We narrowed to 771 results for: SOM

...contributions.   Our volunteers are extremely diverse. Some of them are undergraduates or PhD students who are...researcher makes a small but crucial contribution to someone else’s project. We think that these collaborations...

...BY fromGroell et.al, 2018.   3D models are somewhat of a middle ground between 2D assays and in vivo...of the adverse drug reaction. This system would somewhat act like a 2-step verification. All approaches...

... look to nature: some protists are coated in symbiotic bacteria (called epixenosomes) that contain an ...

...Our recent paper (Richardson et al 2016) revealed some of the biophysical parameters that can influence...genetic diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair...

...your DNA is intact or the correct size. This is something that absorbance based methods can’t tell you and...ELISA reader if no other instruments are available. Some last reminders about quantifying DNA In choosing...

...technology before the conference begins.“There were some concerns of having a stable internet connection,...We made sure that we had a backup plan in case someone’s internet cut out. “ On networking Networking can...

...journals as those that did make deposits to Addgene.  Insomuch as different journals represent different research...using these weighted numbers. Note: this does drop some journals from our dataset, since not all journals...

...inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months...manipulating specific neurons. We recently packaged a soma-targeted opsin from Christopher Harvey’s lab. According...

...often get positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than... research use only to industry-based scientists. Some organizations choose to share their plasmids under...

...' NAAAAC  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system...sequence) to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes...

...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...

...a process called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction...large-T antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral...

... should be included and what basic information someone new to antibodies might need to successfully start...separate from the author, and a second review from someone less familiar with the subject for clarity. Our...

...cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing modifications listed above...applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications, specifically ones...

...constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody heavy chain... the lower-case Greek letters μ, δ, γ, ε, and α. Some isotypes have different versions or subclasses that...

...number of emerging viral applications and give you some recommendations for putting viruses to work. Viral...targeting an exon in your gene of interest, for example. Some customization is required, but many viral constructs...

...a lot of space and don’t tell you why you did something or what the result was, so try not to do this.... that I try to follow in my own research. Again, some of this may not apply or may not work for you. The...

...Bioluminescent reporters require a chemical substrate (and sometimes cofactors) to be provided and are generally dimmer...as their substrate is available. Table 1 compares some general advantages and disadvantages of each approach...

...Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome, ...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...

...RNA sequence (in this case, norovirus RNA), the ribosome binding site is inaccessible. When the target ...however, it binds to the switch and exposes its ribosome binding site, allowing for the gene on the toehold...