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We narrowed to 570 results for: RON

Showing: 281 - 320 of 570 results

  1. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ...

  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...elegans, they inserted the SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG...resulting loxP scar is located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag...

  3. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...high GC content and hairpin structures, ITRs are prone to replication errors and disruptive mutations and...improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing frameshift knockout...Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389/fmicb.2020.607028...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...CRISPRainbow. Both tools were targeted to the FBN3 intronic repeat which contains 22 copies of the gRNA target...distances of loci pairs from intergenic regions, intronic regions, and a pericentromeric region. All pairs...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found.... Exo-AAV9 was successfully shown to transduce neurons in the brain after systemic (i.v.) delivery in ...

  7. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently join...-executed cloning endeavor, NEBuilder requires upfront planning. This cloning methodology requires that...

  11. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...shipped), where they would be exposed to a closed environment containing CO2 (from the dry ice). After 48 hours...PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M,...

  12. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...10.5%  3.2  534.1 3 2010 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010... 2010 167  Applied and Environmental Microbiology  1.6% 1.2%  0.75   125.3 In figure 2, you can...

  13. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...disease mutation in basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. 2018; see ... Limitations, and Implications for the Future.” Front. Oncol. https://doi.org/10.3389/fonc.2020.01387 ...

  14. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...number, but it was attained without any of the error-prone repair that normally accompanies Cas9 cutting. We... holds some kind of fitness advantage but error-prone repair of the gene would be disastrous (i.e. if ...

  15. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...effective delegation skills: The team will be stronger and less dependent on the manager to succeed. ... your team and take responsibility if things go wrong. Here are some important criteria for delegating...

  16. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...immunogenicity, which means they do not stimulate a strong immune response when introduced into an organism...Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et al. “Altering the tropism of lentiviral vectors...

  19. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a useful tool...genes to specific cell types in the brain, mapping neuronal networks, and selectively activating signaling...

  21. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... use amplicons in ITR as we have found this to strongly bias titer. We are also adopting ddPCR, which ...microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and...

  22. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann ...doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced...

  23. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...make a knockout in your gene of interest, as it is prone to generating indel errors. Indel errors generated...Non-Homologous End-Joining Really an Inherently Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org...

  24. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...themselves if they want this beautiful flower in their front yard or not. By developing plant aesthetics with...

  26. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...disease in question – cardiomyocytes, adipocytes or neurons, for instance – to see how those mutations influence...TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing system for ...

  27. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ... means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or ... Ashley Waldron...

  28. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...particularly helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References...

  29. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...telomeres and centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to...

  30. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ... psoriasis Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect...genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes...

  31. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did...

  34. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...for endocrine steroids (i.e., corticosteroids, progesterone, androgens, and estrogens), fat-soluble vitamins... the genome to sense the impact of the total environment on the cell. The NURSA Consortium Now in its ...

  35. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to...will convince the reviewers that the proposal is strong, well-thought out, and will be successful when ...

  36. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...effective as it can result in the retention of introns or the removal of exons. Disrupting promoter or... Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. doi:10.3389/fpls.2015.01001 Ho...

  37. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...isn’t too different, consider antibody affinity: stronger binding antibodies will require harsher stripping...depend on your blot — antibodies that bind more strongly or that have saturated the blot may need longer...

  38. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ... fidelity, while the 52-piece assembly of a lac operon dropped to 49% fidelity, with significant lot-to-lot...Assembly, a great place to start is the 24-piece lac operon assembly deposited by NEB – and don’t forget to...

  39. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...sites on the target protein they tend to have a strong signal and to work in a variety of applications...Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283...

  40. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...started out as a small collection of plasmids for coronavirus research, including a handful of plasmids that... Find COVID-19 resources on our COVID-19 and Coronavirus Plasmids & Resources page Stay up-to-date on ...
Showing: 281 - 320 of 570 results