...operating.  Managing scientists I should add a few special words about managing scientists because, let’s face...the first in a 5 part series on Management for scientists.  “I'm slowly becoming a convert to the principle...special. Below are some special characteristics of scientists in the work place as described by Sebastiano ...I think this is true of most people, not just scientists) Are self-directed, but need precise leadership...flexibility, sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace...series on Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the ... management for scientists...

... AAV9 Biosensor St-Pierre New viral vector pAAV-hSyn-JEDI-2P-WPRE AAV9 Biosensor St-Pierre New viral... constitutively express JEDI-2P, deposited by the St-Pierre lab. Also of interest is the Tian lab's pAAV_hSynapsin_psychLight2...New serotype pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik New viral vector pAAV-nEF-Con...

...bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral...contributed by guest bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre, founders of STEAMid. Internships.... Roo is the co-founder of STEAMid.       Rose C. St Pierre is currently a graduate student at Northeastern...paint a comprehensive picture of your academic interests, potential, and drive. Read the associated prompt...Thus these noble individuals are inundated with requests. The easier you make it for them, the likelier...researchers Applying for jobs? Check out these blog posts  ...

...Plasmid requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 ...plasmids and a total of 2–2,590 requests. We received plasmid requests from 56 countries, though the data...plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests by date...significant fraction of the workforce. Wet lab scientists are no exception to this effect -- meticulously... chose to examine a dataset of Liu lab plasmid requests from Addgene, as a proxy for global activity in...was provided by Addgene on 5/26/20 and contains requests from 5/22/14 to 5/19/20). Each paper has between...Rough estimation of COVID-19 effect on plasmid requests Before we began any sophisticated analyses, we...

...fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot about Mentoring...Mentoring for over 10 years. Many successful scientists describe having a “posse” of mentors as one key ... Stories and Strategies from Successful Women Scientists” by Ellen Daniell. In “Every Other Thursday,”...,” Daniell describes a group of 7 women scientists who met every other Thursday for 25 years. They helped...or to be read or watched during the meeting.  Scientists do better with process, so don’t just get together... style (science). Have a potluck dinner. Invite guests from a lab, career or industry of interest – learn...with any questions about mentoring. Read the other posts in the series to get more advice on mentoring: ...

...the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work... Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs ...potential of Cry proteins in order to manage insect pests and to support sustainable agriculture. For this...then be screened for toxicity against local insect pests. By developing biological agents such as insecticidal...happy to be able to support diverse groups of scientists working in a wide range of research fields and...

...real time (St. Martin et al., 2018). The ACE reporter is a bicistronic construct that consists of a mutated...mediated editing (ACE) reporter in action. Image from St. Martin et al., 2018. APOBEC-Cas9n-UGI targets...been successfully edited with APOBEC3A and APOBEC3B (St. Martin et al., 2018). TLDR: The ACE reporter from...which is not ideal for monitoring single base edits (St. Martin et al., 2018). To circumvent sequencing and... of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019). To create the eGFP reporters...efficiencies using base editing reporters. Image from St. Martin et al., 2019. The Harris lab used these...Reports 9: . https://doi.org/10.1038/s41598-018-36739-9 St. Martin A, Salamango D, Serebrenik A, Shaban N, Brown...

...500,000x Thank You! Addgene's new location: 75 Sidney St, Suite 550A, Cambridge, MA 02139 Have a question?...! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years! We're extremely...extremely proud to be able to support scientists by bringing you all the plasmids you need. We're focused on...world. We've shipped plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries...distribution has grown. Additionally, more and more scientists outside of the US now use the repository – about... so excited to continue to improve access for scientists everywhere to get the tools they need to progress...to ship plasmids, analyze QC sequence, answer scientists' questions, manage our website, and continue ...

...2022.02.13.480251 JEDI-2P voltage sensor The François St-Pierre lab and colleagues have developed a new genetically-encoded...pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE (AAV1) pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2...plasmids is our Fluorescent Biosensors Collection - scientists are always coming up with new tools to measure...

...it? Howarth: Our collaborator Uli Schwarz-Linek at St. Andrews discovered the domain. It is small, highly...

...indicator: JEDI-2P  by: Brian O'Neill  The François St-Pierre lab and collaborators have successfully developed...imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in deep...

...DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT...technique. Lastly, the activity of both antagonists and inverse-agonists could be measured using the TRUPATH...downstream complexes involved, it can be difficult for scientists to understand how GPCR activation leads to downstream...(Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and...and understudied GPCRs in response to different agonists. These studies showed the promiscuity of GPCRs... transducers. The TRUPATH collection provides scientists with a toolkit to gain an understanding of the...blog Browse all of our fluorescent protein blog posts Find other fluorescent biosensors Resources on ...

...Biological Studies The Scripps Research Institute St. Jude Children's Research Hospital  Stony Brook University...turnaround times: less than two days for material requests and less than one week for material deposits. ...they may seem like boilerplate drudgery to some scientists, MTAs are important—they guarantee certain rights...Stopwatch! Any organization that deposits with and/or requests materials from Addgene can qualify. We also reward...agreement just once, covering nearly all future requests/deposits. This reduces the processing time of ...Addgene, whose terms apply to all future materials requests from Addgene MTA: material transfer agreement ...

...CellTag from the Morris Lab at Washington University in St. Louis provides a combinatorial indexing strategy...as single cell RNA-seq (scRNA-seq), now allows scientists to identify the gene expression patterns unique... the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors (iEPs...

...Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype Name 75033...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ...

...have decided to run as The Green Lantern’s friend, St Walker: The Blue Lantern. While The Green Lanterns...

...Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also expanded...easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists can use for...control AAVs to our inventory since we know that scientists need these tools both for pilot experiments and...results of studies. When planning AAV experiments, scientists often have to choose the best serotype for their...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ...

...10.1016/j.cell.2020.12.015 Garcia-Beltran WF, Lam EC, St. Denis K, Nitido AD, Garcia ZH, Hauser BM, Feldman...research. “I hope that by sharing the plasmids other scientists would be able to expand upon the work and continue... Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources on Addgene.org...

... (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema denticola (TD) 3' NAAAAC...

...Court of Appeals. She served as in-house counsel at St. Elizabeth’s Hospital and was associated with Hale...scientific community manage those plasmids so scientists aren’t bogged down with distributing them and...