... Special Collections Fluorescent Protein Guide Fluorescent Protein Plasmids & Resources You...Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When ...with a variety of fluorescent proteins, as well as over 100 empty backbones. Fluorescent Protein Kits cpFRET...this guide to learn about the applications of fluorescent proteins and to find the plasmids that are available...You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I Use? Blog: How to Choose...Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use this guide to learn more...more about the many applications of fluorescent proteins (FPs) and to find the plasmids that are available...

... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ... Green fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators...AMP) Yellow fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based ...SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing of Fluorescent Reporters for Imaging...Glucose Green fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based...

...Plasmid Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent Protein Resources... is not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been developed...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion...interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial...efficiency of the energy transfer is measured using fluorescence microscopy by exciting the donor and measuring...to as intramolecular or unimolecular FRET). Fluorescent proteins can be used as donor and/or acceptor...of plasmids suitable for creating individual fluorescently tagged proteins to study protein-protein interactions...

... Plasmid Collections Fluorescent Proteins Activity Regulation Fluorescent Proteins: Activity Regulation...Plasmids using a fluorescent protein as a scaffold to allow scientists to selectively modulate gene expression...Regulation More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics... Optogenetics Background Fluorescent proteins such as GFP have long been used for labeling, but new methods...methods are emerging to use these fluorescent proteins to modulate biological activity. Early tools allow...GFP-lines for cell-specific manipulation. Plasmids Fluorescent Protein Purpose Principal Investigator Plasmids...

... Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description Industry... Plasmid Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description Industry ...Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection More...Industry Greens Plasmid Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description...Davidson lab fluorescent protein fusion constructs and backbones. Plasmid... Plasmid Collections Fluorescent Proteins...More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab ...

...Imaging Guide Fluorescent Proteins: In Vivo Imaging More Fluorescent Protein Resources: Empty Backbones FRET...near-infrared, and photoactivatable fluorescent proteins. Plasmid... Plasmid Collections Fluorescent Proteins In Vivo Imaging...organs and whole mammals. Genetically encoded fluorescent probes are suitable for stable imaging of protein...undesirable background signals. Near-infrared fluorescent proteins for in vivo imaging are derived from...photoreceptors (BphPs). Spectrally distinct permanently fluorescent iRFP variants have been shown to have high effective...of the original iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein...

... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...Localization More Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid...Plasmid Collection FRET Biosensors Optogenetics Fluorescent Protein Blog Posts Background General Cytoskeletal...localization. One of the most common is to use fluorescence microscopy to compare the localization of your...markers. Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various ... should fuse your protein of interest with a fluorescent protein that has a different emission wavelength...

... Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones...which fluorescence intensity is 50% of its maximum value. Maturation is the time for fluorescence to obtain...collection of empty plasmid backbones with different fluorescent tags for you to create fusion proteins with your...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson...Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a ...a Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et...

... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...

...Photoconvertible , and Photoswitchable fluorescent proteins Various PIs Fluorescent protein fusion: contains empty...with N and C-terminal fluorescent protein tags that can be used to create fluorescent protein fusions with...cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein...yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein...pRSETa mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression: ...blot. The plasmids in this collection contain fluorescent protein tags whose localization you can monitor...Technique/Tag PI Purpose Empty Backbones with Fluorescent protein tags Various Plasmids Blue/UV , Cyan ...

... DULIP ( DU al L uminescence-based C o- I mmunoprecipitation) plasmids : Luminescence-based protein-protein...Double‐Readout Lu minescence‐based T wo‐ Hy brid) : Combines in vivo BRET with ex vivo luminescence-based co-immunoprecipitation...for assays of transcriptional regulation and bioluminescent reporters. Plasmid...Luciferase Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies...Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights...Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging...delivered intracellularly in order to measure luminescence. Gaussia luciferase is secreted and more stable...

...phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell...Reagents (ca. 2015) described in: A flexible codon in genomically recoded Escherichia coli permits programmable...Reagents (ca. 2014) described in: Enhanced phosphoserine insertion during Escherichia coli protein synthesis...Original Kit (ca. 2011) described in: Expanding the genetic code of Escherichia coli with phosphoserine...pSerOTS-C1* (V70) 188537 ). These reagents are described in detail in (Mohler et al., Mol Syst Biol 2023...second expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...Phosphorylated Isomers (iSPI). This new resource described in (Gassaway et al., Nature Methods 2022) is adapted...

...choice. ID Plasmid Description Promoter Cloning Method PI Return to top Fluorescent Proteins The table...below highlights plasmids that feature fluorescent or luminescent proteins for expression in plants. You...such as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter PI Return to top... You may also like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR...Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background Addgene’s...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...performs a chemiluminescent reaction in the presence of several different substrates. The luminescence produced...wells) by fluorescence activated cell sorting (FACS). Figure 7: Flow cytometry uses fluorescently-labeled...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent... to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...

...overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression...comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows...proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources...Goedhart J, Teusink B. In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep. 2019 Feb ... WA, Thorn KS. Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS...

...below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 214869... (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in... (Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny... (Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl... (Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong... (Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...BGHpA (Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium ...

...backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery of Custom Genetic...Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

...at the bottom of this page. For more in depth descriptions of each serotype, check out Addgene's blog on...Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato...Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP...Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...

... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights...plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells... Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism lineage ...to study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch ...Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic...zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab...

... virus. It also lists several luciferase and fluorescent reporter plasmids that have been used for measuring...RBD mutants that can be used to identify antibody escape mutants and for comprehensive mapping. Some of ... industry is continually growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids... SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations...plasmids related to these genes below. ID Plasmid Description Industry PI For more information on these genes...COVID-19 research in the table below: ID Plasmid Description Industry PI Do you have a suggestion for plasmids...Suggest a Plasmid form. If the plasmid has been described previously we will reach out to the lab to ask...