We narrowed to 514 results for: CIT

...moments. The models function as thinking tools – eliciting questions like “what does this model representt...proteins for researchers. Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if ...in a rationally engineered Cas9 with improved specificity. Hold this flexible model in your hand for a ...

...light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...

...history was very much in the making. One of the exciting, and challenging, aspects of talking to 8th graders... proficient in science, regardless of race or ethnicity. Perhaps you don’t have children but are interested...strawberry, or demonstrated that they’re living in an exciting, tumultuous time in scientific history. Whatever...

...application and submitting it, to evaluating the capacity of an organization to receive and manage funds... was moved from one department to another within city government. Throughout these moves and program changes... our program didn’t stay in one place within the city infrastructure. My career path changed when I was...

...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...programs for K-12 students. You can see why I was excited to be invited to attend. My job is to ensure that...marmorated stink bugs to make them pink. We’re excited to see new tools being developed and shared for...

...or “Do you have additional data regarding the specificity of this antibody?” You will also help troubleshoot... customers everyday, it can be fascinating and exciting to listen to the many individual stories and get... of science communication, and is particularly excited to hear about novel developments in the fields ...

... however, either lack the sensitivity or the specificity to detect nucleic acids at low concentrations...atomolar sensitivity and single base pair mismatch specificity.  SHERLOCKv2 SHERLOCK had a few limitations when...detect nucleic acids with high sensitivity and specificity without compromising speed, ease of use, and ...

...plasmids, optimized for a variety of species.   Specificity concerns? If off-target effects are a worry, ...nickases (Cas9n) to the target locus, enhancing specificity over a single SpCas9. The drawback of this approach...and consider using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9...

...targeting of specific cell types, it has a limited capacity to carry large DNA cargos. As a result, the search...focus in recent years. TnpB nucleases represent an exciting class of compact genome editors, typically only...limitation of viral vectors is their restricted cargo capacity, which makes it difficult to accommodate the large...

...single molecules of an RNA species with high specificity (Gootenberg et al., 2017). This system, dubbed...Cas13a in mammalian cells The Zhang lab made the exciting discovery that some Cas13a orthologs can function...Cas13b as a tool for RNA base editing,, and we are excited to see this group of enzymes continue to make a...

...something that could move your project onto the exciting—and unanticipated—road of discovery. Monitoring...inhibition), and behavioral (remote memory retrieval) specificity. Optogenetics can be used to more precisely associate....    References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala to nucleus accumbens...

... appropriate as they allow you to assess the specificity of your observed result. Non-targeting controls...important reference point when analyzing the specificity of the shRNA targeting human Gene X. The non-...describe these a bit more below, but the papers cited in the reference section provide more in depth information...

...make it a great viral vector, but its packaging capacity is limited to ~4.7kb, or roughly half the packaging...Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus Capsids. Human Gene ...Expands Adeno-associated Viral Vector Packaging Capacity in a Transgene-independent Manner. Molecular Therapy...

...government can be defined as a social contract citizens enter into in order to protect their rights (2...and often funds [3]) scientists as well as other citizens. Thus civilization proceeds in an informed fashion...political action, and inspires politicians and citizens to include science in decision making, it will...

...severing and has very low dark-state activity and toxicity. All of that means this light-powered sword truly... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks interested in ...

... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...about how the sharing model works. See if you can elicit helpful comments from your audience as in “Does...delivery, rapport with the audience, and their own excitement about the topic. Don’t be fooled by thinking ...

...easier to share data through their curation of citable, linkable figures. Cause #2: Hidden “negative” ... #3: Poor experimental design It’s easy to get excited about positive results. Not only is it scientifically...academic research). Initial positive results and the excitement they generate can, however, bias us. We’ve all...

...popular due to its durability and high binding capacity. In addition, PVDF membranes can be repeatedly...times. If the vendor includes publications that cited the antibody, consider reviewing the materials and...with antisera: a method for studying antibody specificity and antigen structure. Proceedings of the National...

...because of its relative simplicity as a gene editing system. Despite that simplicity, its accessibility outside...transfer increases the metabolic and metastatic capacity of cancer cells. This is certainly just the beginning...

... Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI). Anti-vinculin Western blotting...shifting landscape of research opportunities, it’s an exciting time to be in the lab. Based on the power of available...easy it is to share information and regents, it’s exciting to think that no matter who you are, you could...