ther 6, 53 (2021...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...inverted terminal repeat sequences. Human Gene Therapy Methods, 23(1), Article 1. https://doi.org/10.1089... Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation of gene expression...can limit production of recombinant AAV-2. Gene Therapy, 6(7), Article 7. https://doi.org/10.1038/sj.gt...Inactivation of AAV Serotype 1 Vectors. Human Gene Therapy Methods, 28(1), Article 1. https://doi.org/10.1089...Vectors by Oxidant-Based Disinfectants. Human Gene Therapy, 32(13–14), Article 13–14. https://doi.org/10.1089...genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089...type 2 Reference Standard Material. Human Gene Therapy, 21(10), Article 10. https://doi.org/10.1089/hum...

  10. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...versus the response and use that information to extrapolate the concentration of an unknown sample. For example...standard curve? To create a standard curve, you graph the measured response of the serially diluted standard...absorbance is plotted on the y-axis. With the help of graphing software, you then generate a trendline for the...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample based...sample is determined by using the trendline to extrapolate its x value, 0.963 mg/mL, from its y value, 0.651...the quality of a standard curve scientists use graphing software to calculate the data’s coefficient of..., manufacturers often recommend using advanced graphing software designed to plot complex trendlines. ...

  11. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...Some things cannot be photographed or need text to properly contextualize. Graphics/cartoons (no words...be a hard sell!) Digital platforms often have demographic data of their users readily accessible via web...have a solid understanding of their audience demographics. Do your research so you can go to where your...depending on factors like socioeconomic status and geographic location. For example, not all social media platforms... biology are often more suited for cartoons or graphics for visuals. However, it’s much more challenging...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...may need text for audience interpretation. Infographics Low Combines both words and images; especially...

  12. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness and ...’s size makes it difficult to deliver via gene therapy, so researchers have set their sights on another...work with dystrophin exon skipping? For such a therapy to be successful, multiple requirements must be...minimal risk of off-target editing. In order for the therapy to persist over time, stem cell editing would be...in DMD, and it’s estimated that exon skipping therapies would be applicable to 80% of DMD patients. ...characterizes additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause...represent a great step forward for CRISPR gene therapy, it’s important to realize that DMD is a simpler...

  13. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...discovery/development of genetic targets and RNA therapies. [6]. In 2014, we wrote a blog post on CRISPR’...researchers are now using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting... may not be the most likely candidate for gene therapy, however, because the disease affects multiple ...suggest it might work well enough to consider as a therapeutic.” There are many fundamental questions about ...mutations when using this technology. When CRISPR therapies are being given in vivo, it is difficult to use... References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology.  https://doi....1389201021666200621161610 4. Uddin et al. 2020.  “CRISPR Gene Therapy: Applications, Limitations, and Implications for...

  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), 1–41. https://doi.org... Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169...Carroll, S. J. (2019). Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in... in the rat spinal cord following injury. Gene Therapy, 26(5), Article 5. https://doi.org/10.1038/s41434...Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic Acids, 8, 184–197. https://doi.org/10.1016...corticospinal tract: Comparison of four promoters. Gene Therapy, 28(1–2), 56–74. https://doi.org/10.1038/s41434...

  15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...development of toolkits such as the SapTrap CRISPR/Cas Toolkit and SapTrap-SEC kit for C. elegans genome engineering...unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the need for ...rescued progeny, which makes screening extremely rapid. This stringent selection allowed me to optimize...built on this work, discovering that melting and rapidly cooling dsDNA repair templates dramatically boosted...repair template of your choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs...editing in other organisms and systems, allowing rapid progress in a wide range of fields.   Thank you...genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc...

  16. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from...from the Jorgensen lab. SapTrap is a reagent toolkit and plasmid assembly line for the creation of CRISPR...choosing (Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can be created in a single...single tube. You can also find additional SapTrap plasmids used for introducing point mutations that was published... Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene...PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point mutations with unc...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our...

  17. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...of combining targeted molecular therapies for cancer with chemotherapies, or developing innovative mobile...clinical research to discover and test the best new therapeutics. Consultants often work with such teams to help...human resources towards the development of a new therapy based on its market need and anticipated commercial...context of significant clinical uncertainties and a rapidly evolving healthcare space. The consultants who ...at the core of the suite of benefits a given therapeutic approach brings to patients, doctors, and insurance...and the commercial applications of a particular therapy. With your scientific knowledge behind you, you...business and scientific understanding of successful therapeutics can be a great fit for former healthcare consultants...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... observations: Rapamycin is a membrane-permeable pharmacological agent that can rapidly induction heterodimerization...outer membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP...heterodimerization of proteins that contain rapamycin-binding domains, such as FKBP and FRB. The cell does... the mitochondria (binding FRB), allowing for a rapid and inducible inactivation. Find the Robinson plasmids...approach to control for these effects, drawing on the rapidly growing field of optogenetics. Using a rational... J 2014 Aug 1;33(15):1713-26. RUSH & CRUSH – rapid & conditional gene silencing in RNAi transgenic ...CRUSH alleles off or on enables this method to rapidly address questions of tissue-specificity and cell...

  19. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...successfully helped researchers develop a variety of therapies. For example, many scientists use mice to study...the complex interactions between cancer cells, therapeutic drugs, and the rest of the body than studies ...mutations that made cancer cells more resistant to chemotherapy. You can find these plasmids at Addgene. Another...important human disease model with potential for therapeutic drug discovery as it is estimated that 75% of...they can also be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish (Danio...elegans are small, transparent worms that have a rapid life cycle and large brood sizes. The majority of...Drosophila melanogaster and the role of the fly in therapeutic drug discovery." Pharmacological reviews 63.2...

  20. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...Power., 2019). Their rapid clearance from blood can be a drawback for therapeutic applications where longer...antibodies (Ahmed et al., 2012, Bates and Power., 2019): rapid blood clearance, which is useful for imaging applications...better tissue penetration which is useful for therapeutic and imaging applications; and reduced immunogenicity... longer retention times often increase therapeutic efficacy (Ahmed et al., 2012). How do you generate ...Just like antibodies, there are many scFv-based therapies in clinical trials or already in the clinic (Bates... for cancer, autoimmune, and/or inflammatory therapeutic applications. Antibody fragments such as scFvs...Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 34:404–417 . https://doi.org/10.1089/mab.2015.0036...
Showing: 21 - 40 of 419 results