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We narrowed to 570 results for: RON

Showing: 161 - 200 of 570 results

  1. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery...tagging endogenous proteins with an auxin-inducible degron (AID) tag to generate proteins which can be rapidly...Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.” Cell Rep.15... Michelle Cronin...

  2. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...many researchers remain unaware that EGFP/GFP is prone to forming noncovalent dimers. This property of ... matter of concentration Most FPs in nature are prone to dimerization (i.e. EGFP) [1, 2] or even form ... fused to the POI. These structures are large (microns), bright, non-physiological, and difficult to ignore...CFP and YFP dimers can form in the absence of environmental changes that the biosensor is designed to detect...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...change their excitation/emission behavior upon environmental changes (e.g. pH, Ca2+ flux, etc). The most ...Oligomerization: The first generations of FPs were prone to oligomerize. This may affect the biological function... these FPs cannot be used in oxygen deprived environment. Recently, a new GFP isolated from the Unagi ...you are planning to express the FP in acidic environments (e.g. yeast cytosol, which is slightly acidic...

  4. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...either DTT or β-mercaptoethanol (BME). DTT is a stronger reducing agent than BME and has a less… distinctive...life when stored properly. Because BME is not as strong as DTT, you’ll have to use different concentrations...quantifying your blot. CCD cameras have a much higher upfront cost, but require no consumables, produce digital...like Coomassie Blue or Ponceau S. It is far less prone to error than normalizing to so-called housekeeping...happy blotting! Many thanks to Addgenies Ashley Waldron, Meghan Rego, and Amrita Rhodes for their help ...

  5. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...refresher if you ever lose track of which acronym is which. Acronym Full name Notes   Base transition ...close at hand? Once again, this post has a lot of acronyms, so we've included a glossary at the end of this... for saturation mutagenesis, and they provide a strong proof of concept for further development. ...

  6. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...contains iterons that serve as recognition sites for replication initiation proteins. These iterons vary ...plasmid with multiple oris that vary in DnaA-box and iteron structure, host-independent   Building broad...Development of constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia...

  7. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...experimental systems. They demonstrated LOV-Turbo in neurons, in pulse-chase experiments, and more. Find LOV-Turbo...element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator gave fluorescent...Images of iPAK4 fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at... Teaching an old antibody new tricks By Ashley Waldron While IHC protocols typically call for thin sections...

  8. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...Boyden (Addgene viral prep # 84445-AAVrg).   Electron Microscopy The ratio of empty to full (i.e., genome-containing...representative vector preparations was quantified with electron microscopy after negative staining. Empty vector...than full vector particles.     Figure 3: Electron micrograph of AAV vector preparation shows that...psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell culture medium (OptiPro SFM) containing...

  9. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...DNA dot blot...Transduction assay...SDS-PAGE...Electron microscopy… Any guesses? That's right... ways...genome-containing viral capsids is typically quantified by electron microscopy of a viral vector solution. Since this... this technique is intensive and requires an electron microscope, it is not routinely performed on all...genome-containing (Figure 1).    Figure 1: Electron micrograph of an Addgene AAV viral vector preparation...

  10. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...pluripotent stem cells (Chavez et al., 2015) into neuronal cells by activating genes that cause differentiation...CRISPR-based activation can be made inducible upon environmental triggers. This is done by creating a split dCas9...CRISPR activation are also reversible: once the environmental trigger leaves, gene expression is no longer...Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ (2019...

  11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... methods.   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay...in the saliva (Lucia et al., 2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​...ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter...2020). An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on...

  12. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...reports 5 (2015). BIOFAB plasmid set 3000 Series Confronted with the problem of creating vectors for predictable...Shine-Delgarno sequences used in this so-called bicistronic design (BCD). The 49 vectors in this kit can ...of varying strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et al., 2013...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...

  13. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...aggregation in your prep. If your proteins are the wrong size on your gel or blot, Table 1 (at the end of...and keep an eye on it and the leading line (dye front) visible in the sample buffer. You’ll want to run...interest, but you don’t want to run so long your dye front or ladder runs off the gel. Luckily, this is easily...easily monitored in real time, as the dye front (and many ladders!) are visible to the naked eye.   If ... Troubleshooting Suggestion Proteins are the wrong size Proteins may be aggregating Lyse at lower...

  14. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...to reduce false positives within a test.   The Bonferroni Correction and the Benjamini-Hochberg procedure...control for large numbers of comparisons. The Bonferroni does this by establishing a significance threshold...However, if many comparisons need to be performed, Bonferroni and Benjamini will greatly reduce family-wise...Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume 2 (2016). doi.org/10.3389...

  15. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...Heinrichs, 2009). When a fluorophore absorbs light, electrons are elevated from a ground state to an excited...After a brief elevation in this excited state, the electron returns to its ground state by emitting a photon...Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers, R., Bousfield, D., & Demokritou, P...nanocellulose for nanotoxicological studies. Environmental Science: Nano, 6(5), 1516–1526. https://doi....

  16. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ... Studying cell mobility within these complex environments has never been easy and, for many years, researchers...same laser) cooperate to cause a higher-energy electronic transition in a fluorescent molecule (see figure...adaptive and innate immunity in diverse tissue environments. These studies provide quantitative measurements...-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii." The Journal...

