...Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...Calcium Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca(2+) indicator with...or astrocytes, (Constitutive or Cre-dependent) Li Lab GRAB_Ado Plasmids Yulong Li ADP A fluorescent, reagentless...detection (iAchSnFR) (Constitutive or Cre-dependent) A fast genetically encoded fluorescent sensor for faithful...Sensor (iGABASnFR) (Constitutive or Cre-dependent) A genetically encoded fluorescent sensor for in vivo imaging...

...99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression of cytoplasmic tdTomato...FLEx mGFP-2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP...Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent expression of diphtheria toxin receptor...45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for affinity...

...-ER2 AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome encoding Cre-dependent expression of the fluorescent protein...PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119741-AAV9 AAV SYN...PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119742-AAV5 AAV SYN...expression plasmid Scott Sternson 123308-AAV9 pAAV-hSyn-GRAB_NE1m Expresses the genetically-encoded fluorescent...in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...

... multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components... genome is encoded by plasmids called "transfer plasmids," which can be modified to encode a wide range... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table...used with 3rd generation transfer plasmids along with a plasmid encoding Rev Trono 12260 psPAX2 2nd Packaging...packaging plasmids that can be used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation...

... Plasmid Collections Optogenetics Plasmids Optogenetics Plasmid Collection You may ...Switch Plasmids Background Optogenetics plasmids encode proteins that are controlled by light. Many of ...Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch...of these plasmids encode for microbial opsins, which are membrane channels and pores derived from algae...Microbial Opsin Plasmids Browse, sort, or search the table below for the optogenetics plasmids for neuronal...ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter... top Optical Switch Plasmids Browse, sort, or search the table below for plasmids that use light to control...

...AAV-pgk-Cre PGK Cre expression Recombinases Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate...Cre.SV40 CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with... by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard...-EGFP Syn EGFP, Cre-dependent Control Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent...pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control...Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Harvey 137164...expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Deisseroth 55637 pAAV-EF1a-Flpo...

...transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...

... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...expressed. EXPRESs Plasmids for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright...Wright Lab. Collections of plasmids that encode zebrafish receptors or monoclonal antibodies to zebrafish...

...that encode site-specific recombinases, which can be used to control gene expression. See our Cre-lox ...Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name Promoter Fluorophore...Deisseroth 55636 pAAV-EF1a-Cre EF1a none rg* Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1,...Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following....CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry...105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Carmichael...

...expression. Overview of Viral Plasmids While plasmids are typically used to encode, propagate, and manipulate...been adapted into a plasmid-based technology, such that plasmids can be used to create viruses with specific...associated with better memory, plasmids can be used to create viruses that encode that protein. Then, those...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...genomes, such that they no longer encode for more virus, but instead encode for a specific gene or other research...

... Plasmid Collections EXPRESs Plasmids EXtracellular Plasmid RESource (EXPRESs) Consortium... Wright Lab plasmids EXPRESs is a collection of mammalian expression plasmids that encode the extracellular...commonly-used bait and prey plasmids can be found here: Plasmid Type Plasmid Name Bait Cd4d3+4-bio Prey...antibody plasmids P. falciparum merozoite surface protein plasmids P. vivax merozoite protein plasmids Human...Consortium plasmids that encode the extracellular regions of cell surface receptor and secreted proteins...approaches have created this resource to facilitate open and free exchange of these expression plasmids. It is ...to join our community and contribute their own plasmids in the future. AVEXIS: One popular method to detect...

...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites...

...dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent) Serotype...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral... in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information...Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D(...Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 44362 pAAV-hSyn-DIO-hM4D...Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Roth 50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-...

... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase...sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and...(backbone)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and...ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination and...sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination ...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested... vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2...

...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for ...zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (...different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can...

...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,...N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9...HSF1_Hygro MS2-P65-HSF1 Hygromycin lenti vector encoding the MS2-P65-HSF1 activator helper complex with... Browse our list of available viruses based on plasmids in our repository that express fluorescent proteins...

... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid...transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The ... Plasmid Gene/Insert Pooled Library The MORF pooled library is a comprehensive collection of human transcription...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...

...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat arrays...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...demethylase) made using these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine... TALE plasmids. Genome...

... Plasmid Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may...Chemogenetics vs. Optogenetics Chemogenetics plasmids encode genetically engineered receptors that are activated...Chemogenetics Plasmids Browse, sort, or search this table to find chemogenetics plasmids in our collection...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...