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We narrowed to 570 results for: RON

Showing: 321 - 360 of 570 results

  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral ...channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons is now available as a ready to use viral prep...

  2. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it? Please address as soon ... If the customer service rep does not tell you upfront how long it will take to get an answer, consider...

  3. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...solution that has been thawed more than once. Fibronectin is another membrane-interacting protein that ... 7. Stockschläder, M., et al. "Expansion and fibronectin-enhanced retroviral transduction of primary human...

  4. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...scientists can get involved in modelling, the electronics folks can build a robot worm on top of that model...final target decisions, we intend to focus on motor neuron knockouts so that we can perturb factors related...

  5. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ... meet your supervisor’s expectations is to ask upfront: “How frequently would you like me to check in ...supervisor’s suggestions and take your project in the wrong direction. The best way to avoid miscommunication...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...developing a new fluorescent protein tool that responds strongly to magnetic fields (Frank Hayward et al., 2024...science in the open The York Lab operates with a strong commitment to exploration and collaboration. Andrew...

  7. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ... library in a lentiviral backbone might be more prone to recombination, while a library with a large backbone...transcription factor overexpression library) or is prone to recombination, we recommend amplification on ...

  8. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all the things! ... Ashley Waldron...

  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...apart is the way they generate their color: they strongly absorb visible light to give colors in ambient...Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16). https://doi.org/10.1128...

  10. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...What if a tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck into the prep during... References and resources References Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence for Encapsidation...

  12. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...researchers have realized how problematic aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...

  13. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...the resistance cassette has been placed into an intron.    Figure 4: A targeting vector to create... Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed. You'd...

  14. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...career path. Luckily, in grad school much of the up front work was done for me. I just had to take advantage...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

  15. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...repair. NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In...plasmid integration; however, since NHEJ is error-prone, such a system is likely to introduce additional...

  16. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... target DNA, which, when repaired through error-prone non-homologous end joining (NHEJ), usually results...of multiple gRNAs targeting the same gene can be strong evidence that a particular gene is actually important...

  17. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ... you have multiple contaminating bands or a few strong ones, consider increasing the number of washes ...Schirrmann T. Expression of recombinant antibodies. Front Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217...

  18. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...exposure, a lack of immunity, and then finally a synchronized exposure under these conditions once the measures...proteins and downstream cellular events, all recruit strong innate and adaptive immune responses that inactivate...

  19. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy to understand...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...

  21. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...across most, if not all, industries and work environments. This is why they are called transferable skills...leader,” because you don’t see yourself  standing in front of a crowd and using your charisma to give people...

  22. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... of science, particularly public health and environmental science, by the Trump administration are difficulties... climate change, the degradation of the U.S. environment, and a decline in the competitiveness of U.S....

  24. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...can occur in two different orientations, but is strongly biased towards T-RL, where the R end is proximal...https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg...

  26. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...to this question.  We truly work in a unique environment where, in addition to furthering our mission ...). 3. Do something outside of your ordinary environment Team building events like Escape the Room or ...

  27. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models... a particular area, e.g. education, poverty, environment, early childhood development, or community development...

  29. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage explains how lentiviral particles are... of this article. Additional Resources: Didier Trono Lab: Lentivectors Toolbox Resources on the Addgene...

  30. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Epitope tag antibody collection grows By Ashley Waldron We are excited to highlight the Anti-V5 tag antibody...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs...

  31. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter is...job with a lot of file management that asks for strong organizational skills. You might think, “I’d be...

  32. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...visible under both phase contrast microscopy and electron microscopy (Fig. 1b). Gas vesicle expression ...batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression and robustness without chromatin-opening...

  33. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...development and evolution of histone genes in Strongylocentrotus purpuratus, the spiny purple sea urchin. ...demonstrate unusual parallel adaptation to disparate environments so can be used study the genetic basis of evolution...

  34. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training... until I was out of the lab and in an office environment. Ultimately, it will likely be these skills that...

  35. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...

  37. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...the chance of the insert being “dropped” in the wrong place, while increasing the number of potential ...

  38. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...bacterium picking up resistance genes from it’s environment (from DNA left behind after another bacterium...survival. Existing virtually ubiquitously in the environment, there is an intense amount of competition between...

  39. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ... the most rewarding parts of the job is being confronted with challenges that are crucial for the success...in a short amount of time can sometimes become cauldrons on the verge of boiling. On other occasions, clients...

  40. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...scientists with the ability to selectively silence neurons in vivo (Slimko and Lester, 2003).  Addgene has...
Showing: 321 - 360 of 570 results