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We narrowed to 791 results for: REP

Showing: 601 - 620 of 791 results
  1. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...of Research on Women’s Health, emphasizes that reporting the sex of cells and tissues—when that information...diagnostics and therapeutic interventions you develop represent the whole human population. To learn a little ...
  2. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...development to mentoring for women and under-represented minorities. Her online content is of great value...
  3. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo...
  4. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...problems with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan... to mouse modeling now complete, you should be prepared to plan an experimental strategy that uses genetically...
  5. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...lot to lot specificity and tend towards more reproducible results. Since polyclonal antibodies contain... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...
  6. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...finally, to expert.   Figure 1. Visual representation of common injection routes in mice. Image created...events. Intradermal injections require a bit of extra prep, as the injection site area must be shaved first...
  7. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...transcripts if requested Thank them, and eagerly await a reply. It’s a good idea to email around three professors...about your research goals and education, too! Prepping for the interview Many universities offer free...
  8. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology...
  9. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...work, and it begins with the central dogma - replication of DNA, transcription of DNA to make RNA, translation... tame the rest of a cell. The BIOFAB plasmids represent the first systematic collection of genetic parts...
  10. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ... programmability of CRISPR-Cas, these systems represent the next generation of tools for efficient and...genomes engineering In the Sternberg group’s initial report of VchINT, three separate plasmids and multiple...
  11. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining {%...noiseless) model for our data: We choose to let yx(t) represent the number of unique PIs or labs placing orders...
  12. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...cell types, gene editing using homology directed repair (HDR), and generating large genomic deletions. ...delivery. You’ve decided to use RNPs, now what? Preparing the Cas9-gRNA ribonucleoprotein The first step...
  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...club? Where will the journal club take place? Preparing for the first meeting There are many important...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...
  14. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...space where you can address the issue head on. Prepare for this time as if it were a meeting. Gather the...change with them. If you can, set aside time to prepare for future challenges. This doesn’t have to be ...
  15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...and triggering release of the stress hormone norepinephrine to promote vigilance. A recent study investigated...those neurons are chemogenetically activated, norepinephrine release causes a freezing response, a form ...
  16. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...package. There are three steps to chalk talk preparation: Define your research vision. Organize your ...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach...
  17. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... abundance relative to cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...
Showing: 601 - 620 of 791 results