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We narrowed to 504 results for: CIT

Showing: 161 - 180 of 504 results

  1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...programs for K-12 students. You can see why I was excited to be invited to attend. My job is to ensure that...marmorated stink bugs to make them pink. We’re excited to see new tools being developed and shared for...

  2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...single molecules of an RNA species with high specificity (Gootenberg et al., 2017). This system, dubbed...Cas13a in mammalian cells The Zhang lab made the exciting discovery that some Cas13a orthologs can function...Cas13b as a tool for RNA base editing,, and we are excited to see this group of enzymes continue to make a...

  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...plasmids, optimized for a variety of species.   Specificity concerns? If off-target effects are a worry, ...nickases (Cas9n) to the target locus, enhancing specificity over a single SpCas9. The drawback of this approach...and consider using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9...

  4. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...something that could move your project onto the exciting—and unanticipated—road of discovery. Monitoring...inhibition), and behavioral (remote memory retrieval) specificity. Optogenetics can be used to more precisely associate....    References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala to nucleus accumbens...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ... appropriate as they allow you to assess the specificity of your observed result. Non-targeting controls...important reference point when analyzing the specificity of the shRNA targeting human Gene X. The non-...describe these a bit more below, but the papers cited in the reference section provide more in depth information...

  6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...make it a great viral vector, but its packaging capacity is limited to ~4.7kb, or roughly half the packaging...Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus Capsids. Human Gene ...Expands Adeno-associated Viral Vector Packaging Capacity in a Transgene-independent Manner. Molecular Therapy...

  7. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...government can be defined as a social contract citizens enter into in order to protect their rights (2...and often funds [3]) scientists as well as other citizens. Thus civilization proceeds in an informed fashion...political action, and inspires politicians and citizens to include science in decision making, it will...

  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...severing and has very low dark-state activity and toxicity. All of that means this light-powered sword truly... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks interested in ...

  9. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...about how the sharing model works. See if you can elicit helpful comments from your audience as in “Does...delivery, rapport with the audience, and their own excitement about the topic. Don’t be fooled by thinking ...

  10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...easier to share data through their curation of citable, linkable figures. Cause #2: Hidden “negative” ... #3: Poor experimental design It’s easy to get excited about positive results. Not only is it scientifically...academic research). Initial positive results and the excitement they generate can, however, bias us. We’ve all...

  11. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...popular due to its durability and high binding capacity. In addition, PVDF membranes can be repeatedly...times. If the vendor includes publications that cited the antibody, consider reviewing the materials and...with antisera: a method for studying antibody specificity and antigen structure. Proceedings of the National...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...read many base pairs simultaneously to increase specificity. This modular approach made ZFNs highly appealing...individual nucleotides. This imparts TALENs more specificity and a broader substrate preference than the ZFNs...Seligman LM (2002) Mutations altering the cleavage specificity of a homing endonuclease. Nucleic Acids Research...

  13. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...Nature Education 1(1):204. https://www.nature.com/scitable/topicpage/transposons-the-jumping-genes-518/   ...

  14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI). Anti-vinculin Western blotting...shifting landscape of research opportunities, it’s an exciting time to be in the lab. Based on the power of available...easy it is to share information and regents, it’s exciting to think that no matter who you are, you could...

  15. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...your experiment. They come in all sizes and specificities, from target-specific ones to application-specific...Search engine May include validation data CiteAb Any Any Search engine May include validation...

  16. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...want to hear and see even more of us? We’re very excited to be launching the official Addgene TikTok at ...of shenanigans we get into!  We hope you are as excited as we are! Come join us in our blog, video, and...

  17. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...days away and here at Addgene we couldn’t be more excited! As a small, tight-knit organization we thrive ...the coming days. Hopefully, I’ve made clear how excited we get for Halloween and how seriously we take ...

  18. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...hours? A colossal 547 years! While we are very excited about reaching this milestone, it wouldn’t have...their contributions on social media as well the excitement amongst our Addgenies on social media or around...

  20. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...Lianna Swanson was very popular, with the students excited to see a real working lab full of busy Addgenies...toothpick was very cool!” and Izzy Shaw, who was excited about “Seeing a real lab!” And several of our visitors...
Showing: 161 - 180 of 504 results