We narrowed to 777 results for: REP

1. How to Keep a Lab Notebook for Bioinformatic Analyses

   Type

   Blog Post

   Published

   June 2, 2016, 2:30 p.m.

   ... for each analysis or task will keep you from repeating your work or making similar mistakes in the future...

2. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...factors. The second vector is a homology directed repair donor plasmid with homology arms and a selection...

3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

4. 3 Challenges in Plant Synthetic Biology

   Type

   Blog Post

   Published

   July 22, 2014, 5:46 p.m.

   ...comprehensive plant cloning kits available in the repository, MoClo and GreenGate, and we are excited to see...

5. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ... 101: Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in human cells...

6. Celebrating 15 Years of Scientific Sharing

   Type

   Blog Post

   Published

   Jan. 14, 2019, 2:08 p.m.

   ...candidates from internal referrals. Our long reaching reputation helps us hire great people from farther away ...

7. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   Published

   March 15, 2022, 1:15 p.m.

   ...different samples and experimental conditions. Be prepared to troubleshoot and be sure to include proper ...

8. Transferable Skills Guide: Public Speaking

   Type

   Blog Post

   Published

   April 20, 2023, 4 p.m.

   ... the question, you need only to take that step. Repeat that as often as needed until you get to the end...

9. Antibodies 101: Conjugation

   Type

   Blog Post

   Published

   March 26, 2024, 1:15 p.m.

   ... but it has a strong affinity for avidin and streptavidin, both of which can bind up to four biotin molecules...

10. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...miscommunication and b) find practical solutions.  My PhD prepared me remarkably well for this task. Good scientists...

11. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...Other steps fail “soft” they simply need to be repeated. We also now have a good interface to begin tracking...

12. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...Homolog Cpf1 Read How Non-Homologous End Joining Repairs Double Strand Breaks Get Tips on Designing Your...

13. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...running these events will be important both in preparing you for future work in these areas, and in helping...

14. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...riboprobes are created, the WISH protocol involves preparation of the tissue sample, several washes, and temperature...

15. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...and labeled protein interactors purified via streptavidin pulldown. Following trypsin digest, PPIs are...

16. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...to be creative. After all, your poster is a representation of your work and a reflection of you, so make...

17. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...list. 7) Internships – Learn on the job Nothing prepares you better than learning on the job. Try to get...

18. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell ...

19. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you...

20. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...SpCas9, is NGG. Assuming all nucleotides are represented equally, that’s only a 1/16 chance of having ...