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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 137 results for: ica

Showing: 11 - 20 of 137 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...differentiate into multiple cell types, but are typically limited to related cell lineages. Pluripotent ...organism. iPSC technology removes the possible ethical concerns related to embryonic stem cell use and...generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming...Blood Cells. Stem Cells. 2012 Nov 29. Yamanaka Replicating EBNA1 episome Human Non-integrating EBNA1-mediated... Nat Methods. 2011 May;8(5):409-12. Yamanaka Replicating EBNA1 episome Human Non-integrating polycistronic... S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-...Stem Cell Res Ther. 2017 Jun 5;8(1):132. Zovein Replicating EBNA1 episome Human Non-integrating EBNA1-mediated...

  2. Bacterial Expression Systems

    Type
    Collection
    ...for protein purification. The pTD Plasmid Series contains plasmids suitable for replication in many gram-negative...expression plasmids that can be used for protein purification, protein visualization, study of protein interactions...Systems Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled...Bacteria are commonly used to create, store, and replicate plasmids of all types, but beyond that, researchers...bacteria as model systems to answer many interesting biological questions. Escherichia coli is the most widely...expression plasmids to find out more! Protein Purification To study the function, structure, and activity...through affinity chromatography. To facilitate the purification process, proteins are often expressed alongside...

  3. Rett Syndrome

    Type
    Collection
    ... opens in a new window) ClinicalTrials.gov - a list of Rett syndrome clinical trials (Link opens in a ...window) PMID: 16615965 Leonard et al. 2017. Clinical and biological progress over 50 years in Rett syndrome...months of age. A defining feature, with onset typically between 18 to 30 months, is regression of previously...of Rett syndrome is currently based on specific clinical diagnostic criteria, defined as the presence of...MECP2 gene. Causative mutations for Rett syndrome typically arise spontaneously, although in rare cases Rett...determines a minimally functional MECP2 that rescues neurological defects of Rett syndrome. (Link opens in a new...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... the original publication below. You can also download the full-text of the publication here . Article...given the efficiency of biallelic modification, ease of rapid identification by PCR, predictability of loss-of-function...therapy and research. Gehl J. Acta Physiologica Scandinavica . 2003. PubMed . Purifying plasmid DNA... of Visualized Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs details...addition, we describe techniques for deletion, identification, and characterization. This strategy relies...to help identify guide sequences that minimize identical genomic matches or near-matches to reduce risk...band” is often too large to efficiently amplify. Typically use primers at least 100 bp from the predicted...

  5. Institute for Protein Innovation

    Type
    Collection
    ... includes anti-glypicans that accurately detect glypican presence and explore glypican function, providing...about IPI (Link opens in a new window) Glypican Antibodies Glypicans are a family of highly conserved proteoglycans...Institute for Protein Innovation Antibody Collection Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies...families of cell surface and secreted proteins (glypicans and integrins) and common epitope tags, as well...scientists worldwide. Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description...Description Isotype Reactivity Recommended Applications Integrin Antibodies Integrins are a family of mechanotransduction...Antibody Description Isotype Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed...

  6. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Insert Promoter Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase...Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional...Insert Promoter Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

  7. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Parasites...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

  8. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...the most abundant forms of posttranslational modification in cells and research into its many roles in...Yale have made improvements to this system by genomically recoding E.coli (Lajoie et al., 2013) and improving...popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus plasmid...subpools of ~11,000 unique phosphosites. Iterative Synthetically Phosphorylated Isomers (iSPI) The iSPI resource...pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated Isomers (iSPI) described in: ...an orthogonal translation system with enhanced biological tolerance . Molecular Systems Biology , 19 (8...phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions ....

  9. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...make targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic ...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...enhancer for your gene of interest. Available modifications include: histone acetylation by p300 histone...or MQ1 cytosine demethylation by Tet1 These modifications persist over time and are potentially heritable...tables below for CRISPR plasmids for epigenetic modification in mammalian or plant systems. Mammalian ID ...

  10. CRISPR Plasmids - Plants

    Type
    Collection
    ...Gene/Insert Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase...Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...
Showing: 11 - 20 of 137 results