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We narrowed to 749 results for: SOM

Showing: 481 - 500 of 749 results

  1. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome, ...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...

  5. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody heavy chain... the lower-case Greek letters μ, δ, γ, ε, and α. Some isotypes have different versions or subclasses that...

  6. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...adjusted voltage and compensated your colors. Here are some good practice tips on adjusting voltage: During ...would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity. If your samples...

  7. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...number of emerging viral applications and give you some recommendations for putting viruses to work. Viral...targeting an exon in your gene of interest, for example. Some customization is required, but many viral constructs...

  8. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ... gating your target population (b). There is some debate in the literature as to when you need isotype...them to gate our populations, but others do not. Some journals require isotype controls, while others ...

  9. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... should be included and what basic information someone new to antibodies might need to successfully start...separate from the author, and a second review from someone less familiar with the subject for clarity. Our...

  10. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding ...off-target effects thus scientists then modified some GPCRs further to respond to specific, pharmacologically-inert...

  12. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...a lot of space and don’t tell you why you did something or what the result was, so try not to do this.... that I try to follow in my own research. Again, some of this may not apply or may not work for you. The...

  14. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...like a lot of specialized science, these tools sometimes have limited distribution. We hope that will change...navigate when mapping out a new project. We’ve shared some of the concerns that Revolution Bio had to address...

  16. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... a homologous template provided by the sister chromosome. As cells take those two ends and put them back...there is a possibility of introducing a mutation somewhere else in the genome. It remains to be seen which...

  18. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...order aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking...uploading it as a preprint, or documenting it in some other way. Besides the data, new plasmids and/or...

  19. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...light. Any fluorescent molecule will photobleach at some point. For live-cell imaging, it is desirable to...different ways. We describe a number of issues with some of the experimental designs used to make these measurements...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...strains ultimately perform the same function, there is some evidence that ccdB Survival™ can be more difficult...plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3)...
Showing: 481 - 500 of 749 results