Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 419 results for: RAP

Showing: 161 - 180 of 419 results

  1. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ... mixture on solid media. The colonies are then scraped off the plates and pelleted for DNA extraction....amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped out, ...

  3. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...issues raised around mitochondrial replacement therapies. In other words, rather than having a meeting...to tackle a controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR...

  5. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...figure below). Subsequent exposure to red light rapidly deactivates the system. Voigt’s lab used this system...GFP reporter. Subsequent exposure to red light rapidly deactivates the system. Version 1 of this far-red...

  6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and... GEmCherry2 reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency...

  8. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses. Students...Students then use cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech...

  10. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...AAV has many excellent features for human gene therapy and is currently being used in clinical settings...Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes...

  11. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...it, marking it as GFP+. Quantification for such graphs could simply be the percentage of the total cells...background in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...region of the 3’ LTR and are primarily used in gene therapy research to reduce promoter interference and/or...References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient gene delivery...

  13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...could greatly enhance our ability towards CTD therapeutic development,” says Iyer. Since the RESOLUTE plasmid...says Mellett. This would help the lab identify therapeutic targets in the future. Congratulations Mark Mellett...

  14. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ... isolated from a complex mix using affinity chromatography against an amino acid sequence, or tag, on ...Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient purification of native histidine-tagged...

  15. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...what if we had a simple tool that allowed us to rapidly decode the complexity? The CombiGEM-CRISPR technology...for genome editing in large-scale has led to the rapid generation of genome-wide gRNA libraries that knock...

  16. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ... discovery and the development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al...Studying clonal dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine...

  17. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...section, contact information, and potentially a photograph of yourself. A photo can be very useful in the.... Shapes. In this type of poster, written and graphical information are inserted into larger circles or...

  18. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...these tough issues is essential for building a rapport with these communities, which is in turn crucial...plans, or information briefs for policymakers. The rapidly changing landscape of genetics and our consistent...

  19. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo AG, Gautier... for chromophore maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag...

  20. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... basic biological research as well as clinical therapy. These biological tools are useful because they...involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common...
Showing: 161 - 180 of 419 results