...AAV are made from plasmid 37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter...AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin...). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various...37825-AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various...a sample of the purified DNA from the original plasmid that was used to make the virus. For more details...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need.... available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing...

... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging...FLAMP1 and FLAMP2 Chu Lab FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...Kit from the Cohen laboratory includes 35 plasmids expressing a variety of fluorescent sensors to monitor...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other...

...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti...destination plasmid for cDNA expression Ramalho-Santos 24129 pULTRA 3rd bi-cistronic expression of EGFP and the... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis...

...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...as a reference level of expression. Use this table to find plasmids expressing luciferase. We also offer... luciferase expression plasmids; these are noted with the viral service icon . ID Plasmid Luciferase Type...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia...Collections Luciferase Plasmids Luciferase Plasmid Collection You may also like... Fluorescent Proteins Blog...reference level of expression. Scientists have developed a wide range of luciferase-based plasmid systems for...luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016...

... you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate...true. Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian...often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place where...different amino acid. They are typically expressed from a single plasmid, with multiple copies of the tRNA. ... included. Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...

...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently... can obtain a range of expression levels. This collection comprises 20 plasmids, each carrying two CRISPR...nucleotides. Together, these plasmids allow to explore the whole range of expression levels for these two reporters... modulate expression of two genes without cross‐talk. (A) Schematic of the strain expressing two reporters... reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding...Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols...a collection of CRISPR arrays to modulate the expression levels of two targets independently. The RNA-...

...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms..., which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG...well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid. Note that the principle...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...

... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...requires expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem... sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for the expression of two...

... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled... with or without GFP and mCherry controls. Do you have suggestions for other plasmids that should be added... added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve...Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You...

...activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need...to the transient Cas9 expression. The table below summarizes the major expression systems and variables...Delivery method Expression System Components of System Application Mammalian expression vector Cas enzyme... (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA....g. GFP) or selection marker to identify and enrich positive cells Packaging and envelope plasmids provide... or electroporation Transient expression of CRISPR components Expression decreases as RNA is degraded ...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...

...Addgene plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but...pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP and puromycin ...cloning and expression purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene...constructs in which multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide...Cas9 construct (10 μg total). Add 0.5 μg of GFP expression construct. Electroporate cells with 250 volts... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...

... Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made...created by cloning antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast... Guide ! Cloning and Expressing Antibodies The following table highlights plasmid collections that can...can be used to create and express plasmid-based antibodies. Description Article PI One-step assembly of... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...

...high expression, so adding this G could help with expression, though it is possible for the plasmid to ... may also like... Zhang Lab CRISPR plasmids General CRISPR Plasmids Dr. Le Cong, the first author of one...use double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The concept...easiest way is to co-transfect several plasmid together, with each plasmid having a targeting spacer cloned ...you need to have four spacers co-expressed and using PX335 to express the Cas9n (nickase). Homologous ...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang Lab's CRISPR Frequently...

... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits...

...mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding...target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target antigen is ... low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified from the supernatant...is first transiently expressed in mammalian cells. To learn which specific QC experiments were performed...tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens...species-specific HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the...

...BFP fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with...bacterial Cas9 expression plasmid 44250 pwtCas9-bacteria A wild-type Cas9 expression plasmid for use editing... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids Available from... genome. 1. Two-plasmid CRISPRi system for bacterial gene knockdown The first plasmid ( pdCas9_bacteria...resistance. The second plasmid ( pgRNA_bacteria ) contains a constitutive sgRNA expression cassette on a ColE1...arbitrary sequences in the genome. Co-expression of both plasmids in bacteria could cause up to 300-fold...targeted genes. 2. Two-plasmid CRISPRi system for mammalian gene knockdown The first plasmid ( pdCas9_humanized...

...vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF ... CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , ... gRNA design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for...lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF 2.3 MB Zhang GeCKO pooled...lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Kits are also available (...collaboration with the labs who have deposited CRISPR plasmids , we've created a series of links and guides to...on Addgene's blog Protocols Lab(s) Description Plasmids in protocol Download protocol Church gRNA design...

...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References...199419 Anti-GFP [N86/38R-2b] GFP Aequorea victoria Mouse IgG2b 199420 Anti-GFP [N86/8R-2b] GFP Aequorea ...206719 Anti-GFP [N86/38R-1] GFP Aequorea victoria Mouse IgG2a 206720 Anti-GFP [N86/8R-1] GFP Aequorea victoria...Rat Mouse 206743 GFP scFv [N86/44] N86/44 scFv GFP Aequorea victoria Mouse 206744 GFP scFv [N86/20] N86...separate elements of the R-mAb expression plasmid involved in coexpression of light (green) and heavy (blue... Antibody Plasmid Collection NeuroMab Plasmid Collection...into the p1316 plasmid that contains an upstream CMV promoter for light chain expression, and an ER signal...

...fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine with...Cyan Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene of interest...Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal...Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene...Mammalian Express a gene of interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene...Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal...Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover Green Mammalian Expresses Clover...

.../Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP 9, rg* Wilson 105558 pENN.AAV.CamKII...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...recombinases, which can be used to control gene expression. Dre AAV ID Name Promoter Fluorophore Serotype...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....2, 5, 8, 9, rh10 Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre...EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB Wilson...