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We narrowed to 749 results for: SOM

Showing: 381 - 400 of 749 results

  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...has been a powerful tool for quantifying AAV for some time now. When titrating AAV by qPCR, viral DNA ... template control (NTC) well is common. Here are some steps you can take to achieve a clean NTC: Have...is also being used in the detection of microbes. Some applications include measuring viable probiotics...

  2. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...or Adenovirus-Associated Virus type 2 (AAV2) – or some unprecedented combination of the three (Servellita...types (Tatsis et al., 2007), potentially because some of the wild-type viruses can persist as a latent...activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using...

  3. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...though exogenous DNA can be used as well. Below are some considerations for designing donor DNA for successful...shorter homology tracts have recently been used with some success (Yu et al., Nat Chem Biol). Mutate the ...HDR-promoted cycle longer. This approach may have some negative outcomes on viability if cells are arrested...

  4. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...cell biologists talk to chemists? One reason is something called “the photon budget”: each sample has a ...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing...where washing is difficult, such as intact tissue. Some chemical labeling strategies such as the bisarsenical...

  5. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...to analyze product size. This blog post discusses some of the key things to consider when performing colony...amplify a product, then you know there could be something wrong with the PCR setup and/or the primer design...

  6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...versatile protein to suit their experimental needs, and some of the common applications in the lab. Why Green...instead related to the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...as the gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in ...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...questions from scientists all over the world looking for some additional information or clarification on these.... Frequently asked questions Now let's get into some of the nitty gritty and answer the questions those...designed specifically for transient expression (find some useful backbones here) or from viral transduction...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...created a yeast-based directed evolution approach with some notable improvements. Directed evolution techniques...localization of biomolecules within cells. However, sometimes these fluorescent proteins might not localize ...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: New ...

  9. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...ricin uptake. The ricin screen did fail to identify some known ricin-regulators, but most of these genes ...show that genome-wide CRISPR screens are prone to some of the same flaws as RNAi screens, such as non-specific...guides for off-target activity, Morgens et al drew some generalizable conclusions about off-target activity...

  10. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...use epitope tags to monitor single molecules - something researchers often want to do at neuronal synapses...and other FPs have the tendency to brightly label somata but not neuronal processes. In contrast, smFPs ...microscopy. Sample preparation for these techniques sometimes makes it more difficult to detect protein tags...

  11. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...studying vertebrate embryogenesis. To illustrate some of the reasons that zebrafish have been embraced... generating mutants. Unexpectedly, phenotypes sometimes differ depending on whether a gene is deactivated... using lineage tracing in zebrafish To highlight some of the features that make zebrafish useful, I’ll...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...transformation process could save the plant biologist some time… months, in fact. In the Lee-Parsons lab, ...Mortensen et al., 2019). Our method might provide some useful guidelines for the development of transient... different treatments on transformation success. Some reporter gene examples are below. The GUS gene ...

  13. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... the search to a ~4.5 Mbp region of the mouse chromosome. Of the genes located in this stretch of DNA,...permissiveness While PHP.B has impressive CNS transduction in some strains of mice, these results are not reproduced...time AAV user tips from an AAV rookie  Learn about some considerations when using AAVs Learn more about ...

  14. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...evolution of ligands by exponential enrichment (SELEX), some 1010 oligonucleotides could be screened for their...microarrays and biochips. Ultimately, there are just some applications that lend themselves to aptamer-based...Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. ...

  15. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...include the proper protein expression elements (ribosome recognition sequences, start codon, stop codons...attL2 only with attR2, and so on. Take a look at some of the Gateway Multisite plasmids available at Addgene...

  16. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...surface and a corresponding receptor in the host cell. Some of these envelope-receptor pairings are broad, allowing...by nonspecific complement mechanisms in serum. Sometimes this is due to the cells that the viral vectors... broad or specific to suit your needs. Check out some of these review articles to find tables and information...

  17. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...surrounding cell culture over the last few decades some pharmacologists have tended to avoid cell culture... stored in liquid nitrogen long term, preferably some of these vials will be stored in a separate backup...changing but yet more is needed to convince people of some of the basic tenets of responsible cell culture ...

  18. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...difficult, and lacking in reproducibility. Read on for some tips for getting the most out of your lentiviral...virus is the second. When harvesting lentivirus, some labs prefer to collect and pool the harvests from...divided into single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ...

  19. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...potential pitfalls of recombinant protein expression and some of the most popular commercial strains designed ...cell thus leading to slowed growth. Additionally, some protein products may be toxic to the host when expressed...within a reasonable time-frame. For these cases, some strains carry an additional measure of control such...

  20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...oldie but a goodie — a phrase used to refer to something that has fallen out of fashion but is still useful...either in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently expressed using...strong promoters with no tissue specificity, so in some cases they can lead to toxicity and off target effects...
Showing: 381 - 400 of 749 results