Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 419 results for: RAP

Showing: 61 - 80 of 419 results

  1. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...of text. Since our guides are heavily used, our graphic designer made most of the Antibody Guide figures...communication between our content team and our graphic designer to make sure things are clear, accurate...Fig. 2: Reference image (left) provided to our graphic designer upon request that the science communicator...design (right). Immediate and immense regret of the graphic designer not shown.   However, for our blog...retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. Thus, we are constantly...

  2. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...outlined below as well. Cas12a also has significant therapeutic promise due to the fact that it is smaller than...simplicity of Cas12a makes it easier to package for therapeutic delivery by AAV, which has strict capacity limitations...sequences of Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement...TTTN. Best for: homology-directed repair or therapeutic delivery.   Cas14 – Mini, but single strand ...stirred up interest for its potential as a viral therapeutic that could specifically degrade target viral ...

  3. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...analysis of the US Women’s Health Initiative Hormone Therapy Trial, for example, found that the study saved ...Reduced use of estrogen plus progestin hormone therapy resulted in 76,000 fewer cases of cardiovascular... cells and tissues, specifically in stem cell therapies. Let’s go back to why ten drugs were withdrawn... shows that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers... both sexes ensures that the diagnostics and therapeutic interventions you develop represent the whole...

  4. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...your study is aimed at developing human therapies. If a therapeutic is the end goal, it is best to work with...Targeting is also important in the development of therapies when expression must be restricted to specific...Varenicline has been further modified to create ultrapotent PSEMS (uPSEMs) that have drastically increased... A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research and potential clinical...

  5. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ... that CRISPR technology is consistently showing rapid development and quick adoption by the community....request and use new tools. Because there’s such rapid adoption, we thought that requests would be biased...CRISPR tools that fill needs in the community. The rapid adoption of new tools, illustrated in Figure 2, ...usefulness of popular CRISPR tools is both enduring and rapidly recognized.   Where is the CRISPR community?  Addgene...looked at depositors and requestors by country, and graphed the top ten countries for each group (Figure 4)...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...vectors: The Rubik’s cube of human gene therapy. Molecular Therapy, 30(12), 3515–3541. https://doi.org/10.1016... vector for gene therapy of human diseases. Signal Transduction and Targeted Therapy, 9(1). https://doi.org... liver, interrogate immune cells to improve immunotherapy in glioblastoma, and generate programmable screening...

  7. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...mutations do arise and cancer ensues, traditional chemotherapies have taken advantage of the universally-dividing...Further, depending on the specific type of cancer, chemotherapy is not always effective. This is why recent ... They have proven very difficult to target therapeutically due to their unusually “smooth” shape and lack... high-affinity binding targets for potential therapeutic agents. Nevertheless, a glimmer of light remains...pathways. The curation project continues to evolve rapidly and, with assistance from the research community...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... Adenovirus Vectors for Gene Therapy, Vaccination and Cancer Gene Therapy. Curr Gene Ther, 13(6), 421–...was first isolated from human adenoid tissue and rapidly caused the death of adenoid cells in vitro (Rowe...oncolytic virus in addition to standard uses in gene therapy and in basic research, like other viral vectors...novo viral gene expression: Implications for gene therapy. Proc Natl Acad Sci U S A, 95(19), 11377. https...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...valuable tool for microglia research and broader therapeutic applications.  Find microglia-targeting AAV plasmids...10.1101/2023.12.12.571321.  Revolutionizing Gene Therapy: The REVeRT Dual AAV Vector System By Vaibhav Kawde... typically associated with viral vector-based therapies. REVeRT supports advanced gene expression regulation...mechanisms of delivery and the potential for therapeutic success. Find REVeRT plasmids here! Riedmayr...dual AAV vectors for (epi)genome editing and gene therapy. Nat Commun., 14(1), 6578. https://doi.org/10.1038...

  10. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...Reference CBE SpCas9 rAPOBEC1 NGG 6 SpCas9 Target-AID (PmCDA1) NGG 5 SpCas9-VQR rAPOBEC1 NGA 8 SaCas9-... plant systems. Target-AID based (CBE-2)-  and rAPOBEC based (CBE-1)-  CBEs are successful base editors...rice, tomato, wheat, and potato. Target-AID and rAPOBEC based CBEs are known to deaminate cytosine placed...SaCas9-KKH rAPOBEC1 NNNRRT 8 ABE SpCas9 TadA-TadA* NGG 9-12 SpCas9-VQR TadA-TadA* NGA 8 SaCas9 TadA-TadA...

