...still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com... CRISPR-Cas and Cascade for large genomic deletions. CRISPR...comprised of a combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade is directed to target DNA using... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3 Browse ...recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling DNA using its helicase properties...this, some CRISPR-Cas3 plasmids take an all-in-one approach and include Cas3 and Cascade on one single plasmid...Blog Posts Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates ...

...Nucleases (RFNs) CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids...about RGNs and CRISPR-Cas systems can also be found at http://www.crispr-cas.org . Users are also encouraged...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...Plasmids Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR...RGNs ), based on naturally occurring Type II CRISPR-Cas systems, are programmable endonucleases that can ... to additional pages describing Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression plasmids (AsCpf1 and...specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered PAM...

...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...Drosophila and other insects. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB... Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49410...

...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...

...prevent the CRISPR-Cas complex from binding to DNA. Others inhibit the recruitment of Cas proteins to the...allowing multiple Cas9s to be used together. Researchers have engineered mutant Cas enzymes with alternative... Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using ...immune system - utilizing various CRISPR-associated (Cas) genes to not only store a record of invading phages...upon re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci... Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material...

...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759...none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

...two main classes of CASTs are type I and type V-K. Type I systems use a type I Cas effector, composed ...composed of several separate Cas enzymes that form a complex called Cascade ( C RISPR- as sociated c omplex for...matters. Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB,...transposases (Tns) with the precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large fragments...fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead recruiting the... CRISPR CASTs CRISPR Plasmids: CRISPR Transposases (CASTs) Browse...transposases (CASTs) for insertion of donor DNA. CRISPR...

...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty ...need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid... none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem...

...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...for use in bacteria. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB.... ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...

...type I Cas effector, composed of several separate Cas enzymes that form a complex called Cascade (see Cas3...Base Editing with CRISPR CasPEDIA: A Functional Classification of Cas Enzymes Cas Enzymes and PAM Sequences...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...editing with Cas3 Browse Plasmids: Cascade-Cas3 Want more information on the wide variety of Cas enzymes? ...from. Many variations of Cas9 exist, along with other Cas enzymes like Cas12a, which are available for...paired with the appropriate Cas enzyme. Specific enzymes, such as Cas12a or Cas13 , can improve multiplexing...Other natural Cas orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and Nme2Cas9 (from Neisseria...