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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 47 results for: HA tag

Showing: 21 - 40 of 47 results

  1. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase . Each type of luciferase has advantages and disadvantages depending on the application, and ...transcriptional reporters, but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively...Lentiviral expression of firefly luciferase with a V5 tag Kevin Janes 99283 pCW57.1-Luciferase Firefly TRE ...Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (neo)...Lentiviral expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian... : Biosensor using Renilla luciferase and a Halo tag to assay mRNA turnover in real time. Nonsense-mediated...expression Renilla luciferase mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir...

  2. Distribution to Industry

    Type
    Collection
    ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available... it with your organization. If your organization has not ordered from Addgene in the past, we will need...regarding your account verification. Once your account has been verified, you will be able to place your order...our Help Center . The MTA Process: Once your order has been placed on our website, Addgene will email you...behalf of the recipient institution. Once the MTA has been signed, it can be submitted to Addgene through...Information Featured Collections NEW NanoLuc and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2,...

  3. COVID-19 Resources

    Type
    Collection
    ...DETECTR . Feng Zhang's lab at the Broad Institute has utilized the natural RNAse activity of the Cas13 ...UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute has published information and protocols for utilizing...bioRxiv 2020.04.20.052159 . Jennifer Doudna's lab has utilized Cas12a’s non-specific ssDNA degradation ...CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing... out this Suggest a Plasmid form. If the plasmid has been described previously we will reach out to the...Spike Receptor Binding Domain Site-Saturation Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries...

  4. Brain Initiative Collection

    Type
    Collection
    ...Fluorescent reporter for serotonin (dendrite localization tag) Lin Tian 135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used ...support from the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn ...forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan...

  5. Brain Armamentarium

    Type
    Collection
    ...pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting...pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting...pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting...pAAV_BiLAMP5e3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-...

  6. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have suggestions for other plasmids that...products that mediate AAV replication. This requirement has been circumvented with “helper-virus free systems... contaminants of adenovirus stocks. One major advantage of using AAV for research is that it is replication-limited...

  7. Antibody Guide

    Type
    Collection
    ...directly to the sdAb, or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can... immune response to it. Once the immune response has developed, the antibodies are collected, tested against...epitopes on constant regions of other antibodies. This has useful research applications, and is covered in the...exist as monomers. Each antibody class therefore has a valency, or number of binding sites available per...of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description Configuration... must be able to detect if and where an antibody has bound. Signaling molecules , proteins that emit a...to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...expression gene of interest fused with 3xFLAG and 2xStrep-tag from TRE3G promoter for Tandem-Affinity Purification...basic tool to control gene expression, the system has been engineered with a variety of improvements. A...promoter. And, most importantly, the TetR protein has been modified to provide two classes of tet-responsive...TetR and derivatives like rTetR, rtTA, and tTA. Dox has good tissue distribution, low toxicity, a known half-life...operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the TetR protein ... (Tet-On) In 1995, Gossen et al. used random mutagenesis to identify the amino acid residues of TetR that... Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9...

  9. Plasmids for Stem Cell Research

    Type
    Collection
    ...related to embryonic stem cell use and has the major advantage that iPSCs can potentially be derived from... be used to create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection...Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3/4, shp53...dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent...development. Unlike other cell types, each stem cell has the potential to either remain a stem cell or become...can be faster, bypasses intermediate pluripotent stages, and can occur without cell division, all of which...mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology. Browse Addgene's collection...

  10. Bacterial Expression Systems

    Type
    Collection
    ...purification. Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep...bacteria with different purification tag combinations, including PelB tags for localization to the periplasm...Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization To determine...Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, factor..., proteins are often expressed alongside epitope tags, which can also later be used for protein detection...removed using protease cleavage sites. Other protein tags and signal peptides are used to enhance protein ...localization: PelB, MalE, OmpA ID Plasmid Promoter Tags PI Additional Addgene Protein Purification Resources...

  11. CRISPR References and Information

    Type
    Collection
    ...KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA design...PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional HDR...cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49 KB Vosshall and Matthews (Link opens...

  12. Cancer Research Plasmids and Resources

    Type
    Collection
    ...Research Cancer Research Plasmids and Resources Addgene has a large variety of resources for use in cancer signaling...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...

  13. AAV Viral Preps

    Type
    Collection
    ...range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy to use, and can be safely...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti...

  14. Neurodegeneration Research Collection

    Type
    Collection
    ... Neurodegeneration Research Collection Addgene has assembled the following to help you find information...Parkinson's today. The Michael J. Fox Foundation has made a number of tools (Link opens in a new window...sporadic, having no prior family history. A small percentage (5-10%) are familial ALS cases having at least...

  15. Retrograde AAV viral preps

    Type
    Collection
    ...105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn EGFP-tagged Cre expression Recombinases Wilson 105551 pENN....pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP-tagged Cre expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH...Activator DREADD Roth 121539 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) Syn Activator DREADD Richie 50475 pAAV-hSyn-hM4D...See our retrograde AAV based on the functional catagories listed below. Narrow down the items available...

  16. Resolute Plasmid Collection

    Type
    Collection
    ...and pharmaceutical companies. The RESOLUTE project has received funding from the Innovative Medicines Initiative... protein thermal stabilization, and changes of voltage all contribute to assess ligand action/engagement...

  17. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...cytoskeleton or blocking membrane trafficking. Addgene has assembled a collection of fluorescent fusion proteins...protein of interest with a fluorescent protein that has a different emission wavelength. For example, if ...mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-MitoGFP-HA Mitochondria MCU GFP Vamsi Mootha 49153 GFP-Mff Mitochondria-Outer...Voeltz 79802 pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc...Mothes 79806 pTag-RFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-...endosomes Rab11a TagBFP James Johnson 79800 pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP James Johnson...EEA1 GFP Silvia Corvera 42635 TagRFP-T-EEA1 Early endosomes EEA1 TagRFP-T Silvia Corvera 38770 pEF.myc.ER-E2...

  18. Retrovirus Plasmids

    Type
    Collection
    ... expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that...results in long-term transgene expression. Some disadvantages of γ-retroviruses are that they can only transduce... can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide for more information...

  19. AAV for Neuronal Tracing

    Type
    Collection
    ... delivery by an AAV. Since this starter cell now has all the viral genes present, the virus can spread...virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Ian Wickersham Introduction to Rabies...

  20. Caltech Systemic Capsids

    Type
    Collection
    ...used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated...Enhanced CNS tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor... CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA minBetaGlobin SYFP2 Control Ting 163509 CN1839-rAAV-hSyn1-...
Showing: 21 - 40 of 47 results