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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 419 results for: RAP

Showing: 81 - 100 of 419 results

  1. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...advancement of SARS-CoV-2 research because they provide rapid access to validated reagents, especially during ...nonprofit research institution, has been able to rapidly propagate this strain, making it available to scientists...provides. Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing logistic...hurdles to continue making materials available rapidly to scientists (Nature Biotechnology 2020). The ...

  2. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...your writing habit into beautiful graphs! I like to look at these graphs when I need that extra push to ...to stick with my writing routine. The graph of which days of the week you most often completed your habit...flagging passive voice, overuse of adverbs, and paragraphs with many hard-to-read sentences.  Grammarly ...

  3. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...mRNA. These RNA tools have been used in clinical therapy to target pathogenic proteins, as well as in the... In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing radiation, can also result...responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount of siRNA...just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease, and much more...

  4. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism, but their small packaging capacity...tested the base editors in vivo and achieved therapeutically relevant editing efficiencies in the mouse ...comparable to those currently used in human gene therapy trials. They further tested the base editors by...Niemann-Pick disease type C, directly demonstrating the therapeutic potential for the split base editors. Find the...

  5. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest, increasing target...system as we can use it to evaluate the effects of rapid and selective degradation of protein targets in ...machinery through binding with cereblon, leading to rapid target degradation (Figure 1). Plasmids: We generated...tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal...

  6. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...levels? Until recently, no such tool existed for rapid, customizable protein degradation. Enter TRIM-Away...antibody targeting the protein of interest led to rapid, targeted degradation that is dependent on proteasome...targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et...FIRE–Cas9), combines these approaches to enable rapid and reversible recruitment of chromatin complexes...

  7. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... only for translational research, such as gene therapy, but also basic research. To improve cell-type ... with high rates of labeling are ideal of gene therapy applications, promoters that produce sparse labeling...basic as well as translational research for gene therapy. The Roska lab hopes to design improved versions...blog Read more about using AAV for retinal gene therapy Find more about precise neuronal targeting using...

  8. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...perfectly reasonable paragraph unintelligible to a lay audience. My lay paragraph typically includes a...the best place in the world for your research to rapidly progress. Need Help Building Your Scientific Network...kindly friends and family to read my non-expert paragraph and point out words or sentences that are unclear...

  9. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...article ultimately reported the development of a rapid design and prototyping pipeline that can take pathogen...the Zika virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus research...other critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future...sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection of Zika Virus Using Programmable...

  10. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...models for developmental processes and promising therapeutic tools. The two unique characteristics that define...cells can be used for different research and therapeutic purposes. Both types have their advantages and...of post-transplantation rejection when used therapeutically High Low (if ASCs are isolated from the patient...directly from a patient’s adult cells when used therapeutically, the chances of post-transplantation rejection...

  11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...sensitization and might open new ways for a novel therapeutic approach against drug addiction. Anxiety tests...pro-inflammatory cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN), a region thought to be involved in... medial prefrontal cortex Drd1 neurons produces rapid and long-lasting antidepressant effects." Nature...., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation promotes dramatic behavioral stress...

  12. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...One of the biggest barriers to gene therapy treatment of central nervous system (CNS) diseases is the...Transport across the Blood-Brain Barrier." Molecular Therapy 27.5 (2019): 912-921. PubMed PMID: 30819613. Hordeaux...AAV-PHP. B are limited to C57BL/6J mice." Molecular Therapy 26.3 (2018): 664-668. PubMed PMID: 29428298 PubMed...dose–response and comparison to AAV PHP. B." Gene therapy 25.5 (2018): 392. PubMed PMID: 30013186. Matsuzaki...

  13. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...toxins to facilitate efficient recombineering (Khetrapal et al., 2015). They created vectors for Lambda...sacB cassette with one of three toxins from the Khetrapal paper. We selected the three toxins so that at...control of a rhamnose-inducible promoter, though Khetrapal et al. also demonstrated that the arabinose promoter... 23360965. PubMed Central PMCID: PMC3748948.  Khetrapal, Varnica, et al. "A set of powerful negative selection...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...they circulate in the blood, rather than being geographically constrained to the brain. In cases where it...underexplored sites on the AAV capsid. Molecular Therapy - Methods & Clinical Development, 26, 331–342. ...for Robust CNS Transgene Expression. Molecular Therapy - Methods & Clinical Development, 15, 320–332. ...-PHP.B Are Limited to C57BL/6J Mice. Molecular Therapy, 26(3), 664–668. https://doi.org/10.1016/j.ymthe...Transport across the Blood-Brain Barrier. Molecular Therapy, 27(5), 912–921. https://doi.org/10.1016/j.ymthe...Non-human Primates Compared with Mice. Molecular Therapy, 27(11), 2018–2037. https://doi.org/10.1016/j.ymthe...

  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...are released in each synaptic event and they are rapidly taken up by postsynaptic receptors and transporters...sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex...much to learn about their myriad functions and therapeutic potential. Our partners at the Institute for ...critical — factor for successful experiments or therapies. Using AAV vectors with enhancers that drive cell-type-specific...

  16. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...of the DA neurons produce behavioral and electroencephalography evidences of arousal in mice previously...enabled the drawing of a detailed and functional topography of the neural circuit architecture of the brain...exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological Disorders. Humana Press, New York...26135556. Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical...

  17. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional....2020.100135. Fonseca, João Pedro, et al. “A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian...Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors...Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies. Cold...

  18. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...software could also be used as a simple tool to rapidly find one or a few gRNAs suggested to be effective...appropriate phenotypic readout, similar mapping could be rapidly performed by targeting all potential components...identified for each gene. This file can be used to rapidly get an overview of the list of gRNAs generated....screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia." Cell reports...

  19. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    .... TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger nucleases to transcription... 10 years of plasmid sharing? Check out this infographic created by our own Addgenie, Laura Veckerelli...Veckerelli. Click on the picture below to see the full graphic.        ...

  20. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke University Medical Center...identify those that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said... cancer therapy...
Showing: 81 - 100 of 419 results