... related to cloning and genomic modification, including shuttle, integration, reporter, and tagging vectors... occurring and synthetic regulatory elements, including promoters, terminators, repressors, activators...Addgene's collection of engineered bacterial strains, including plasmid-strain functional pairs. Browse Plasmids...

...humans. Advantages of adenoviruses in research include their large packaging capacity (>8 kb), high titers...the host genome. Disadvantages of adenoviruses include transient transgene expression, as well as the ... in either mammalian cells or microorganisms, including bacteria and yeast. Two plasmids, termed a shuttle...

...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...and several structural and enzymatic proteins, including reverse transcriptase and integrase. Once in a...creation of immortalized cells; additional plasmids include Puro resistance and Neo resistance Weinberg 10676...

...compartments or organelles. Addgene’s database includes many fluorescent biosensors that signal the presence...processes. Note that many of the articles linked below include variants with different binding affinities or negative-control...calcium indicators for optical imaging (GECOs), including blue, green, red, and ratiometric indicators An...biosensors. The ScEnSor Kit from the Olsson laboratory includes eight fluorescent reporters to characterize the...cerevisiae . The MOSAIC Kit from the Cohen laboratory includes 35 plasmids expressing a variety of fluorescent...

...Addgene's collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. ...Traditional sources of antibodies for research purposes include animals or hybridoma cells, but are difficult to...cells, yeast, plants, or insect cells. Benefits include consistency between lots and the ability to optimize...

...for human disease and translational research, including the study of cancer, inflammation and infection...contain tools for use in zebrafish research. This includes plasmids for genome editing, empty backbones, ...bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish...

...cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748 pLKO.3G ...mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619 pLB 3rd Modification...mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730 pLionII 3rd...

... pCS for a variety of systems, including Xenopus HA Epitope Tag pKH3 - N or C-terminal ... 2xHA tag in pCS for a variety of systems, including Xenopus pAG423GPD-ccdB-HA - ... signal For more information about tags, including amino acid sequences, see our list of common...

...curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community ...inactivation, NHEJ, and HDR. Reference genomes include Arabidopsis, C. elegans , sea squirt, cavefish,...common to a set of similar sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish, fly...

... target gene Many different activators exist, including the multi-plasmid SAM system. Select Expression...CRISPR in mammalian cells. Some of the variables include: Species of Cas enzyme and gRNA Species of promoter...verify that your cells contain your desired edit, including but not limited to: Mismatch-cleavage assay (for...

...is not specific to any particular pathogen. It includes physical barriers such as the skin and mucosa,...adaptive immune response is antigen-specific and includes cellular and humoral immunity. Once an adaptive...the surface of antigen presenting cells (APC) including dendritic cells and macrophages. Antigen presentation...through effects on numerous immune cell types including T cells and B cells. It also impacts the development...development and function of innate immune cells including NK cells, macrophages, dendritic cells, and granulocytes...

...TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat...allowing for precise gene editing. These edits can include mutations seen in human patients, protein tags,...off-target editing rates. Advances in this area include: Nickase mutant: cuts only one strand of DNA and...

...which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including DREADDs...Addgene partnership to provide AAV viral preps, includes listing of transfer status for viral preps. Viral...

...related to Networks and Regulation. The collection includes both naturally ocurring and synthetic regulatory...circuits, and higher level gene networks. Examples Include: promoters and terminators repressors and activators...

...also includes the four TALEN expression vectors. More detailed information about REAL, including detailed...

...plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and cytosine...your gene of interest. Available modifications include: histone acetylation by p300 histone demethylation...

...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b....Tools CRISPR Blog Posts Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA...

...See our inventory of chemogenetic constructs, including DREADD constructs....of chemogenetic receptors: Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) and Pharmacologically...

...collection. The plasmids available in this format include a variety of popular plasmids and backbones with...order will need to be completed independently including the check out and will require separate shipping...

...Joung laboratory for engineering TAL effectors, including designed TALENs, and assembly methods for REAL... information about REAL, REAL-Fast and FLASH, including detailed and up-to-date protocols, can be found...