Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 570 results for: RON

Showing: 241 - 280 of 570 results

  1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...plasma HIV-1, they are at increased risk for other chronic diseases, including dementia, gut disorders, nerve...disease risk is attributed to latent HIV reservoirs, chronic inflammation, and negative metabolic effects of...in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR...

  2. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed... fhuA (formerly tonA) ferric hydroxamate uptake, iron uptake receptor mutation. T1/T5 Phage resistance...not degraded, cell cannot methylate DNA lac Lac operon mutations Blue/white screening of clones   lacIq...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... was engineered from the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by...displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it...sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128...

  4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...confidence that they aren’t simply doing an experiment wrong. However, even simple procedures like pouring a ... easier to learn new experimental techniques. Electronic lab notebooks also play a role here by making...scientists with positive results have done nothing wrong, but the problem is that only these positive results...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...from Yang et al. requires two gRNAs targeting two intronic regions and two repair templates in order to insert... allele requires you to target 2 loxP sites to intronic regions 0.5 - 0.8 kb apart. In the example below...CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer ...

  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ... E. coli or Salmonella, or any cells that bind strongly to nitrocellulose, over-incubation of plates can...pathways for bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016). PubMed PMID: 27536293...genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649-1652. PubMed ...

  7. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...very hard to speak with individuals who had very strong beliefs in traditional medicine, especially when... reliant on programming skills which are not my strong point. Working in industry: virology On completing...because it is an English speaking country with a strong economy and plenty of jobs in science at world ...

  8. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...co-workers who want to make our organization as environmentally friendly as possible. We worked with Andrew... unless we manage to make Addgene the most environmentally friendly organization on the planet, in which...easier. Trust Ultimately, your team could have a strong sense of purpose, an excellent leader, and perfectly...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...carrier of genetic information and led to the coronation of DNA as the molecule of life.  This was further...genetic engineering experiment which goes horribly wrong. The plot of the movie involves The Rock and his....111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering with CRISPR-Cas9. Science...

  10. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...e.g. an R01 from the NIH, in the United States) Strong letters of reference that attest to your talent...person makes time for you, as needed, this is a strong indicator that this person cares about your science... to finding out you've been doing something all wrong or that a reagent has not been convincingly demonstrated...

  11. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...and how do they come off and on in a cellular environment? If these are pressing issues in your laboratory...majority of FRET acceptors are delivered to the wrong address in a different city, you can still develop...list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors, absolute FRET acceptor...

  13. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid...protocols and information, be sure to check out the Trono Lab website. If you have plasmids you’d like to ...

  14. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...around the world!) that have kept Addgene at the forefront of life sciences research for the past decade....not just for a better day at the office but for stronger science as well. This blog is our way of continuing...

  15. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...spatial, cellular, metabolic, and cytokine microenvironment that exists in vivo. Over the past five years...cell type, in more complex and physiological microenvironments. These screens have primarily been performed...

  17. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...spatially with Cre-recombination. Read the paper in Frontiers in Molecular Neuroscience Find the AAV-based...can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration...

  18. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ...they have a graduate degree in science. I find it ironic that we do all of our training as scientists (5...jobs (that is what networking is about), but be upfront about interest in a job. Ask openly to be connected...

  20. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...liberated to adorn the walls of universities and the front pages of newspapers. Science is now being adapted...while - a tool to expand perception, which is ironically what science is about. For me it is essential...

  21. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...results in the degeneration of spinal cord motor neurons. This disease is estimated to affect anywhere from...for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy patients. Noushin...

  22. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...increasingly accessing websites other than through the ‘front door,’ the homepage is still important. It needs...easily. In the redesign, the new search bar is now front and center, with a clear call to action - Search...

  23. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...important to this function. With the welcoming environment on my team and at Addgene, I felt comfortable...seemed. The transition into a traditional work environment was aided by the efforts being put in by Addgene...

  24. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ... tobacco, Arabidopsis, and corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple...Transgenesis with GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids...

  25. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...test recombination efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent...hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows...

  26. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...vehemently about the need for change.  Dr. Bourne strongly believes this is an amazing time of promise for... Novartis industry postdoctoral program.  His strongest comments were about the astounding lack of reproducibility...

  28. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...Structure and Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb...

  29. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...splice acceptors. They require insertion into an intron of a gene, where they can be spliced into the gene...

  30. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...that there are many ways your experiments can go wrong. Whether it be a bad fridge freezing your samples...editing and later looking for songs and sounds to synchronize with the images thereby making the videos more...

  31. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic...parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll cover the common ...

  32. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...Antibodies: Historical Perspective and Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589... Ashley Waldron...

  33. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...of input material, cells or tissue, to produce a strong enough signal over background noise. This is not...protocol, the most significant being the usage of a strong detergent to permeabilize cells rather than to ...

  34. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...It is probably good news for our environment that more people are paying attention to living a “green...reagents and buffers.  However, I had very environmentally conscious friends who had me thinking about...

  35. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...possible option, we place the most important controls front and center, and make specialized controls available...plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we dug into the original...

  36. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...with ever more efficient tools for light mediated neuronal control and we are expanding our viral service...photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from...

  39. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...allowed to percolate within a new intellectual environment. Over time, this interest came to incorporate...
Showing: 241 - 280 of 570 results