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We narrowed to 762 results for: SOM

Showing: 481 - 500 of 762 results
  1. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...journals as those that did make deposits to Addgene.  Insomuch as different journals represent different research...using these weighted numbers. Note: this does drop some journals from our dataset, since not all journals...
  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...Our recent paper (Richardson et al 2016) revealed some of the biophysical parameters that can influence...genetic diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair...
  3. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...' NAAAAC  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system...sequence) to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes...
  4. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...
  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...a process called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction...large-T antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral...
  6. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... should be included and what basic information someone new to antibodies might need to successfully start...separate from the author, and a second review from someone less familiar with the subject for clarity. Our...
  7. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing modifications listed above...applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications, specifically ones...
  8. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding ...off-target effects thus scientists then modified some GPCRs further to respond to specific, pharmacologically-inert...
  9. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...Technologies. If the first thing you hear when someone says they’re a software product manager is “project...sequencing (NGS), and high-throughput screening. While some developers had science backgrounds, most did not...
  10. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome, ...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...
  11. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...Bioluminescent reporters require a chemical substrate (and sometimes cofactors) to be provided and are generally dimmer...as their substrate is available. Table 1 compares some general advantages and disadvantages of each approach...
  12. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...adjusted voltage and compensated your colors. Here are some good practice tips on adjusting voltage: During ...would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity. If your samples...
  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ... gating your target population (b). There is some debate in the literature as to when you need isotype...them to gate our populations, but others do not. Some journals require isotype controls, while others ...
  14. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...number of emerging viral applications and give you some recommendations for putting viruses to work. Viral...targeting an exon in your gene of interest, for example. Some customization is required, but many viral constructs...
  15. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody heavy chain... the lower-case Greek letters μ, δ, γ, ε, and α. Some isotypes have different versions or subclasses that...
Showing: 481 - 500 of 762 results