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We narrowed to 777 results for: REP

Showing: 601 - 620 of 777 results

  1. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ... programmability of CRISPR-Cas, these systems represent the next generation of tools for efficient and...genomes engineering In the Sternberg group’s initial report of VchINT, three separate plasmids and multiple...

  2. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...space where you can address the issue head on. Prepare for this time as if it were a meeting. Gather the...change with them. If you can, set aside time to prepare for future challenges. This doesn’t have to be ...

  3. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...and triggering release of the stress hormone norepinephrine to promote vigilance. A recent study investigated...those neurons are chemogenetically activated, norepinephrine release causes a freezing response, a form ...

  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...package. There are three steps to chalk talk preparation: Define your research vision. Organize your ...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...

  7. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...targets can be done largely in vitro; Easy and reproducible commercial synthesis (those of us who have used...highly customizable in terms of how they actually “report” as biosensors. Making aptamers glow The flexibility...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ... overall structure of 13 base pair palindromic repeats separated by an 8 bp asymmetric core. Recombination...Katherine L. et al. 2016. “Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In ...

  9. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...becomes available. For routine infections, like strep throat, testing for antibiotic resistance isn’t ...Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & ...

  10. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... abundance relative to cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...

  11. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647–661 . https://doi.org...on Addgene.org Find Cas9 Activators Find Cas9 Repressors Catch Up on Your CRISPR Background with Our CRISPR...

  12. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...able to ask follow up questions, or ask people to rephrase questions was key. I had also hoped that just ... 5: The mobile monitor and teleprompter being repurposed for a different shoot to create the How to Deposit...

  13. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... giving birth - of about 10 weeks. Adult mice reproduce as often as every three weeks so scientists can...important gene co‑expression modules." Biomedical Reports 7.2 (2017): 153-158. PubMed PMID: 28804628. PubMed...

  14. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...interest with an additional protein module that can report on location. This approach enables the study of...9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ...

  15. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...risks associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the...community is the ultimate value proposition. By preparing a budget before hand, you’re saving the institute...

  17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... This will help to ensure the accuracy and reproducibility of the data and can instill greater confidence...Identity of Recombinant Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy ...

  19. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ...

  20. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...not result in a reward sensation and subsequent repeated stimulation. A spatial memory task, on the other... your injections until they are accurate and reproducible. I also highly recommend spending the money ...
Showing: 601 - 620 of 777 results