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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 51 results for: CHI

Showing: 21 - 40 of 51 results

  1. Viral Production

    Type
    Collection
    ...ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method. Titering by the...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...

  2. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... in: A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation... in: Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon ... described in: Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara...

  3. TALEN Plasmids and Kits

    Type
    Collection
    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular tool for scientists... of Renilla luciferase Reuben Shaw 48688 pUltra-Chili-Luc Firefly Ubc Lentiviral expression of firefly... Mammalian expression of firefly luciferase Ming-Chih Lai 100984 pGL4.18 CMV-Luc Firefly CMV Mammalian...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...doxycycline (Zhou et al., 2006). Tet-On 3G systems can achieve >10,000-fold induction of the gene of interest....cell lines or transgenic animals can be made to achieve greater control over expression with flexibility...Galla, M., Maetzig, T., Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral and transposon-based ...

  6. Synthetic Biology - Overview

    Type
    Collection
    ...Resources iGEM - International Genetically Engineered Machine Foundation Registry of Standard Biological Parts...synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource EraSynBio...

  7. Chemogenetics AAV Preps

    Type
    Collection
    ...none 2, 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D(Gq) - Activation HA fusion none 2, 5 Richie 50467 pAAV-CaMKIIa-HA-hM4D...

  8. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013...

  9. Viral Vectors

    Type
    Collection
    ...scientific review of our viral pages, we thank Boston Children's Hospital Viral Core . Viral Plasmid Biosafety...Au S, Panté N. How viruses access the nucleus. Biochim Biophys Acta. 2011 Sep;1813(9):1634-45. doi: 10.1016...

  10. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...using streptavidin affinity purification. For both enCHIP and CAPTURE, design your gRNA sequence to direct...

  11. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible recognition sites flank... for Site-specific Recombination in Drosophila Teaching an Old DOG New Tricks: Controlling Protein Activity...

  12. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...strategy relies on cellular delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double...of PCR products. Finney M, Nisson PE, Rashtchian A. Current Protocols in Molecular Biology. 2001...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ... Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...

  14. Biosensor AAV Preps

    Type
    Collection
    ...Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Patriarchi Oxytocin (OT...

  15. Plan Your Experiment

    Type
    Collection
    ...SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down to location, location...proceeding, we recommend asking labmates/colleagues, searching the literature, or discussing with your PI to ...

  16. TALEN Engineering

    Type
    Collection
    ...assemble TAL effector repeat arrays on a single architectural framework and the DNAs produced by all three...

  18. Mammalian RNAi Tools

    Type
    Collection
    ...a tool in mammalian cell systems, silencing is achieved through the delivery of a double-stranded RNA ...

  19. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine...

  20. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles...
Showing: 21 - 40 of 51 results