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We narrowed to 97 results for: REN

Showing: 1 - 10 of 97 results
  1. CRISPR References and Information

    Type
    Collection
    ...and references, including gRNA design tools, CRISPR FAQs, and community forums.... CRISPR References and ...and Information CRISPR References and Information Addgene Resources CRISPR Software General Resources CRISPR...kits. CRISPR Software Sanger Indel Analysis ICE (Inference of CRISPR Edits) This open access tool from Synthego...automate and optimize gRNA and HDR donor design. The current version supports SpCas9, SpCas9-VQR, and enAsCas12a...opens in a new window) This tool helps design (10 different prediction scores), clone (primer design), and...inactivation, NHEJ, and HDR. Includes a wide range of reference genomes, including animals, plants, bacteria, ...
  2. Luciferase Plasmid Collection

    Type
    Collection
    ...Taipale 87065 pcDNA3.1-ccdB-Renilla Renilla Creation of C-terminal Renilla luciferase fusions using Gateway...Mikko Taipale 87068 pcDNA3.1-Renilla-ccdB Renilla Creation of N-terminal Renilla luciferase fusions using ...74444 pLenti.PGK.blast-Renilla_Luciferase Renilla PGK Lentiviral expression of renilla luciferase Reuben Shaw...Root 118016 pLX313-Renilla luciferase Renilla EF1α Lentiviral expression of renilla luciferase William...luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral Expression of renilla luciferase...Diego Orzaez 68215 pEGB 35S:Renilla:Tnos (GB0109) Renilla 35S Expression of Renilla luciferase in plants ...12179 pIS1 Renilla Insertion of target sequence in the 3' UTR David Bartel 12177 pIS2 Renilla Insertion...
  3. Plasmids for Stem Cell Research

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    Collection
    ...Research Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids...unspecialized cells with the capacity to differentiate into many different cell types in the body during development...can be directly differentiated into specific somatic stem cells or fully differentiated cell types. Browse...collection of Differentiation Plasmids below. Alternatively, it is also possible to directly differentiate one ...one differentiated somatic cell into another via transdifferentiation. This method of cell lineage conversion... of Transdifferentiation Plasmids below. Many of the techniques for reprogramming, differentiation, and... , SOX2 , MYC , KLF4 , LIN28 . Differentiation and Transdifferentiation Plasmids Browse the table below...
  4. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Biosensor Loren Looger AV-1-PV2724 98931-AAV1 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Biosensor Loren Looger AV-5-PV2724 98931-AAV5 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Biosensor Loren Looger AV-9-PV2724 98931-AAV9 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Control Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren Looger ....2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen AV-1-PV3509 98925-AAV1 pAAV.CAG.Ruby2sm-Flag.WPRE.SV40 Loren Looger AV-1-PV3854... list of Addgene's viral vectors See Addgene's Current AAV inventory , which includes all viral vectors...AAV1 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2816 100835-AAV1 pAAV.CAG.Flex.GCaMP6f.WPRE.SV40...
  5. Antibody Guide

    Type
    Collection
    ...heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and ...of colors activated by different wavelengths, allowing for easy differentiation between multiple antibodies...roughly fifty different fluorophore colors in flow cytometry, but the largest panels used currently are in the...Antibodies are typically produced in one of three different methods: in animals, in cultured immune cells,...contain two Fab fragments, each recognizing a different epitope, bound by short peptide linkers. Diabodies...used as an antimicrobial agent. Other chemical interferences with conjugating reactions are possible. It ...chemiluminescent reaction in the presence of several different substrates. The luminescence produced by the reaction...
  6. CRISPR Guide

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    ...created using BioRender.com (Link opens in a new window) . Addgene Website & Blog References Website CRISPR..., A., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024). Current strategies...Alternatives Resources Plan Your Experiment Web References PAM Sequences Glossary Publications CRISPR Overview...mutation within the targeted gene. However, the strength of the knockout phenotype for a given mutant cell... into high fidelity Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes they...pyrimidine-to-pyrimidine) Base transversions - edit to different classification of nucleotide base (purine-to-pyrimidine...genetic changes, including moving entire genes to different locations, by combining other gene editing mechanisms...
  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PAQR2 ADM adrenomedullin AM ADM2 adrenomedullin 2 AM2, FLJ21135, dJ579N16.4 ADRB1 adrenergic, beta-1-,... MGC138246 GDF1 growth differentiation factor 1 - GDF10 growth differentiation factor 10 BMP-3b, BMP3B...BMP3B GDF11 growth differentiation factor 11 BMP-11, BMP11 GDF15 growth differentiation factor 15 GDF-15,... PTGFB GDF2 growth differentiation factor 2 BMP-9, BMP9 GDF3 growth differentiation factor 3 - GDF5 growth...growth differentiation factor 5 BMP14, CDMP1, LAP4, OS5, SYNS2 GDF6 growth differentiation factor 6 BMP13...MGC158101, SGM1 GDF7 growth differentiation factor 7 BMP12 GDF9 growth differentiation factor 9 - GDNF glial...PPH1, T-ALK GDF1 growth differentiation factor 1 - GDF10 growth differentiation factor 10 BMP-3b, BMP3B...
  8. Rett Syndrome

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    Collection
    ...information, prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal and adult...approximately 1:10,000. It is characterized by apparently normal development through the first 6 months... skills occurs. Diagnosis of Rett syndrome is currently based on specific clinical diagnostic criteria... known to cause Rett syndrome, however, eight recurrent missense and nonsense mutations (R106W, R133C,...system for generating Rett syndrome-specific differentiated cells that can be used to understand the mechanisms...immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific...(Link opens in a new window) Rettsyndrome.org References Amir et al. 1999. Rett syndrome is caused by ...
  9. Cre-lox system

    Type
    Collection
    ...yeast Gal1 Yeast Sandmeyer 26888 pmCherry-CRY2-CreN CRY2-CreN and mCherry coexpression; Light inducible; ...Constructs Cre Reporters & Tools Additional Resources References Background The Cre-lox system, derived from P1...respectively) and placed under the control of different promoters. Expression of both N and CCre in the...high-titer viral preparations of plasmids that are currently in our repository. ID Plasmid Description Promoter... Roxed-Cre GFAP Mammalian Pelczar 51267 pCAG-Co-InCreN N-terminal component of the Co-InCre system CAG... cre recombinase CAG AAV Bruchas 75268 pAAV-CRY2-CreN photo-activatable cre recombinase CAG AAV Bruchas...Cre-pA Cre; for atrial-specific expression ANF AAV Wehrens 121675 pAAV-EF1a-fDIO-Cre Cre EF-1 alpha AAV Engel...
  10. Fluorescent Protein Guide: Biosensors

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    Collection
    ...for a different analyte, a sensor for gene-specific activity, or for popular biosensors in different expression...the articles linked below include variants with different binding affinities or negative-control constructs...Cre-dependent) Cell-specific restoration of stimulus preference after monocular deprivation in the visual cortex...3;32(40):13819-40. Baljit Khakh , Douglas Kim , Loren Looger , GENIE Project Calcium GCaMP3 genetically...indicators. Nat Methods. 2009 Dec;6(12):875-81. Loren Looger Calcium NEMO calcium sensors Engineering ...integrators. Science. 2015 Feb 13;347(6223):755-60. Loren Looger , Eric Schreiter Calcium Intersectional viral...cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP in vascular smooth muscle...
Showing: 1 - 10 of 97 results