We narrowed to 24 results for: Coli

...modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli Strains for Protein Expression Protein...aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic Esposito...Arabinose Escherichia coli Christian Raetz 26098 pCW-LIC 3xPtac Lactose/IPTG Escherichia coli Cheryl Arrowsmith...Escherichia coli Cheryl Arrowsmith 43795 pDusk pR_FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich.../IPTG Escherichia coli Cheryl Arrowsmith 46886 pMSP3535 PnisA Nisin Escherichia coli , gram-positive bacteria...Escherichia coli , Streptomyces sp. Xuming Mao 46888 pMSP3545 PnisA Nisin Escherichia coli , gram-positive...Propionate Escherichia coli Jay Keasling 17810 pPro33 pPrpB Propionate Escherichia coli Jay Keasling 113634...

...pOpen-Endonuclease IV E. coli E. coli Endonuclease IV 165561 pOpen-Endonuclease VIII E. coli E. coli Endonuclease ...Endonuclease VIII 165571 pOpen-Exonuclease III E. coli E. coli Exonuclease III 165559 pOpen-T5gene12/13 T5 Exonuclease... pOpen-T4gene30 T4-DNA Ligase 165564 pOpen-ECOligA E. coli DNA Ligase 165569 pOpen-Tthlig Tth DNA Ligase...Open Enzymes parts for expression in Escherichia coli . Researchers can use the collection as the basis... vector of their choice and transforming into E. coli bacteria. This can help reduce lab costs and overcome...vector of their choice and then transform into E. coli for expression and purification ( pET28a-LIC or ... . Open Enzymes are also compatible with the E. coli Protein Expression Toolkit (Link opens in a new ...

...Methanocaldococcus janaschii are orthogonal in E. coli , while E. coli pairs are orthogonal in eukaryotic cells...tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG Jesse... E. coli photocaged cysteine Mammalian TAG Huiwang Ai 73544 pEvol-pAcFRS.2.t1 pAcFRS.2.t1 E. coli p-acetyl-l-phenylalanine...synthetase E. coli Mammalian TAG Simon Elsaesser 140019 pAS_4xEcoLeuT(CUA)_AnapRS AnapRS E. coli 3-(6-acetylnaphthalen...The amber codon is the rarest codon in Escherichia coli , which makes it the most commonly used choice. ...express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian cells. You should also... aminoacyl-tRNA synthetases (aaRS) for use in E. coli and mammalian cells. Many of these plasmids also...

...set : E. coli gene expression - Endy Lab CIDAR MoClo Parts Kit : Molecular cloning for E. coli - Densmore...plasmids is a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library...export laws and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide variety of... Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz Lab Bacterial Fluorescence Labeling...pCri System : Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP... purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK : Assembly of single and...CRISPathBrick Multiplex Plasmid : CRISPR expression in E. coli - Koffas Lab Additional Addgene Resources Synthetic...

...phosphorylated amino acid to the genetic code of E. coli (Park et al., 2011). Follow up work from the Rinehart...the parent protein. Using the recoded strain of E. coli , C321.ΔA (Bacterial strain #68306) , with the optimized...phosphosite library configuration for overexpression in E. coli . The phosphosite DNA library can also be introduced...flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation . Nature...Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment...2011). Expanding the Genetic Code of Escherichia coli with Phosphoserine . Science , 333 (6046), 1151–...production with rEcoli XpS and a new pSerOTS The Rinehart lab developed a new strain of recoded E.coli , rEcoli...

...Repeat) sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard...Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others) Drosophila...alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol...

...created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base...

...production. ID Strain Description PI 16398 BJ5183 E. coli strain for recombination between shuttle plasmid...

... Allows for in-frame fusions; NheI is rare in E. Coli genome. Prohibited Restriction Sites: EcoRI, SpeI...

...production of His6-MBP-tagged fusion proteins in E. coli SUMO Protein purification pCIOX - Bacterial expression...cleavage pExp-NusA - Expression of protein in E. coli as TEV cleavable N-terminal His and NusA fusions...

... various protein expression systems including E. coli , mammalian cells, and cell-free systems. NanoBiT...

...propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids are typically prepared using...

...Flp, are tyrosine recombinases derived from P1 E. coli bacteriophage and S. cerevisiae , respectively. ...

...Bioworks to distribute SARS-CoV-2 plasmids for E. coli , yeast, and mammalian expression, and spike ...

...and activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( ...

...luciferase gene allow for increased copy number in E. coli and expression of luciferase in the cytoplasm of...

...created by directed evolution of the Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine ...the DNA and replaces it with another base. In E. coli , this strategy yields C to A base edits, whereas...harnessed for antiviral mechanisms in bacteria ( E. coli ). Like the other Cas enzymes, the Cascade-Cas3 .... (2018). Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors....

... adapted an antibiotic resistance operon from E. coli : the tetracycline repressor protein (TetR) and ...

... repressing multiple target genes in Escherichia coli. Koffas Do you have suggestions for other plasmids...

... SED1 osmolarity biosensor, for expression in E. coli , yeast, or plants Intrinsically disordered protein...system. Front Cell Neurosci. 2013 Nov 13;7:202. Colin Akerman Chloride (Cl-) YFP-derived sensor for measuring...