We narrowed to 44 results for: HA tag

...pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting...pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting...pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting...pAAV_BiLAMP5e3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-...

...to these plasmids, such as different promoters or tags, can even direct delivery of the genetic cargo to...from Addgene's AAV plasmid collection. Addgene also has a selection of in-stock viral preps available. Browse...nm Dividing/Non-dividing No High *Recombinant AAV has a low frequency of target cell genome integration...

...purification. Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep...bacteria with different purification tag combinations, including PelB tags for localization to the periplasm...Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization To determine...Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, factor..., proteins are often expressed alongside epitope tags, which can also later be used for protein detection...removed using protease cleavage sites. Other protein tags and signal peptides are used to enhance protein ...localization: PelB, MalE, OmpA ID Plasmid Promoter Tags PI Additional Addgene Protein Purification Resources...

...83356 pMXs-3XHA-EGFP-OMP25 MoMSV For tagging mitochondria with HA epitopes in mammalian cells. David Sabatini... genome into a host cell. Scientists can take advantage of this to stably integrate transgenes of interest...

...pCINeo-hFX-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV...pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA FXN HA, AU1 T7 Friedreich...pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-HA FXN HA, AU1 T7 Friedreich...-hFX-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 14992 pET28(a)-hFX(I154F)-HA FXN HA, HIS,...AA88)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 15403 pBlue-hFX(I154F)(AA88)-HA FXN HA, HIS...pBlue-hFX-HA(AscI) FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 15405 pBlue-hFX-HA(NcoI/EcoRI) FXN HA, AU1...Camilli 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's Wade Harper 22594 Flag-HA-OTUD4 OTUD4 HA, FLAG CMV Gordon...

...Fluorescent reporter for serotonin (dendrite localization tag) Lin Tian 135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used ...support from the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn ...forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan...

...related to embryonic stem cell use and has the major advantage that iPSCs can potentially be derived from... be used to create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection...Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3/4, shp53...dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent...development. Unlike other cell types, each stem cell has the potential to either remain a stem cell or become...can be faster, bypasses intermediate pluripotent stages, and can occur without cell division, all of which...mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology. Browse Addgene's collection...

...expression gene of interest fused with 3xFLAG and 2xStrep-tag from TRE3G promoter for Tandem-Affinity Purification...basic tool to control gene expression, the system has been engineered with a variety of improvements. A...promoter. And, most importantly, the TetR protein has been modified to provide two classes of tet-responsive... domain than simply removing a repressor. Tet-On has since become one of the most popular methods to induce...TetR and derivatives like rTetR, rtTA, and tTA. Dox has good tissue distribution, low toxicity, a known half-life...operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the TetR protein ... Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9...

...KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA design...PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional HDR...cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49 KB Vosshall and Matthews (Link opens...

...105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn EGFP-tagged Cre expression Recombinases James M. Wilson 105551...pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP-tagged Cre expression Recombinases James M. Wilson 105553...Activator DREADD Bryan Roth 121539 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) Syn Activator DREADD Christopher Richie ...See our retrograde AAV based on the functional catagories listed below. Narrow down the items available...

...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available... it with your organization. If your organization has not ordered from Addgene in the past, we will need...regarding your account verification. Once your account has been verified, you will be able to place your order...our Help Center . The MTA Process: Once your order has been placed on our website, Addgene will email you...behalf of the recipient institution. Once the MTA has been signed, it can be submitted to Addgene through...Information Featured Collections NEW NanoLuc and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2,...

...directly to the sdAb, or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can... immune response to it. Once the immune response has developed, the antibodies are collected, tested against...epitopes on constant regions of other antibodies. This has useful research applications, and is covered in the...exist as monomers. Each antibody class therefore has a valency, or number of binding sites available per...of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description Configuration... must be able to detect if and where an antibody has bound. Signaling molecules , proteins that emit a...to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

...Research Cancer Research Plasmids and Resources Addgene has a large variety of resources for use in cancer signaling...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...

...range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy to use, and can be safely...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti...

...and pharmaceutical companies. The RESOLUTE project has received funding from the Innovative Medicines Initiative... protein thermal stabilization, and changes of voltage all contribute to assess ligand action/engagement...

... remodeling or blocked membrane traffic. Addgene has assembled a collection of popular fluorescent fusion...protein of interest with a fluorescent protein that has a different emission wavelength. For example, if ...mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-MitoGFP-HA Mitochondria MCU EGFP Vamsi Mootha 49153 GFP-Mff ...early) Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagBFP James Johnson...Harrison 79806 pTag-RFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc... Rab11a TagBFP James Johnson 79800 pTag-RFP-C-h-Rab4a-c-Myc Endosomes (recycling) Rab4a TagRFP James Johnson...early) Rab5 TagBFP Gia Voeltz 61802 GFP-Rab5B Endosomes (early) Rab5B AcGFP Gia Voeltz 79802 pTag-RFP-C-h-...

...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...editing systems include targeted AID-mediated mutagenesis (TAM) from the Xing Chang lab and CRISPR-X from...

... delivery by an AAV. Since this starter cell now has all the viral genes present, the virus can spread...virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Ian Wickersham Introduction to Rabies...

...used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated...Enhanced CNS tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor... CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA minBetaGlobin SYFP2 Control Ting 163509 CN1839-rAAV-hSyn1-...

...specific genomic edits, like point mutations or small tag insertions, by copying from a DNA donor template ...contain your desired edit. Each of these methods has their own considerations. Which one to use will come...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral and... if you provided a homology template, a large percentage of DNA breaks will be repaired by NHEJ instead...