We narrowed to 41 results for: invs

...of the DNA rearrangement after recombination (inversion, deletion, or translocation) depends on the orientation...opposite directions, recombination results in an inversion and the region of DNA between the target sites... loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13 bp inverted repeats + 8 bp spacer (34 bp) lox2272, lox5171...Dre rox TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted repeats + 4 bp spacer (32 bp) Currently, only ...GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC 13 bp inverted repeats + 8 bp spacer (48 bp or 34 bp) F3, F5,...arranged, recombinases can be used for more than inversion, deletion, or translocation of a gene of interest...flip-excision”): Also known as DIO (Double-floxed Inverse Open reading frame), this system allows scientists...

...infection, such as ACE2 and TMPRSS2, which are involved in the entry of SARS-CoV and SARS-CoV-2. Find ...protease that primes the SARS-CoV-2 S protein and is involved in virus entry into cells (Hoffman et al., 2020...superfamily, binds to the SARS-CoV-2 S protein and is involved in virus entry into cells (Wang et al., 2020)..... PCP4 plasmids - (Purkinje cell protein 4) is involved in beating of airway cilia (Kogiso et al., 2020...

...Multiplex Genome Engineering Many CRISPR experiments involve editing more than one gene. In these cases, delivering...using Cas9 to generate large genomic deletions or inversions with two gRNA sites on the same chromosome. Most...phases. Chemically or genetically inhibiting genes involved in NHEJ or upregulating those in the HDR pathway...flexible than base editing: it can encode edits involving any new sequence (point mutations, insertions,...for conducting genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: ...Modifications Using CRISPR A variation of CRISPRi/a involves dCas9 fused to epigenetic modifiers. These programmable...off-target alterations, such as small mutations or inversions, than do large deletions generated by Cas9 systems...

...as a powerful defensive strategy against viral invaders, and this system is proving to be just as useful...CRISPR-associated (Cas) genes to not only store a record of invading phages but also to destroy the phages upon re-... the foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview of the endogenous...to ensure prokaryotic survival, but it is not invincible. Bondy-Denomy et al. identified the first set...

...CRISPR plasmids to study the regulatory networks involved in retinal development....Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...aide in their studies of the regulatory networks involved in retinal development the lab utilizes a CRISPR...

...212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription Debrya Groskreutz...hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription Pete Stecha...212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription Pete Stecha...

...) is a microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci...increase targeting efficiency. These plasmids use the inverted terminal repeats (ITR) from AAV serotype 2. SaCas9...

... experimental design, biosensors can enable investigation of a signaling pathway or measurement of a biomolecule...Calcium Red calcium sensor FRCaMPi and SomaFRCaMPi, inverted topology of FRCaMP (Fungus based Red CalModulin...2011;6(12):e28245. Uwe Sauer Citrate Direct or inverse fluorescent biosensor for citrate (Citron/Citroff...cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors for Citrate...

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...

...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...

...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list ...100 µL sized sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample...

...receive a ready-to-use viral preparation from the inventory below. Lentiviruses are distributed as purified...Use pooled CRISPR libraries to screen for genes involved in specific biological processes. For more information...

...variety of pathways, which ultimately turn on genes involved in cell growth, differentiation and survival. ...Transcription factor Dp TIAM TIAM1 TIAM2 T-cell lymphoma invasion and metastasis TP53 Tumor protein p53 TSC TSC1...

...symptoms through medication and surgery. PD primarily involves the malfunction and death of dopamine-producing...other large scale studies. Researchers are now investigating the role that these additional genes may play...

...Basic Vector - Firefly luciferase vector for investigating regions controlling transcription See our Luciferase...BiFC vector (pEarleyGate201-YN) Zeocin® is an InvivoGen trademark. Return to top Content last reviewed...

...producing high amounts of protein. Whether you're investigating basic bacterial gene biology or engineering ...containing recombinases to induce targeted genetic inversion, deletions, and translocations events. Visit our...

...immunology collection contains plasmids for genes involved in innate and adaptive immune signaling and cell-cell...such as macrophages and neutrophils that attack invading pathogens. Innate immune cells also process and...membrane and can also function as signaling proteins involved in proliferation, cell differentiation, and apoptosis...molecule, major histocompatibility complex, class II invariant chain DHLAG, HLADG, Ia-GAMMA CD8A CD8a molecule...

...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...

...valued opportunities for further translational investment. All resources and high-quality research tools...

...signaling pathway, focusing on the main pathways involved in cancer development. These pathways are important...