We narrowed to 116 results for: ANT

...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids... topology, size, and isoelectric points. OpenPlant Kit Plant Expression, Genome Editing Jim Haseloff 79...and vectors for genome engineering in the plant model Marchantia polymorpha . Pooled Libraries Pooled Library... our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications PI Don't...Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering and MTA Information Featured Collections...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...junction sets for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for...

... use to scientists. Antibodies ID Recombinant Antibody Description PI 211756 Anti-SARS-CoV-2 Nucleocapsid...Nucleocapsid Protein [mBG17] Clone mBG17, recombinant mouse monoclonal antibody targeting the SARS-CoV-2 nucleocapsid...Brian Geiss 211757 Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] Clone mBG86, recombinant mouse monoclonal...COVID-19 and Coronavirus Plasmids & Resources Antibodies SARS-CoV-2 Plasmids Other Coronavirus Plasmids...monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein. Brian Geiss Plasmids SARS-CoV-2 Plasmids Browse...Scientists Map the SARS-CoV-2-Human Interaction Network Antibody Neutralization Response Against Pseudoviruses ...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Return to top Other Coronavirus Plasmids: SARS-CoV, MERS...

... fusion. See 36083 and 36086 for other FP variants. Pantelis Tsoulfas 41393 pCW57.1 3rd Inducible lentiviral...both dividing and non-dividing cells without a significant immune response. These viruses also integrate...expression. See Ventura et al. (2004) for more variants. Tyler Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP...transgene or shRNA. See Szulc et al. (2006) for more variants. Patrick Aebischer and Didier Trono 11795 pLL3.7...markers. See Campeau et al. (2009) for additional variants. Eric Campeau 39481 pLenti-puro 3rd CMV-driven...selection. See Shin et al. (2006) for additional variants. Iain Fraser 15948 pLOVE 3rd Tet inducible gateway...destination plasmid for cDNA expression. Miguel Ramalho-Santos 24129 pULTRA 3rd Bicistronic expression of EGFP...

...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids...partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...monoclonal antibodies validated for neuroscience research. The plasmids encoding these antibodies are available...Initiative collection of plasmids, viral preps, and antibodies here. Collections...community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN Initiative...identified by depositors and/or when BRAIN Initiative grants are noted in publications associated with Addgene...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...

...(knockout)? Generate a specific mutant allele of a gene (point mutant)? Increase or decrease expression...of a selectable marker (antibiotic resistance or fluorophore) are also relevant. Delivery Using Plasmids... specific disease or process of interest. Do you want to: Generate complete and permanent loss of gene... window from the PAM sequence, and base editor variants often have different optimal editing windows. ...testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference : Target...Predicted On-Target and Off-Target Activity Another important factor to keep in mind when designing your gRNA...In addition to off-target activity, it is also important to consider factors that maximize cleavage of ...

...PubMed PMID: 24379683 . When mutants gain new powers: news from the mutant p53 field. Brosh R, Rotter V...to activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization... of p53’s pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional...-of-function, expanding the mechanisms by which mutant p53 is known to function in cancer. p53 Pathway...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...2003 Mar;21(3):313-20. PubMed PMID: 12619118 . p53 mutant mice that display early ageing-associated phenotypes...of p300/CBP in p53-dependent signal pathways. Avantaggiati ML, Ogryzko V, Gardner K, Giordano A, Levine...

...apoptotic ablation of neurons through a process reliant on Flp recombinase. 5 Kazunari Miyamichi Genome...-mPCSK9 hAAT-driven, constitutive Expression of mutant (D377Y) murine PCSK9 for studying hypercholesterolemia...-hPCSK9 hAAT-driven, constitutive Expression of mutant (D374Y) murine PCSK9 for studying hypercholesterolemia...hSyn-driven, constitutive Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with...membrane-tethered ibARK in astrocytes. 5 Jun Nagai Anterograde Transsynaptic Tracers These AAV encode tools ...that can be used to map neural circuits in the anterograde direction across synapses. Also see Monosynaptic...232351 pAAV-ATLASsnCre Syn-driven, constitutive Anterograde transsynaptic tracer protein to express in presynaptic...

...first set of anti-CRISPR genes in bacteriophages infecting Pseudomonas aeruginosa . Anti-CRISPR genes ...Cpf1) Type IV (Csf1) Type VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive immune...complex. Recently, researchers have begun to use anti-CRISPRs as off-switches for CRISPR genomic editing...libraries can be used to identify genes that are important to a given phenotype. Current libraries are available...editing rates. Advances in this area include: Nickase mutant: cuts only one strand of DNA and can be used with... High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very...to be used together. Researchers have engineered mutant Cas enzymes with alternative PAM sites . Cpf1 ,...

