...introduce the various applications of CRISPR, and help you get started using CRISPR in your own research...experiment. There are many online tools available to help you select an optimized gRNA for your application...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved...Synchronizing cells or modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically...or upregulating those in the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins...modified scaffold gRNA and additional RNA-binding helper activators; highest levels of single-gene activation...