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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 70 results for: EED

Showing: 1 - 20 of 70 results

  1. Plan Your Experiment

    Type
    Collection
    ...population need to be identical (clonal) or is a mixed population okay? Does the edit need to be homozygous...interference Needs good primer design Western blot Knockouts Activation/interference Needs a good antibody...other organisms. As with most experiments, you first need to have a biological question in mind. Then you ...will help you narrow down which components you’ll need for a given experiment. In the table below, we highlight...dCas9 to be fused to transcriptional activators that need to be within a given range of the transcription ...HEK293) cells. For more difficult cell types, you may need to look into other options. Browse Addgene's collection...cells are not transcribing DNA to mRNA, nor do they need to translate mRNA to protein. Instead, you directly...

  2. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid...type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9...45946 pU6-BbsI-chiRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles , Harrison , Wildonger...

  3. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...none, need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 ...Transfection none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...-scaffold SP6 AflII In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA...BioBrick-sgRNA T7 BbsI, BsaI Ingestion by C. elegans none, need Cas9 plasmid Ristow Do you have suggestions for ...

  4. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...expect to produce our guaranteed titer, and will give you the option to proceed with your order, or not...plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids with a cargo that may...not, without a guaranteed titer. Any titer expectations communicated by email will supersede the general...our viral production does not yield the titer guaranteed here or by email, Addgene will contact you to...that email within 10 business days in order to proceed with any adjustments to the original order. Turnaround...days of ordering. We will give you the option to proceed with your order, or not, with a longer turnaround...this email within 10 business days in order to proceed with any adjustments to the original order. Experimental...

  5. Antibody Guide

    Type
    Collection
    ...process may need to be optimized for individual assays. Clear cell debris by sedimentation. Proceed with IP..., an antimicrobial agent, can block some amines needed for conjugation reactions and should be removed...chosen. Signal Amplification The amount of signal needed from a specific antibody can vary depending on ...category, including an outline of the basic steps needed for each application, important controls, and any...samples to make proteins available. Tissue samples may need additional processing steps, such as sonication....signal levels. In ChIPseq, an input control will need to be collected before immunoprecipitation. Antibody...from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be...

  6. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target...’re using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9 BsaI E. coli...pgRNA-bacteria BBa_J23119 SpeI + HindIII Ampicillin none, need Cas9 plasmid Qi Do you have suggestions for other...

  7. CRISPR Plasmids - Yeast

    Type
    Collection
    ...type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target...’re using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...handle_RPR1t pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do you have suggestions for other...

  8. Resolute Plasmid Collection

    Type
    Collection
    ...human transmembrane transporters. Feedback Please send your feedback to the Resolute Partnership using... NeuroMab Collection Plasmids Pooled Libraries Feedback Solute carrier proteins (SLCs) are the largest...using their RESOLUTE repository feedback form . If you have further questions or comments you can also contact...

  9. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target...’re using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target...into the search bar. When using CRISPR, you will need to express both a Cas protein and a target-specific...

  10. CRISPR Plasmids - Plants

    Type
    Collection
    ...type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target...’re using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...

  11. CRISPR Guide

    Type
    Collection
    ...requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to the gene of ...Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting...) will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...NHEJ directly ligates the break ends without the need for a homologous template. Both NHEJ and HDR have... the depositing lab. After amplification, you’ll need to use next-generation sequencing (NGS) to verify...matches that of the original library. Next, you’ll need to generate lentivirus containing the entire library...

  12. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...transcription none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...

  13. AAV Molecular Tools

    Type
    Collection
    ...the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru...plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what you would...

  14. AAV Packaged on Request

    Type
    Collection
    ...affordable prices. While our repository serves the needs of many researchers, scientists sometimes require... our current in-stock capabilities. To meet that need, our Packaged on Request service lets scientists...Troubleshooting If your answer isn’t in the Help Center or you need more assistance with troubleshooting, we want to...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...modifications in endogenous loci with unprecedented speed and specificity. Figure 2: An overview of CRISPR...a DSB at the target genomic locus. Repair will proceed through NHEJ or HDR depending on the experimental... model system or biological topic, and you don’t need to be an expert to see results! Getting Started ...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    ...designed based on the target site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence...designed based on the target site sequence (30bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence... based on the target site sequence (21-22bp) and need to be followed on the 3' end by NGRRT or NGRRN PAM...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...transactivator may be needed. Or, when inducing a potentially toxic protein you may need extremely minimal...window) Resnitzky, D., Gossen, M., Bujard, H., & Reed, S. I. (1994). Acceleration of the G1/S phase transition...

  18. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid Chen Do you have suggestions for other...

  19. AAV Viral Preps

    Type
    Collection
    ...with each shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request service. ...search of viral preps and filter by the serotype needed....

  20. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...multiple orders would need to be placed through our website. Every order will need to be completed independently...
Showing: 1 - 20 of 70 results