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We narrowed to 45 results for: GFP expression plasmids

Showing: 21 - 40 of 45 results

  1. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ... preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... into a luciferase-containing plasmid and the effects on gene expression can be measured by the luminescence...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging...FLAMP1 and FLAMP2 Chu Lab FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ... of S. cerevisiae MOSAIC Kit includes 35 plasmids expressing a variety of fluorescent sensors to monitor...target RNAs Luciferase Plasmids NanoBRET and NanoBiT reporters in the Promega Plasmid Collection More Resources...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays...

  3. Genetic Code Expansion

    Type
    Collection
    ... you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate...true. Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian...often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place where...different amino acid. They are typically expressed from a single plasmid, with multiple copies of the tRNA. ...Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin... Farren Isaacs Browse Target Plasmids The table below highlights plasmids that contain genes with modified...

  4. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently... can obtain a range of expression levels. This collection comprises 20 plasmids, each carrying two CRISPR...nucleotides. Together, these plasmids allow to explore the whole range of expression levels for these two reporters... modulate expression of two genes without cross‐talk. (A) Schematic of the strain expressing two reporters... reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding...Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols...a collection of CRISPR arrays to modulate the expression levels of two targets independently. The RNA-...

  5. CRISPR Plasmids - Tagging

    Type
    Collection
    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms..., which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG...well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid. Note that the principle...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...requires expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem... sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for the expression of two...

  7. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled... with or without GFP and mCherry controls. Do you have suggestions for other plasmids that should be added... added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve...Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You...

  8. Antibody Plasmid Collection

    Type
    Collection
    ... Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made...created by cloning antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast... Guide ! Cloning and Expressing Antibodies The following table highlights plasmid collections that can...can be used to create and express plasmid-based antibodies. Description Article PI One-step assembly of... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...

  9. Worm Expression Resources

    Type
    Collection
    ... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits...

  10. Antibody Production

    Type
    Collection
    ...mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding...target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target antigen is ... low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified from the supernatant...is first transiently expressed in mammalian cells. To learn which specific QC experiments were performed...tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens...species-specific HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the...

  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References...199419 Anti-GFP [N86/38R-2b] GFP Aequorea victoria Mouse IgG2b 199420 Anti-GFP [N86/8R-2b] GFP Aequorea ...206719 Anti-GFP [N86/38R-1] GFP Aequorea victoria Mouse IgG2a 206720 Anti-GFP [N86/8R-1] GFP Aequorea victoria...Rat Mouse 206743 GFP scFv [N86/44] N86/44 scFv GFP Aequorea victoria Mouse 206744 GFP scFv [N86/20] N86...separate elements of the R-mAb expression plasmid involved in coexpression of light (green) and heavy (blue... Antibody Plasmid Collection NeuroMab Plasmid Collection...into the p1316 plasmid that contains an upstream CMV promoter for light chain expression, and an ER signal...

  12. CRISPR References and Information

    Type
    Collection
    ...vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF,...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide... CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , ... gRNA design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for...lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO ...lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...

  13. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids Other Recombinase Plasmids Recombinase-Dependent...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite site-specific...full catalog for plasmids expressing Cre recombinase and filter the results by expression system and vector...full catalog for plasmids expressing Flp recombinase and filter the results by expression system and vector...full catalog for plasmids expressing Dre recombinase and filter the results by expression system and vector...

  14. Recombinases AAV Preps

    Type
    Collection
    ...which can be used to control gene expression. See our Cre-lox Plasmids page for more information about .../Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP 9, rg* James M. Wilson 105558...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....9, rh10 James M. Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632...Syn EBFP 5 Hongkui Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB James...

  15. Neurodegeneration Research Collection

    Type
    Collection
    ...Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more....CBA-driven, Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al...bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...

  16. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...was developed by Shannon Boye’s lab. Plasmid Information The plasmid below is available in each of the above...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida... preparations were produced with the pTR-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated...above serotypes and buffers. Please click on the plasmid name for ordering information. ID Name Promoter...157970 pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2..., a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

  17. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1...Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent...Packaging Service: Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Schreiter Calcium Sensor...HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Schreiter Dopamine Sensors...

  18. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Vector Guide Find more plasmids in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog...Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector...72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from ...pCDH-EF1-Nluc 73024 Express Nluc from the EF1 promoter pCDH-EF1-copGFP 73030 Expresses copGFP from the EF1 promoter...pCDH-EF1S-Nluc 73033 Expresses Nluc from a truncated EF1 promoter pCDH-EF1s-copGFP 73034 Expressed copGFP from a truncated...pAdx-CMV-copGFP 73346 Expresses copGFP from the CMV promoter pAdx-CMV-tdTomato 73347 Expresses tdTomato...pAdx-EF1-copGFP 73353 Expresses copGFP from the EF1 promoter pAdx-EF1-tdTomato 73354 Expresses tdTomato...

  19. AAV for Neuronal Tracing

    Type
    Collection
    ...direct virus infection specifically into cells that express the TVA viral receptor. Thus, by pseudotyping the...virus with EnvA, only neuronal cells engineered to express TVA will be infected. Schematic depicting steps...to the starter cell. (2) The starter cell now expresses TVA and G-protein. EnvA-pseudotyped RAVB is delivered... via G-protein receptors, which are naturally expressed at presynaptic nerve terminals. (4) Neurons projecting...G-protein coated RABV. These projection neurons do not express G-protein, and thus do not give rise to G-protein...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9-based conditional...
Showing: 21 - 40 of 45 results