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We narrowed to 419 results for: RAP

Showing: 121 - 140 of 419 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...lateral flow strips are easy to transport and work rapidly, providing reliable results in as little as an ...diagnostics (e.g. pathogen or virus detection), therapeutics and sensitive genotyping. The beauty of SHERLOCK...within one hour. DETECTR thus has the ability to rapidly detect nucleic acids with high selectivity and ...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...(2024). Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders...interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging with minimal background...2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium...

  3. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...of the sample to that of a standard curve to extrapolate the concentration of antigen in the sample. A... present in the sample and is calculated by extrapolating from the standard curve. Because the primary... present in the sample and is calculated by extrapolating from the standard curve. Scientists often opt...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...delivery and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called...specific cell types. It can also make CAR-T cell therapy more accessible by simplifying the production process... offers a promising approach for experimental therapies and medical applications.  Find DIRECTED plasmids...

  5. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...vector particles.     Figure 3: Electron micrograph of AAV vector preparation shows that the vast...genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089...a Novel Droplet Digital PCR Method. Human Gene Therapy Methods, 29(2), 96–103. https://doi.org/10.1089...

  6. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...closing. Parts of a cover letter The opening paragraph is the place to introduce yourself and your background...body of the cover letter should be one to three paragraphs. This is where you make the case for why you ...questions the hiring manager may have. The closing paragraph should again show your enthusiasm for getting ...

  7. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...Cells Are Inactivated by Human Serum. Molecular Therapy 2:218–222 . https://doi.org/10.1006/mthe.2000.0116...Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org/10.3390/v12091016...Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089/hgtb...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...other large research institutions. The following graphic depicts the ways in which the two guiding principles...it difficult or impossible to reproduce. As the graphic depicts, RSpace supports linking to all of the ... other tools and resources. As depicted in the graphic, RSpace is integrated with most of the tools noted...

  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...membrane permeable and non toxic to cells. Because it rapidly photobleaches, DFHBI can only be used for qualitative...25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic of green fluorescent protein...PubMed Central PMCID: PMC3775879. Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin. "Development...

  10. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...pitch that requires reading two pages or even a paragraph to get to the punch line is a terrible sales pitch...conviction that I had a flair for communication was rapidly dispelled. She would ask me, "Is this what you ...that we can manipulate to study pathology and therapies." This is only the first few minutes of the...

  11. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... decomposition analysis is performed yielding a graph of edits in the input population or clone.   ...sgRNA sequence. The TIDE software will generate a graph representing all insertions and deletions identified...MC, Bombieri N, Giugno R, Pinello L. CRISPRitz: rapid, high-throughput and variant-aware in silico off-target...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...are easy to see (and for heavens sakes, label all graphs and data professionally). Do remember the fine ... use no notes and can concentrate on delivery, rapport with the audience, and their own excitement about...

  13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...Regions of the Central Nervous System. Molecular Therapy 10:302–317. https://doi.org/10.1016/j.ymthe.2004.05.024...Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678. https://doi.org/10.1089/hum.2019.277...Peripheral Nervous System and Relevance to Human Gene Therapy. Front Mol Neurosci 13. https://doi.org/10.3389...

  14. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...the culture. This system is now used as it shows rapid gene expression kinetics as compared to the Tet-...Regulatable Lentiviral Vector System, Viral Gene Therapy, Dr. Ke Xu (Ed.), ISBN: 978-953-307-539-6, InTech...(2000): 249-71. PubMed PMID: 10983732. 5. Gene Therapy Net Lentiviral Vectors Page Additional Resources...

  15. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...translational researchers begin to use CRISPR as a therapy. Low frequency off-target effects may generate ...play significantly into the realm of clinical therapeutic development as CRISPR is used not only to study...CRISPR/Cas9, and megaTAL nucleases." Molecular Therapy (2015). PubMed PMID: 26502778. 13. Frock, Richard...

  16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently become...Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural...Tags Fluorescent tagging of endogenous genes with SapTrap Resources on Addgene.org Browse CRISPR plasmids...

  17. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...University. No matter whether you are a sports photographer at the Super Bowl, a medical technologist taking...just those in the focal volume. This leads to more rapid photobleaching and a reduction in signal intensity... 2-photon microscopy, lightsheet microscopy is rapidly becoming the preferred technique for many of these...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...2014.   In vivo uses For both research and therapeutic purposes, antibodies can be used to affect cellular...functional grade antibodies. Some of the research and therapeutic uses overlap: antibodies can bind to and inhibit...investigating signaling pathways or developing therapeutics. Experimentally, antibodies conjugated to fluorescent...

  19. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ... before it, offers an interesting view into how rapid innovation affects the research community. I’ve ... not. It is an offline tool, but comes with a graphical user interface. Best strategy for labs: Define...believe the future of CRISPR software is promising. Rapid innovation in this space has put us in a good position...

  20. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...probe into a single molecule often work better for rapid-cycle PCR, such as the LightCycler, as it reduces...chemistry, unless running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr ...brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler, you’ll likely...
Showing: 121 - 140 of 419 results