We narrowed to 791 results for: REP

...lanes 4-5), there is a single band of 7.3 kb representing the full size of the plasmid. The double digest...

...the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

..., 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction efficiency can have a major ...

...interest. Using isotype controls An isotype sample is prepared very similarly to a FMO sample: you make a sample...

...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...

...becomes limited, which can be a threat to future reproducibility. The Trimmer Lab found that in addition to ...

...not just the antibodies that matter when you’re prepping for your experiment - there are a number of outside...

...valency of 10 antigens. An extra constant domain replaces the hinge region in IgM antibodies. IgM has no...

... efficiently. For select viral vectors in the repository, you can instead have Addgene make the virus ...

...important resource that Addgene provides is an open repository of plasmids and other necessary information that...

...plasmid from the Zhang lab expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...

... The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged...

... for each analysis or task will keep you from repeating your work or making similar mistakes in the future...

...factors. The second vector is a homology directed repair donor plasmid with homology arms and a selection...

...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

...comprehensive plant cloning kits available in the repository, MoClo and GreenGate, and we are excited to see...

... the question, you need only to take that step. Repeat that as often as needed until you get to the end...

...different samples and experimental conditions. Be prepared to troubleshoot and be sure to include proper ...