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We narrowed to 570 results for: RON

Showing: 201 - 240 of 570 results

  1. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...scientific community.Paratore: I’ve certainly had a front row seat watching the international growth through...whether that be academically or on the employment front. Paratore: I work at Tufts Health Plan and my role...operations. We’re looking for those “what could go wrong scenarios” and making sure we have adequate process...

  2. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...to create a more productive and cohesive team environment. Neglecting communication skillsClear communication... great "baby project," offering a supportive environment to build my management skills. Finally, developing...effectively and foster a cohesive, productive environment. For example, in my current role managing a cross-functional...

  3. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... these conditions. On top of these factors, environmental conditions such as cellular redox state and ...et al, 2012). The third issue relates to the environment of the fluorescent protein. Photostability measurements...in vitro methods allow for a well-controlled environment, but they do not mimic the natural situation....

  4. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ... of a toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during...cell death. ccdA, another gene found in the ccd operon, codes for the antitoxin protein (CcdA) that protects... 6. Tam, JE. and Kline, BC. “Control of the ccd operon in plasmid F.” J Bacteriol. 1989 May;171(5):2353...

  5. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion-based communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural...you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to mind. ...

  6. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...Methods 2018. PubMed PMID: 30377362. Studying neuronal activity using the fluorescent voltage indicator...Listen to the voltage indicator podcast segment The neurons in the brain compute using electrical impulses ... cloning system Article contributed by Michelle Cronin  Listen to the 2in1 cloning podcast segment Recently...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...us, as much as it as about the amazing years in front of us. So in our last celebratory moment, we wanted...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals...Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also started our blog and YouTube...

  8. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing...associated with gRNA introduction (Allen, et al., Frontiers in Genome Editing). This is particularly important...CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021). https://doi.org/...

  9. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...lab mates are coming and going frequently - an environment ripe for valuable work and materials to slip ...think it's worth consideration. Everything will go wrong Whenever an order is placed on the Addgene website...which part of her procedures are the most failure prone. In an academic lab, a similar situation could arise...

  10. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...taken in an industry, regulatory, or clinical environment. It remains contested, however, whether a better...Department at University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral...postdoc-related data collection and analysis. He is also a strong supporter of science Master’s programs. This will...

  11. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer mentors...online content for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs...Plan Learn a Little about The Academic Labor Environment from the Future of Research Symposium Resources...

  12. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...fluorescent proteins, has led to the alternative acronym Fluorescence Resonance Energy Transfer. Unlike ...and acceptor is sensitive to a change in the environment which alters the FRET efficiency. Detecting certain...molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find...

  13. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... precision medicine research. A willingness to confront these tough issues is essential for building a...careers should take advantage of their academic environment and attend seminars, symposia, or courses in ...communication posts Extracurricular activities for a strong science career path Resources on Addgene.org ...

  14. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ... challenge with using AdV is that it triggers a strong immune response after in vivo administration, which...loss of transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes them ideal...identified, re-transform into a bacterial strain not prone to recombination for amplification. The final purified...

  15. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...understand the complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections...genetically encoded fluorescent proteins coupled with Fibronectin intrabodies generated with mRNA display (FingRs...inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous synaptic proteins...

  16. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ... to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor... some in-depth grant writing tips at the Human Frontier Science Program's website. One way to add originality...get some critical insight into what you're doing wrong. All this helps to improve your application for ...

  17. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... morphology, and circuit mapping of a targeted neuronal population.    Figure 2 (modified from...activated and inactivated transgene expression in neurons.  PubMed PMID: 2286602. PubMed Central PMCID: PMC3406316... Michelle Cronin...

  18. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... affinity terminator may be desired for multi-cistronic constructs where high termination efficiency is...Bacterial Transcription Terminators: The RNA 3′-End Chronicles. Journal of molecular biology. 2011. PMID: 21439297...Ther. 2008. PMID: 18627247 Zufferey R, Donello JE, Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional...

  19. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ... 0 and 100 for each guide, with 100 being the strongest interaction between the guide and the target and...interactions (e.g., scores <20) are reduced to 0 and strong interactions (e.g., scores >80) are transformed...weak gRNAs (CFD < 20) bind almost not at all, and strong gRNAs (CFD > 80) bind almost as much as perfect...