  11. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...opened up vast possibilities for the clinical and therapeutic applications of iPSCs. The generation of human...obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find stem cell ... The phenomenon has been recorded in past gene therapy trials and remains a safety concern in the clinical... cell proliferation in two patients after gene therapy for SCID-X1. Science, 2003. 302(5644): p. 415-9...Ehrhardt, A., et al., Episomal vectors for gene therapy. Curr Gene Ther, 2008. 8(3): p. 147-61. PubMed ...

  12. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...genome-containing (Figure 1).    Figure 1: Electron micrograph of an Addgene AAV viral vector preparation after... and improved alternative methods." Human Gene Therapy, Part B: Methods 23.1 (2011): 1-7. PubMed PMID:...can limit production of recombinant AAV-2." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, ...type 2 Reference Standard Material." Human gene therapy 21.10 (2010): 1273-1285. PubMed PMID: 20486768.... infectivity of recombinant AAV vectors." Gene therapy 17.7 (2010): 872-879. PubMed PMID: 20336156. PubMed...

  13. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol bath or liquid...processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology Advances 24.3 (2006). 321...lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development 25.3 (2014). 175-85. Pubmed...lentiviral vectors through pseudotyping.” Current Gene Therapy 5.4 (2005). 387-98. Pubmed PMID: 16101513. PubMed...predicting gene transfer events.” Genetic Vaccines and Therapy 2.1 (2004) Pubmed PMID: 15291957. PubMed Central...

  14. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...inserted downstream of a functional promoter (gene traps). The resultant gene targeting system could be used...the efficiency of homologous integration via gene trap enrichment, whereas pAAV-TK-Acceptor has a conventional... in human pluripotent stem cells." Molecular Therapy20.2 (2012): 329-338. PubMed PMID: 22108859. PubMed...DSB-mediated gene editing technologies." Current gene therapy 16.3 (2016): 207-219. PubMed PMID: 27280971. 10...nucleases with adeno-associated viral vectors for therapeutic gene editing." Precision Medicine, CRISPR, and...

  15. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...and W describe the shape of a histogram, which graphically represents the time and intensity of the cell...the detected photons can be represented through a graph of photocurrent vs. time. The resulting histograms...expected size. The strong signals close to the graph’s origin mainly stem from cell debris and other small...wondering which second measurement to choose for graphically displaying your events. You are free to choose...T killer cells but not dendritic cells. Using a graph displaying both stains, I can quickly separate T...

  16. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...coming of age. Molecular Therapy : the Journal of the American Society of Gene Therapy, 17(8), 1316–1332. https...clinical settings, as evidenced by retroviral gene therapy trials for X-linked severe combined immunodeficiency...lentiviral vectors: progress and applications. Gene Therapy, 17(2), 150–157. https://doi.org/10.1038/gt.2009.135...mutations causes leukemogenesis following gene therapy of SCID-X1 patients. The Journal of Clinical Investigation...

  17. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...opening and allowing positively charged ions to rapidly pass through the cell membrane and initiate an ... their experiments. Some tools turn on and off rapidly with light to control precise timing of the perturbation...activation kinetics Some channels turn on and off very rapidly in response to a single light pulse whereas others...responses (Gunaydin et al., 2010). This can affect how rapidly you can activate or inhibit a cell in succession...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they...

  18. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ...not right for the development of phage-based therapeutics. After a brief stint at the venture capital ...Novophage's focus. The company went from developing therapeutics to developing phage-based tools for detecting...develop tests for food products as opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens...for Dr. Koeris to transition from developing therapeutics to developing a diagnostic? 36:53 - 38:00 : ...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...fragment with FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting in a rapamycin-inducible Cas9...Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible...Cas9 for genome editing. Without rapamycin treatment, the Cas9(N)-FRB-NES fragment is actively shuttled...to the nuclear export sequence. Treatment with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS...splicing   The Gideon Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter...e.g. fluorescence recovery after photobleaching [FRAP]) for studying membrane dynamics, for monitoring...

  20. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ... Figure 2: A bacteriograph of Michelangelo's "The Creation of Adam." This bacteriograph was created using...Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical abstract, it’s a musical abstract,” says Di Ventura...). These images are based on the concept of bacteriographs created about a decade ago. To create these...
Showing: 61 - 80 of 419 results