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... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...Overview of Cas9n. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert... 5: Overview of base editing. More recently, researchers have developed base editors that can catalyze...Base Edit CRISPR Prime Editing Prime editing is a “search and replace” gene editing method in which Moloney...

...system of choice, promoter, or search by inducible system (e.g., search for "split", "rapamycin", “tamoxifen...been limited by low integration efficiency but research groups continue to optimize these tools. The best...recombinase (Cre, Flp, Dre, other recombinases). Use the search bars or sort buttons to find plasmids based on ...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Flp recombinase...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Dre recombinase... gene expression may be activated or repressed. Search by recombinase (Cre, Flp, Dre, etc.) or target ... sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-, Flp-, or Dre-dependent...

...Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to find plasmids... Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies...Protein Labeling from New England Biolabs Cancer Research from Sinai Health System Plasmids from the Structural... Library Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation ...open, efficient and affordable access to plasmid research tools. Pricing will be indicated on the product...

...In order to produce recombinant AAV vectors, researchers need to use three plasmids: A transfer plasmid...transfer plasmids in Addgene's repository. You can search or sort this table based on plasmid name, gene ... expressing Rep2 and Cap2 Melina Fan , Addgene Research Program 232922 pAAV2/5 AAV5 AAV packaging plasmid... expressing Rep2 and Cap5 Melina Fan , Addgene Research Program 240485 pAAV2/6 v1 AAV6 AAV packaging plasmid...expressing Rep2 and Cap6 Meghan Rego , Addgene Research Program 112863 pAAV2/7 AAV7 AAV packaging plasmid...expressing Rep2 and Cap11 Meghan Rego , Addgene Research Program 112866 pAAV2/rh10 AAVrh10 AAV packaging...separate helper plasmid during AAV production. You can search or sort this table based on plasmid name, capsid...

...possible function of a protein of interest. Browse or search the table below to find markers of general cellular...Mykytyn *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...necessary for movement and cell division. Browse or search the table below to find markers of cytoskeletal...Harrison *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...junctions, tight junctions, and desmosomes. Browse or search the table below to find markers for cellular junctions...Fanning *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein... autophagosomes, lysosomes, and more. Browse or search the table below to find markers for components ...

...synthetic biology collections) for use in C. elegan research....been developed and characterized which allow researchers to express desired constructs in precise cell...highlights plasmids that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...Andrew Fire Lab. A set of vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans...computer scientists dedicated to providing the research community with accurate, current, accessible information...

...While our repository serves the needs of many researchers, scientists sometimes require preps beyond our...Packaged on Request service is recommended for research-grade use. Many chemogenetic, optogenetic, recombinase... methods to troubleshooting insights, you make research more reproducible for scientists everywhere and...opens in a new window) for producing high-quality research-grade viral vector preps. Data Hub Access Addgene...vectors. Our community-driven Data Hub connects researchers with troubleshooting and results that complement...Help Center Check out Addgene’s Help Center, a searchable database of information to help you every step...

...Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Color is used for clarity...Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Return to top Ras Pathway... Cancer Research Ras Pathway Ras Pathway You May Also Like: Ras...gene expression vectors for in vitro and in vivo research. The RAS Initiative - at the National Cancer Institute...

...Plasmid Collection (Link opens in a new window) Researchers developing novel diagnostics, therapeutics, and...important experiments. In an effort to speed up research of SARS-CoV-2 in early 2020, Ginkgo Bioworks (...synthesis capabilities, in collaboration with researchers at Stanford University’s Department of Bioengineering...Bioengineering, to build DNA that would enable researchers to express SARS-CoV-2 viral proteins in several...validated by Ginkgo or Addgene. Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common...

...Disease Research Find deposited plasmids for specific disease research areas. Cancer Research Plasmids...Collection Neuroendocrine Tumor Research Foundation Neurodegeneration Research Collection Rett Syndrome Plasmid...

...intruders, antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly to a...name a few. Traditional sources of antibodies for research purposes include animals or hybridoma cells, but...antibodies extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant...expression of antibodies, nanobodies or ScFvs. Use the search bar or sort buttons to find antibody plasmids for...Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description...

... to academic, nonprofit, and industry researchers under research use only (RUO) terms and do not require...Link opens in a new window) Explore cutting-edge research tools from Promega — now available through Addgene...to find plasmids in the collection. For example, search by response element, reporter, protein, or tag,..."NanoLuc", "HaloTag", "BiT", or other terms. Or search the collection (Link opens in a new window) on ...

...with fairly high specificity. Fifteen years of research has shown that when fused to a nuclease or activation...interview with Adam Bogdanove ), progress on TALe research has been swift. The incentives are high. Since...potential targetable sites approximately every 35bp. Researchers are still determining the importance of context...matter of days. In an open access Nucleic Acids Research article that came out in the spring of 2011, the...labs that continue to hone these tools and the researchers who are learning to wield them. Learn more about...

...biology data for A. thaliana research. OpenPlant Synthetic Biology Research Centre (Link opens in a new...use in plants. These resources include tools for research using plant systems such as Arabidopsis, wheat...express your gene of interest in plants. You can search the table for features you are looking for, such...Infrastructure Kit Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains...

...Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research Collection The iPSC Neurodegenerative...from this effort will be broadly shared with the research community. To this end, all plasmids that result...the project efforts will be made available for research purposes in the iNDI plasmid collection at Addgene...

...nonprofit research organization with a mission to advance protein science to accelerate research and improve...display technology, these antibodies provide researchers with powerful tools to investigate neurotransmission...detect, or localize particular proteins. Browse or search the table below to find antibodies targeting V5...

...production and transfer plasmids for a wide array of research purposes. Interested in Addgene's viral services...different properties and are suited for specific research goals. For example, AAV vectors are typically ...cellular machinery to generate viral vectors for research and gene therapy. Created with BioRender.com. ...their viral genome. Therefore, viruses used in research typically do not have the ability to propagate...

...Find prime editor plasmids for (search and replace) genome editing....Plant Empty pegRNA Resources Prime editing is a “search and replace” gene editing method in which a reverse...tool from Hyongbum Kim's lab . Browse, sort, or search the tables below for CRISPR prime editing plamisds...editing are highlighted in the table below. Use the search bar to find a gRNA vector based on expression system...

...enables researchers to manipulate the genome with relative ease. Over the past decade, researchers have ...are many different Cas proteins available, and researchers can adapt these experiments for different organisms...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus...proceeding, we recommend asking labmates/colleagues, searching the literature, or discussing with your PI to ...

...recombinant antibodies to help facilitate scientific research. We are building a collection of affinity reagents...Addgene values transparency and accessibility in research and therefore makes the sequences of these affinity...of antibodies developed and validated for many research applications. Browse Antibodies Production Interested...