... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...2A-Hygro-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse our list of...based on plasmids in our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance...

... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with...Constitutively active Cas9 mouse Available plasmids are described below: 61408 : Targeting vector for the mouse ... backbone plasmid for cloning new targets are described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA...present in the mouse genome. These plasmids are described here. #60227 - AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR...This plasmid enables Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition...plasmid facilitates Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition...

...expressed in the retina). Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization...betaRBPIG Double fluorescent, double selectable cre/loxP reporter Mammalian Hescheler 12463 p231 pCMVe-betaAc-STOP-luc...of interest. Many Cre constructs also contain fluorescent labels that indicate if recombination has occurred... are currently in our repository. ID Plasmid Description Promoter Expression System PI 8394 p209 pCMV-...to regulate your gene of interest: ID Plasmid Description Expression System PI 11578 pSico Cre activates...and making transgenic mice. ID Plasmid/Article Description Expression System PI See article Brainbow constructs...create a stochastic choice of expression among fluorescent proteins, resulting in the indelible marking ...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...have been created by directed evolution of the Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

...catalytically inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9...into a customizable DNA labeler compatible with fluorescence microscopy in living cells. Alternatively, gRNAs...specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic loci. ...live cell imaging. Compared to techniques like fluorescence in situ hybridization (FISH), CRISPR imaging... the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking...

...Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics...and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more.... set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways ...collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology...

...Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical...neuronal activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...

...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used ...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the... to tag endogenous loci with epitope tags or fluorescent markers. Future Directions It’s amazing to see...for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product....

...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning...

... Science has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in... Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids... collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell...plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...

...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

... Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...express plasmid-based antibodies. ID Plasmid Description...

...production of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization... the genomic recognition site. NOTE: Figure 1 describes possible deletion strategies for genes and non-coding...deletion. For this approach, the internal primers described in step 2.1 may be omitted. CRISPR Cloning Anneal...plasmids by electroporation 4 . This protocol is described in detail for murine erythroleukemia (MEL) cells...editing efficiency, but 37 °C is acceptable. Fluorescence Activated Cell Sorting (FACS) of Transfected...

... Users may still generate their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

...3-fold repress on targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR as an...expression cassette, useful for selection or fluorescent gating of transiently transfected cells. Co-expression...can be ordered via the links below: ID Plasmid Description 44246 pdCas9-humanized A catalytically inactive...

...for constitutive shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression...conditional (Cre-lox) shRNA expression ID Plasmid Description PI 11578 pSico Cre addition causes EGFP to be...for Tet-regulated shRNA expression ID Plasmid Description Tet on or off PI 11643 pLVCT-tTR-KRAB 2nd generation...for constitutive shRNA expression ID Plasmid Description PI 8452 pMKO.1 puro Retroviral vector for shRNA...

...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more....Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...

...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains... Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning... and Eric Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the...

...and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI ...vector; for evaluation of shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA...transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast... M, Spangenberg C, Fees S, Fraser S, Eshkind L, Oesch F, Zabel B. Physiol Genomics . 2002 Dec 3;11(3):...

...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...inhibit the target neuron. For more detailed descriptions of chemogenetic receptors, ligands, and how ...preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags PI Do you...