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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 419 results for: RAP

Showing: 101 - 120 of 419 results

  1. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...reason this research has been able to progress so rapidly is scientific sharing. Already we’ve seen articles...and data associated with COVID-19 research have rapidly been developed. We asked the individuals behind...is giving Rutgers investigators the agility to rapidly initiate complex research projects that they would...

  2. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... is relatively slow and is not suited to detect rapid changes in DA concentration. To address this, researchers...Studies on the kinetics of these sensors showed rapid response (60 or 130 ms on and 0.7 or 2.5 s off, ... genetically encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish...

  3. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others in clinical...clinical trials (Jin, et al. 2023). In addition to therapeutics, there is a lot of excitement around using sdAbs...Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...Adeno-Associated Virus (AAV) as a Vector for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/...Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https...Vector Production and Transduction. Human Gene Therapy 31:499–511 . https://doi.org/10.1089/hum.2020.069...

  5. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...preclinical and clinical data about a disease or therapeutic Side-by-side comparison of the same experiment...reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology...where the impact of teaching and doing science is rapid, concrete and open to innovation by all.  Additional...

  6. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...thicker peptidoglycan layer, gram positive bacteria trap the crystal violet stain within their membrane, ... added, it dehydrates the peptidoglycan layer, trapping the large dye complex created by the interaction...in marine biology and minoring in studio arts photography.  References and resources References Silhavy...

  7. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...more effectively used in a technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic...synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and... any number of experiments with. The catch The rapid expansion of GCE led to the creation of many new...

  8. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...serotype also has promising therapeutic applications; it can enhance therapeutic delivery across broader ... have identified 180 brain regions. Both this geography and the way the brain is connected to itself across...

  9. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...previously a large barrier for gene therapy. In fact, AAV gene therapies have already been approved in the...Lentivirus Listen to Connie Cepko Discuss Gene Therapy with AAV Figure out How to Get the Most from Your...

  11. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome replication more...types of fluctuations. When cells are dividing rapidly, multiple rounds of replication are initiated before...T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL...

  12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    .... These studies are expected to identify new therapeutic targets/solutions and enable scientists to discover...dominant negative NFIX phenotypes. Developing a rapid test for the functional activity of VUSs will enable...-, NFIB- and NFIC-related human syndromes. As therapies are developed for specific NFI-related genetic...

  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...art with colorful microbes? Try your hand at photography and participate in a science photo competition...Radio, WIRED, Scientific American or National Geographic. Similarly, media fellowships from the British...Indonesia. Since then, I have tried many things: photography, making videos, presenting in pubs and at science...

  14. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...pot.’   Figure 1: Single-Pot Assembly: a) Graphic depiction of chromatin within a nucleus -> DNA ... single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged CRISPR ...

  15. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...type of article. Now, search results display a bar graph of the number of papers with your search term published...several ways to focus your search results. A bar graph of the number of papers with your key term published...blog posts on free tools for making scientific graphics post and social media for scientists ...

  16. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...Vivo Persistence after Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003...Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038...

  17. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...5’-phosphorylated ssDNA guides, which might be rapidly degraded by the cellular machinery, leaving a small...system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise if someone came up...things (101 lessons on Spanish, C++, guitar, photography) and often dreams of a sunny day in Edinburgh...

  18. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... cellulose also efficiently binds, or at least entraps nucleic acids. The Botella lab took advantage of... References Kim, Y., & Morrison, S.L. (2009). A Rapid and Economic In-House DNA Purification Method Using...Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse of Silica Columns from PCR...

  19. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ... the foundation of modern drug discovery and therapeutics. We’ll have more to say about clinical applications...apply this knowledge to create targeted disease therapies with minimal side-effects on healthy cells and... a 3D Fractal Figure 2: DNA compaction. Graphics courtesy of beyondthedish (left) and biobabel ...

  20. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...number of phagemids would be needed for an in vivo therapy. Another strategy for CRISPR antimicrobials combines...replication-competent phages and antibiotics. Phage therapy fell out of fashion with the introduction of antibiotics... phage therapy...
Showing: 101 - 120 of 419 results