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We narrowed to 74 results for: ATO

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Showing: 21 - 40 of 74 results

  1. Fluorescent Proteins: FRET

    Type
    Collection
    ..._C1 mT-Sapphire* tdTomato 399 0.6 581 138,000 0.69 6.4 5.9 mT-Sapphire-N1 , tdTomato-N1 CyOFP1* mCardinal...mCardinal-N1 mAmetrine* tdTomato 406 0.58 581 138,000 0.69 6.5 7.1 mAmetrine-N1 , tdTomato-N1 mTurquoise2 sREACh... nm 4 ). Values obtained from FPbase.org FRET calculator (Link opens in a new window) . *: Large Stokes...pairs Michael Davidson Lab (Unpublished) FRET Indicators - Live and Fixed Cells Additional Resources at...new window) External Resources FPbase.org FRET calculator (Link opens in a new window) Nikon MicroscopyU...

  2. CRISPR References and Information

    Type
    Collection
    ...Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode: Injection... dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression... Lab and maintained by the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder...pAC91 , pAC92 , pAC93 , pAC94 , pAC95 ; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 , pAC148...; Gateway destination: pAC90 PDF, 1.1 MB Katic Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone...

  3. Biosensor AAV Preps

    Type
    Collection
    ...Rose 51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f dTomato Cre dependent rg* Jonathan Ting 51085...51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f dTomato Constitutive 1, rg* Jonathan Ting 52924 pZac2.1...Rose 51082 AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato EF1a GCaMP6s dTomato Cre dependent 1 Jonathan Ting 105714...Larsen 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s dTomato Constitutive 1, rg* Jonathan Ting 68717...

  4. mTOR Pathway

    Type
    Collection
    .... enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many cancers...arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation... caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer...Ras-related GTP binding Raptor Also known as RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras...Catalytic Subunits PIK3CA PIK3CB PIK3CD PIK3CG Regulatory Subunits PIK3R1 PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6...Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC PKC alpha PKC beta PKC...

  5. Worm Expression Resources

    Type
    Collection
    ...Constructs Kits and Collections Other Resources The nematode Caenorhabditis elegans ( C. elegans or “the worm... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...contains 29 plasmids used to generate transgenic nematodes, including C. elegans and C. briggsae. Other related...A database featuring behavioral and structural anatomoy of C. elegans. WormBase - A consortium of biologists...genomics and biology of C. elegans and related nematodes. WormBook - A comprehensive, open-access collection...related to the biology of C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the...

  6. Lentiviral Prep Service

    Type
    Collection
    ...dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic...dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti dCAS-VP64_Blast ...Celltag library contains 19973 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 4934 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 5737 barcodes to combinatorially index cells for single-cell analysis of clonal...

  7. TALEN Engineering

    Type
    Collection
    ...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and..., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013...dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the construction ...interest for potential TALEN or TALE transcriptional activator target sites and then generates individual user-friendly...REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast and FLASH...

  8. Deisseroth INTRSECT Collection

    Type
    Collection
    ...fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting...INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular targeting...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...Flp AND NOT Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...window) Cummings KA, Clem RL. 2020. Prefrontal somatostatin interneurons encode fear memory. Nat Neurosci...

  9. Synthetic Biology - Overview

    Type
    Collection
    ...and synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also... Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches...

  10. p53 Pathway

    Type
    Collection
    ..., which disrupt its interaction with negative regulators, increase its stability and DNA binding activity...activity, and allow it to bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional...proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-...peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53 apoptosis...binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... pFA6a-link-tdTomato-SpHis5 - Yeast Expression tdTomato-N1 - Mammalian Expression tdTomato-C1 - Mammalian...- Bacterial Expression tdTomato 554 581 95 4.7 1 h Tandem-dimer pCSCMV:tdTomato - Mammalian Expression...Mammalian Expression tdTomato-pBAD - Bacterial Expression Return to top Red Protein Excitation (nm) Emission...

  12. NETRF

    Type
    Collection
    ...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...

  13. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Figure 1: Overview of ...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with...screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein complex for the...: lentiviral vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker...JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):...

  15. Plant Plasmids and Resources

    Type
    Collection
    ...systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to...Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR parts...using TALENs and CRISPR. Successfully validated in tomato, tobacco, Medicago truncatula , wheat, and barley...

  16. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Examples Include: promoters and terminators repressors and activators logic gates Networks and Gene Regulation...includes both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and...

  17. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...support educators interested in integrating MDH-related research into their teaching laboratories. They ...professional development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor...

  18. Recombinases AAV Preps

    Type
    Collection
    ...mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, 2, 5, 8, 9, rg*, PHPeB Rylan Larsen 107787... none 1 Connie Cepko 69916 pAAV.cTNT.iCre cTnT tdTomato 9 William Pu 203842 pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Harrison 37351 pQC membrane TdTomato IX Membranes Palmitoylation tdTomato Connie Cepko 22479 FUmGW Membranes...118737 pBOB-CARMIL2 BH domain-tdTomato Plasma membrane CARMIL2 BH domain tdTomato John Cooper 189771 pLCK-mScarlet3...

  20. Arf GTPase Family

    Type
    Collection
    ...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs....References The ARF (ADP-ribosylation factor) family of regulatory GTPases have long been characterized as members..., 2016). Though best known for their roles as regulators of membrane traffic, they also play critical ...
Showing: 21 - 40 of 74 results