...with transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83...somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. ... temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek Fibroblasts...conversion of human fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8...self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32....

... Site-Specific Recombination in Transgenic Mice. Proc Natl Acad Sci. Jul 15;89(14):6232-6. PubMed . Matsuda...Transgenes Introduced by In Vivo Electroporation. Proc Natl Acad Sci. Jan 16;104(3):1027-32. doi: 10.1073...Cells by the Cre Recombinase of Bacteriophage P1. Proc Natl Acad Sci. Jul;85(14):5166-70. PubMed . Sternberg...Cre-lox-Regulated Conditional RNA Interference from Transgenes. Proc Natl Acad Sci. Jul 13;101(28):10380-5. doi:10.1073...Mammalian Capecchi 126006 pAAV2-ProC3-Cre/mCherry Cre and mCherry ProC3 AAV Roska 126700 pHAGE2-EF1aL-Cre-IRES-NeoR-W...floxed portion of a construct during the cloning process). VCre and SCre are alternative Cre recombinases...the stop codon is excised, and gene expression proceeds. Cre-dependent Gene Knockout: Conversely, putting...

...improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9...and Golgi Zn2+ with genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. ... cells revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann...high-frequency release at Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115...in muscles and neurons of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2019 Aug 1. Alexander Gottschalk..., the activity of specific genes and cellular processes, or other factors like environmental conditions...largely preserving spatial and temporal cellular processes. Note that many of the articles linked below include...

...understand its function and pathology. Mohanty et al. Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral...disease and implicate a wide range of cellular processes. The vast majority of cases (90-95%) of ALS are...al. Cell Rep Methods. 2022 Mar 28. Follow APP processing and dynamics using APP and Bace1 plasmids . Capitini...

...MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94...

...CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A . 112(10):3002-7. PMID: 25713381...arrays. Separate Cas proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs...-CRISPR RNA (pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating...non-homologous end joining (NHEJ) , an error-prone process that produces insertions/deletions disrupting a...a DSB at the target genomic locus. Repair will proceed through NHEJ or HDR depending on the experimental...

...randomized reorganization process, creating a unique binding site. This process, known as V(D)J recombination...delaying a process through sequential rounds of validation. Validation of an antibody is a process separate...binding partners, location in the cell, and sample processing can hide or reveal epitopes on an antigen. Monoclonal...can interfere with the conjugate or conjugation process. Sodium azide, an antimicrobial agent, can block...proteins to detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody...available. Tissue samples may need additional processing steps, such as sonication. Denature proteins,...proteins from a complex mix. Overview of application Process the tissue or cell culture samples to extract proteins...

...guaranteed titer, and will give you the option to proceed with your order, or not, without a guaranteed titer...that email within 10 business days in order to proceed with any adjustments to the original order. Turnaround... by your institution. Our final step of the QC process, viral genome sequence verification, may not yet...days of ordering. We will give you the option to proceed with your order, or not, with a longer turnaround...this email within 10 business days in order to proceed with any adjustments to the original order. Experimental...thorough shipping documents and following import procedure, it is the responsibility of the importer to pay...

...purification Addgene’s comprehensive quality control process: Titering Purity assessment Sterility testing Viral...plasmid on its material page. Request and Ordering Process AAV production will take approximately 6–8 weeks...information must be added before we can begin processing your order. MTA Approval 1+ business days, varies...from the providing scientist through an MTA. This process typically takes 1–4 days, depending on your institution...prep, and perform comprehensive quality control processes to make sure it is ready for your experiments....

...facilitate the processing of the crRNA) is also expressed from a separate promoter. In the pre-processing form,...construct from our 'Split RNA' design, where the pre-processing form of crRNA is expressed and a tracrRNA (the...in cell and with Cas9 and tracrRNA, it will be processed (i.e., cleaved) into a shorter form that contains...our 'Chimeric RNA' design (meaning the mature processed form of crRNA is fused chimerically to a mature...

...more about Addgene’s MTA process please visit our Help Center . The MTA Process: Once your order has been...approve the MTA documents, your order will be processed and shipped. Industry Distribution Policy A portion...

...fuel these energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome...regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein and lipid synthesis...

...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and...Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression ...Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple functional gRNAs...

...15574 GAL 25Q+ProCFPp303 HTT CFP GAL1 Huntington's Susan Lindquist 15575 GAL 103Q+ProCFPp303 HTT CFP GAL1...15578 GAL HA25QDProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist 15579 GAL HA103QDProCFPp303 HTT HA... Huntington's Susan Lindquist 15570 GAL HA25Q+ProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist...GAL1 Huntington's Susan Lindquist 15584 GAL FLAG25QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist...Lindquist 15585 GAL FLAG46QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist 15586 GAL FLAG72QDProCFPp303...GAL1 Huntington's Susan Lindquist 15589 MET FLAG46QDProCFPp303 HTT FLAG, CFP MET1 Huntington's Susan Lindquist...Lindquist 15590 MET FLAG72QDProCFPp303 HTT FLAG, CFP MET1 Huntington's Susan Lindquist 15591 MET FLAG103QDProCFPp303...

... our production process? At Addgene, we provide transparency in our production process by...

...Addgene collection? Click here to start the deposit process and have your plasmids added to the list. You can...more information and assistance with the deposit process. Want more information on the wide variety of Cas...

...mechanism. Since it is pre-processed, it can bypass the Dicer cleavage step and proceed to binding of RISC. ...

...also kindly provided this protocol detailing the process for packaging these transfer vectors and generating...pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-white screening and forms...

...is necessary to model your specific disease or process of interest. Do you want to: Generate complete ... method of delivery and the cell type. Before proceeding with your experiment, it may be necessary to ...

... present, the introns are excised during mRNA processing, producing a functional molecular tool (C,F)....Deisseroth Lab maintains a Standard Operating Procedure (Link opens in a new window) with general principles...