  17. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ... (3): An optics system A fluidics system An electronics system  Cells are first resuspended in a pressurized...fluorophore or fluorochrome-stained protein will emit a stronger photon than a cell expressing low levels of the...triton-x, saponin, or tween. Take care when using strong detergents like triton-x and tween as extended ...FACS In addition to the fluidics, optics, and electronics systems, FACS instruments have specialized components...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...in E. coli by co-expressing the molecule chaperone Skp. Front Cell Infect Microbiol 3: . https://doi.org...Antibodies: Historical Perspective and Future Outlook. Front Immunol 8: . https://doi.org/10.3389/fimmu.2017.01589...biosensor biorecognition elements. Biosensors and Bioelectronics 85:32–45 . https://doi.org/10.1016/j.bios.2016.04.091...

  19. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...many scientists operate in a hyper-competitive environment where getting hired hinges on performing near...government scientists to the de-funding of crucial environmental research programs, Canada has been here before...researcher in the University of Michigan Department of Astronomy. She studies how planets form around young stars...community of science supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is ...

  20. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ... present in the sample. Other techniques, like electron microscopy and FRET, can offer more information...well described in the closed conformation, recent electron microscopy studies have also found Cas9:RNA complexes...pairing in the seed sequence.   Figure 6: Electron microscopy structure of S. pyogenes Cas9 bound...structure (left, PDB ID 4ZT0) with Cas9:RNA:DNA electron microscopy structure (right, PDB ID 7S4X), viewed...

  21. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...particularly energy-intensive systems in a more environmentally responsible manner.  Other ways to make the...in-depth look at Addgene’s current lab-oriented environmental efforts check out Leila Haery’s blog series,... we're always striving to set an example of environmental stewardship.  Additional resources on the Addgene...

  22. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ... connections between BLA projection neurons and mPFC interneurons can be labelled with an AAV1 Cre virus...AAV9 tdTomato virus in the mPFC.  The AAV9 is so strong that you can even see dendritic spines!  Curious...

  24. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...Chinmaya Sadangi, a postdoc at the University of Toronto. The Addictive Brain was founded in early 2018 ...Chinmaya Sadangi, a postdoc at the University of Toronto, created The Addictive Brain to spread awareness...Sadangi is currently a postdoc at the University of Toronto and founder of The Addictive Brain. Prior to this...

  25. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...ways that I could change the world around me. My strong interest in the intersections of media, art, and...forward but I have learned to push myself to the forefront, no longer allowing myself to just simply exist... an Addgenie. I thought about all that could go wrong with this internship but in the end it turned out...

  26. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...Moriarty, an Associate Professor at the University of Toronto, co-founded the COVID-19 Resources Canada site....groups have focused on iterating on methods for coronavirus research, sharing resources for COVID-19 research...our eye recently (not a comprehensive list). #coronavirus #SARSCoV2 #Covid19researchA thread ⬇️⬇️⬇️ — Addgene...

  27. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...the workforce, but my main concerns were building strong connections with coworkers and doing something ...to engage with the work of others. Having an environment filled with people who are interested in understanding...interested in knowing you as a person, builds a strong sense of trust between coworkers. Since I trusted...

  28. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f in the...optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an optogenetic tool...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/...

  29. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first thing you'll...you need to know is your expression system or environment. The host organism will determine the type of...overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression vectors are...

  30. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    .... In neutral environments, dimeric Keima is excited at 440 nm and in acidic environments at 586 nm. Regardless...mechanism as Rosella: GFP is quenched in acidic environments, while mRFP is relatively stable and fluorescences...

  31. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits. ...information resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved...

  32. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...and chemogenetics communities to activate neurons and neuronal pathways using light and chemicals respectively...machines that can extend and retract over many microns in response to pH changes without the need for ...

  33. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...already available allowing you to chemically control neuronal activity while studying your phenotype of interest...interest. Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We...we have retrograde AAV that you can use to map neuronal connections. Expanding the viral vector knowledge...

  34. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...including neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and ...and cell death post-transduction is low. Neuronal stem cells transduced with iTOP resulting in GFP...

  35. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...PMC9015817.   Meet anti-GFP [N86/8R]! by: Ashley Waldron Anti-GFP [N86/38.1R] was among the first batch ...microscopy applications such as super resolution, electron, and expansion microscopy. hfYFP is not only brilliantly...expansion microscopy as well as correlative light and electron microscopy and observed high fluorescence resilience...

  36. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar... are urgently needed. Therefore, understanding neuronal networks, their interactions and information processing...microscope will allow Tsakanova to image cortical neurons and study changes in neural activity after ischemic...

  37. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...to graduate from university, many students are confronted with the question ‘what now?’ This is often a...good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel, PowerPoint...exercise these skills outside of a formal work environment also shows that you are passionate and capable...

  38. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...Predict Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389/fimmu...Infrastructure and a Vision for Future Enhancements. Frontiers in drug discovery, 2, 952326. https://doi.org/...

  39. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...abstract comparing two fictional medical issues – chronic and acute “adeolitis” – received the best ratings...greater levels of science literacy are especially prone to this, possibly because a greater familiarity ...cloth menstrual pads while we talked about the environmental impact of disposable products. A great deal ...

  40. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...to create a more productive and cohesive team environment. Neglecting communication skillsClear communication... great "baby project," offering a supportive environment to build my management skills. Finally, developing...effectively and foster a cohesive, productive environment. For example, in my current role managing a cross-functional...
Showing: 161 - 200 of 570 results