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  1. CRISPR Plasmids - CRISPR Transposases (CAST)

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated... CRISPR CASTs CRISPR Plasmids...Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick ...transposases (Tns) with the precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large fragments...below for CRISPR Transposase plasmids. Plasmids are available for expression in mammalian systems and bacteria...plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...classes of CASTs are type I and type V-K. Type I systems use a type I Cas effector, composed of several ...
  2. CRISPR History and Development for Genome Engineering

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    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas...endogenous Type II bacterial CRISPR system. Within the bacterial genome, a CRISPR array contains many unique...
  3. CRISPR Plasmids - Tagging

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists...PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids for CRISPR-based knock-in... CRISPR Protein Tagging CRISPR Plasmids... The CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...Tagging System The Eric Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based system...
  4. CRISPR Guide

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    ...inhibit Cas9 (CRISPR Type II) systems, while AcrV inhibits Cas12a (CRISPR Type V) systems. There are rare...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems contain two components: a guide...enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic edits...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...makes CRISPR an ideal genome engineering system for large-scale, forward genetic screening. CRISPR can ...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...
  5. CRISPR Plasmids and Resources

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    ...Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and the basics...planning your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial... are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout...RNA Targeting Type VI CRISPR systems target RNA, not DNA, across multiple systems. RNA Editing Cas13 enzymes...in mammalian systems. Parasites CRISPR plasmids designed for use in parasites. Plant CRISPR plasmids designed...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...
  6. Microbiology Resources

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    ...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check...software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The CRISPR technology was developed...synthetic biology. CRISPR Resources : Find essential background information on using CRISPR in your lab, specific... Check out our CRISPR reference page for CRISPR resources, such as depositor protocols and gRNA design...Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression...Expression Systems : Find plasmids to perform your experiment in a particular bacterial expression system. Fluorescent... specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology : Browse our deposited...
  7. Zebrafish Plasmid Collection

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    ...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...examples of useful zebrafish CRISPR resources can be found below: UFlip CRISPR targeted integration vectors...for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector...for the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...
  8. CRISPR Plasmids - Mammalian Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR...The following CRISPR plasmids have been designed for use in mammalian expression systems. Cut Fully functional...on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik lab, uses an MS2 hairpin-tagged...Marker PI Publication RNA Knockdown Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b...Selectable Marker PI Publication RNA Editing Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b...enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9...
  9. CRISPR Plasmids - RNA Editing

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type... CRISPR RNA Editing CRISPR Plasmids... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing...Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity...search the tables below for CRISPR plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...
  10. Plan Your Experiment

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    ...Select Expression System Design gRNA Publications Get Started CRISPR is a powerful system that enables researchers...including the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need both Cas9 and... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...you started using CRISPR in your research. Although we will use the example of CRISPR/Cas9 in mammalian...express the CRISPR machinery. For more difficult cells (e.g. primary cells), viral delivery of CRISPR reagents...summarizes the major expression systems and variables for using CRISPR in mammalian cells. Some of the...fluorophore) Delivery method Expression System Components of System Application Mammalian expression vector...
  11. CRISPR Plasmids - Cascade-Cas3

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...mechanisms. Cas3 belongs to the Class 1 family of CRISPR systems, the most abundant type found in bacteria and...below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand...
  12. CRISPR Plasmids - RNA Targeting

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In ...Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity...below for CRISPR RNA targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...be used to attentuate RNA levels. In mammalian systems, Cas13a does not exhibit the collateral RNA degradation...
  13. Zhang Lab CRISPR Page

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    ... the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system to facilitate... formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial...Cloning Protocol 237.2 KB SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing...
  14. CRISPR Plasmids - Purify Genomic Loci

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying... CRISPR Purify CRISPR: Purify Genomic ...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...essential to understanding locus function. Using CRISPR, researchers have expanded chromatin immunoprecipitation...(engineered DNA-binding molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify...co-expressing BirA biotin ligase, as seen in the CAPTURE system. The locus is subsequently isolated by affinity...
  15. CRISPR Plasmids - Base Edit

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...editing systems include targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...below for CRISPR base editing plasmids. Plasmids are available for expression in mammalian systems, bacteria...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR... CRISPR Base Edit CRISPR...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...
  16. Zhang Lab's CRISPR Frequently Asked Questions

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    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang...Zhang Lab's CRISPR Frequently Asked Questions You may also like... Zhang Lab CRISPR plasmids General CRISPR...using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn more. CRISPR Design FAQs Homologous...the Bench FAQs CRISPR Design FAQs Should I use wildtype or double nickase for my CRISPR genome engineering...donor. Alternatively, a new system described recently is the double nickase system, with PX335 vector, you...silent mutation. When attempting to use the CRISPR/Cas9 system to create specific mutations or insertions...such as PX459 ). After you co-transfect the CRISPR/Cas system with your homologous recombination (HR) template...
  17. Worm Expression Resources

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    ... individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's...genome editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified... tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use in C... feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and... for use together (e.g. cloning and expression systems) and therefore supplied as one item. Collections...
  18. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...CRISPR plasmids that create double strand breaks in DNA. CRISPR... Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower efficiency than NHEJ... . Browse, sort, or search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids ...
  19. Plant Plasmids and Resources

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    ... Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription...Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors,...species and genotypes - Zhenghe Li. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex...resources include tools for research using plant systems such as Arabidopsis, wheat, maize, rice, tomato...technology or the Plant CRISPR Resources for a full selection of plasmids expressing different functional variants... gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose Toolkit enables...
  20. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

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    ...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols...information about RGNs and CRISPR-Cas systems can also be found at http://www.crispr-cas.org . Users are also... Genome Engineering CRISPR Joung Lab CRISPR Plasmids...Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II CRISPR-Cas ...variant) Engineered CRISPR-Cas9 nucleases with altered PAM specificities Dimeric CRISPR RNA-guided FokI Nucleases... Nucleases (RFNs) CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression ...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...
Showing: 1 - 20 of 57 results