  20. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...Compensation beads are a convenient tool to obtain a strong positive signal for a given fluorophore, as they...won’t bind anything (negative population). It is strongly recommended that when you first run a new panel...compensation beads are convenient in that they provide a strong positive signal, even for rare cellular markers...

  21. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...possible because baculoviruses are slightly less prone to recombination than other viruses, like lentivirus.... (2002). Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...produce recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https:...

  22. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...genomic site can be edited based on its chromatin environment. After selecting the best pegRNA design with ...University of Zurich (Switzerland), where he developed a strong passion for computational biology. His expertise...s41587-023-01678-y Li, X., Chen, W., Martin, B. K., Calderon, D., Lee, C., Choi, J., Chardon, F. M., McDiarmid...

  23. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...from two homologous strands of DNA through error-prone non-homologous end joining (NHEJ). In targeted DNA... for cancer therapy. Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan ... https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT...

  24. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...then, using multiple confinement strategies is strongly advised. The effects are multiplicative, and there...technology: because they necessarily alter the shared environment, they must never be used without popular support2...organisms and associated ecosystems.  Because the environment is shared by everyone, he is deeply concerned...

  25. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...constructs were then combined to create a single polycistronic 15 kb insert. The final construct was then introduced...resulting HeLa cells show robust labeling, with strong expression and detection of each fluorophore at...degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation...

  26. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...adenovirus-based gene therapy clinical trial gone horribly wrong. It brought the whole field to a grinding stop and...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...Applications of CRISPR-Based Genome Editing in the Retina. Front Cell Dev Biol. 2018 May 14;6:53. Review. PubMed ...

  27. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...cost-effective, timesaving for transformations. Cons: Prone to satellite colony formation on agar plates, less...less stable at high temperatures and in acidic environments.   Carbenicillin   Carbenicillin, colloquially...antibiotic than amp: it is more stable in high pH environments and is less susceptible to degradation in agar...

  28. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antibiotic resistant cells could repopulate the environment after treatment. The phagemid systems used in...pathogenic or drug-resistant bacteria. It’s a bit ironic that CRISPR, the bacterial immune system, may someday...4. Yosef, Ido, Miriam Manor, Ruth Kiro, and Udi Qimron. “Temperate and lytic bacteriophages programmed...

  29. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work/life balance.... spouse won’t be at every school performance, chaperone every field trip or cheer at every soccer game...birth and breastfeed. Keeping this always at the forefront will go a long way toward alleviating the guilt...

  30. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... and transduces the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced...Ly6a seemed the most likely candidate due to its strong linkage to the PHP.B phenotype and its expression...references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive gene transfer to the ...

  31. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...analysis window shows a green checked circle in front of the sample name. If there was a minor error during... error, you will see a red exclamation point in front of that sample. You can hover over the yellow or...line represents the cut site. Cutting and error-prone repair typically result in mixed sequencing bases...

  33. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living mouse. Image courtesy... and far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021...improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun 5, 3626 (...

  34. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...and Ruddock., 2017), but requires an oxidizing environment to form. When expressed in the non-oxidizing ...cytoplasm. expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm...and screened with in vitro assays to select for strong binders. Then the sequences for the variable domains...

  35. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...adapted for video making.   Cycle One: The Ironing Board Control Center With the pandemic in full ...that included iPad and her laptop raised on an ironing board, giving it the perfect height (Fig. 2). We...to think of something else.   Figure 2: Ironing boards. Good as a WFH desk, and as a video control...

  36. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...research for over a century but was brought to the forefront by Thomas Hunt Morgan, who is known as the “father...work with as they can survive in a variety of environmental conditions, and double every 2 hours. Yeast ...Sydney Brenner brought it into the lab to study neuronal development (Brenner, 1973). C. elegans are small...

  37. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels ...Jonathan. "RNA fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908...

  38. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ... can be from one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such ... A potential solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice...Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503...

  39. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...controls are equally strong, so the normalized value simply reports which POI band is stronger. The right gel...protein in the lane, your loading control won’t be strongly influenced by unexpected variations in any individual...

  40. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...in Xenopus nucleosomes and in complex with its chaperone NPM2, but the technique should be widely applicable...marking cell types in the nervous system By Ashley Waldron The mammalian nervous system is composed of myriad...
Showing: 201 - 240 of 570 results