...: SepRS adds a phosphoserine to tRNA^Sep with anticodon, CUA. Engineered EF-Tu binds the phosphoserine-charged...Phosphorylation Protein phosphorylation is one of the most abundant forms of posttranslational modification in cells...University changed the way researchers can explore important questions surrounding serine phosphorylation by...overall improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli... of the popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus...phosphosite library (Pooled Library #111704) is anticipated to be useful for laboratories performing phosphoproteomic...reference spectra or phosphopeptide standards for quantitative assessments. The Mode #1 phosphosite library...

...Tissue Bank Antibodies (Link opens in a new window) N-terminal Anti-MECP2 (M7443) mouse antibody from Sigma...C-terminal Anti-MECP2 (M6818) mouse antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2 ... expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these X inactivation ratios ...syndrome at the molecular level. They are also an important tool for developing therapeutic strategies to ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

... Medicines Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives... production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid..., Christodoulaki, E., Teoh, S. T., Leippe, P., Santini, B. L., Rebsamen, M., Lindinger, S., Serrano, I... I., Onstein, S., Klimek, C., Barbosa, B., Pantielieieva, A., Dvorak, V., Hannich, T. J., Schoenbett, ...Hepworth, D., Liu, S., Noell, S., Piotrowski, M., Stanton, R., Zhang, H., Corallino, S., Faedo, A., Insidioso...Pütter, V., Zindel, D., Hamilton, B., Lenter, M., Santacruz, D., Viollet, C., Whitehurst, C., Johnsson, K....

... protein component of MMR) mutant to inhibit MMR PE5 — PE3 plus MLH1 mutant to inhibit MMR PEmax — optimized...QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao 141081 pYPQ141D-peg Plant OsU3 pegRNA ...Plasmids: Prime Edit Mammalian Bacteria Drosophila Plant Empty pegRNA Resources Prime editing is a “search... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...BbsI + PstI for pegRNAs No Xiao Wang 170132 pOsU3 Plant OsU3 pegRNA BsaI + HindIII No Caixia Gao 172716 ...

...High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3)...Mutated Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient Transduction of Trabecular...Petrs-Silva, et al. 2011. Novel properties of tyrosine-mutant AAV2 vectors in the mouse retina. Mol Ther . Feb...mesenchymal stem cells into pluripotent stem cells by a mutant adeno-associated viral vector. Hum Gene Ther Methods...in Cynomolgus Macaques of rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin...Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol . Mar... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...maximum excitation wavelength (nm), QY D : Donor quantum yield, λ Aem : Acceptor maximum emission wavelength...exctinction coefficient (M -1 cm -1 ), QY A : Acceptor quantum yield, R 0 : Förster radius (nm, distance at which...linkers of varying length Evers et al. (2006) Quantitative understanding of the energy transfer between...FRET efficiency standards Thaler et al. (2005) Quantitative multiphoton spectral imaging and its use for...stoichiometry of protein interactions using image-based quantitative two-hybrid FRET Positive and negative controls...evolution of cyan fluorescent proteins towards a quantum yield of 93% Positive and negative controls and...FRET pairs Gadella et al. (2023) mScarlet3: a brilliant and fast-maturing red fluorescent protein Variety...

...Mammalian Bacteria Drosophila Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand...Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair...created by wild-type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types... for use in cancer signaling studies, including mutant, knockdown, and overexpression constructs. Each...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...location, their pathway dependencies are extremely important for classification and treatment. On each pathway...Meyerson , and Root Labs containing wild-type and mutant alleles described in the initial Target Accelerator...

...through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL for the AAV1... you know that we don’t expect to produce our guaranteed titer, and will give you the option to proceed...proceed with your order, or not, without a guaranteed titer. Any titer expectations communicated by email ...email will supersede the general guarantees above. We will have our expert viral vector production team carefully...our viral production does not yield the titer guaranteed here or by email, Addgene will contact you to...carried out with our materials, Addgene cannot guarantee the function of any viral vector. Addgene performs...

...European Vector Architecture Open Plant (Link opens in a new window) — Plant SynBio resource Synthetic Biology...Mukhopadhyay Richard Murray Erin O'Shea Martin Parniske Antonio Richart Herbert Sauro Claudia Schmidt-Dannert Pamela... Organism Bacterial Yeast Fungal Worm Mammalian Plant Algal Featured SynBio Deposits Alper Lab Y. Lipolytica...FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit ...Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...AAV together can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2...the cell population of interest with a deletion-mutant tracing virus that lacks the ability to infect ... To enable monosynaptic spread of this deletion-mutant rabies, the essential G protein is provided to ...2007a. Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

... to enhance protein solubility and to direct recombinant proteins to the periplasmic space between the...bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium Modular Assembly Kits...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...leads to slow bacterial growth, or maybe you only want to study the effects of protein expression in the...the stationary phase. In these cases, you may want to control protein levels or to turn on expression ...presence of that small molecule and sometimes even quantify it. Bacterial one-hybrid (B1H) systems also use... or introducing specific mutations can reveal important insights into gene function. These genetic changes...