We narrowed to 791 results for: REP

...responsible for guiding new deposits into the repository, performing quality control, and providing technical...listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom. I realized I could and should be...like kudzu. Think of a soccer player who gets a reputation for taking risky shots at the goal rather than...

...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...depends on a variety of factors including sample preparation, staining procedures, and controls. To prevent...multicolor flow cytometry experiment, users must also prepare sets of controls to aid with compensation. Compensation...

...Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned 728 articles (3/12/2014). With...Gene Conversion Tracts from Double-Strand Break Repair in Mammalian Cells. Molecular and Cellular Biology.... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...

...reagents including plasmids, ready-to-use viral preps, pooled libraries, kits, and cloning grade DNA! ...100,000 different plasmids available from the repository (currently at 98,276 plasmids as of April 8, ...

...Can’t find a plasmid you’re looking for in the repository? Let us know! You can notify us to reach out ...depositing, we hope this campaign will expand the repository in ways that will be most useful to you, the ...

...Now we have over 8,000 CRISPR plasmids in the repository that have been shared over 140,000 times to over...applications (such as visualize, screen, activate, and repress) on Thursday. We’d also like to see what you think...

...fields.  Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...

...el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas...help increase global access to materials in our repository. This incredible gift will allow more people ...

...introduced us to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh...excellent Q&A from Tim Miles on everything from AAV prep to targeting to animal models. It was one of our...

... a trick to get the highest yield out of your minipreps? If so, we’d love to have you write for us! Click...molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development...

...our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent distribution...quest. Keep an eye out for Abi as they help us prepare for our upcoming antibody launch in late March!...

...: How Crystal advanced at Novartis, and how to prepare yourself for success by having a development conversation...learning from peers, and being receptive to those who report to you. Chapter 5: The types of science that Crystal...

...longevity. 10:56 - 12:00 : How Alex's work in DNA repair informed his decision to work with CRISPR during...Koeris Explains His Path from Grad Student to Entrepeneur Find Resources to Help You Choose Your Career...

... the scientists who deposited plasmids to the repository and for the scientific sharing community (check...started a new service distributing ready-to-use viral preps in 2016 and we just reached our 10,000th viral vector...

...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...

...through the lens of Addgene’s blog and plasmid repository. Our blog is just over a year old, and it has...Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making...

...resources: Preserving scientific assets: We hold a repository of valuable research materials that might otherwise...offer high-quality DNA sequencing, viral vector preparations, and antibody production that labs may find ...

... SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or...control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor...

...wide variety of science careers that Addgenies represent. They kicked off their day with breakfast and ...see a real working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite...

... veritable virus factory.  Their findings were reported in the Journal of Virology.  The EBOV glycoprotein...offers sequence data on five strains of Ebola representing the latest outbreak and earlier ones, along ...