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We narrowed to 128 results for: IND

Showing: 21 - 40 of 128 results

  1. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...origins should be used. Find further details in Mohler et al. (2023) and find general tips on phosphoprotein... tRNA^Sep with anticodon, CUA. Engineered EF-Tu binds the phosphoserine-charged tRNA (AUC) and is brought...-terminal portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry fragment... fragment. Upon interaction between the phosphobinding domain and the phosphosite, mCherry fluorescence...phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the WW2 domain of...022-01638-5. PMID 36280721 (Link opens in a new window) rEcoli XpS and a new pSerOTS described in: Mohler...msb.202110591. PMID 37477096 (Link opens in a new window) Protocols for using iSPI, rEcoli XPS, and pSerOTS-C1...

  2. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... in a new window) The Malate Dehydrogenase CUREs Community (MCC) (Link opens in a new window) develops...mentor future MCC members. Find a regional hub (Link opens in a new window) and contact the hub director...opens in a new window) — Learn how to get started with a CURE, explore MDH projects, find teaching resources...expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned... cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present ...plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism or species (e.g., human.... DOI: 10.24918/cs.2021.43 (Link opens in a new window) External Collaboration Results in Student Learning...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...directly after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded...CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in...protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy the...foreign DNA. Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...(tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse III and Cas9...

  4. E11 Bio PRISM Collection

    Type
    Collection
    ... a new window) , in collaboration with The Francis Crick Institute (Link opens in a new window) , MIT ...new window) , and The Max Planck Institute for Biological Intelligence (Link opens in a new window) . ...molecular tools for high-throughput reconstruction of individual neurons in brain tissue developed by scientists...tools enabling high-throughput reconstruction of individual neurons in brain tissue. PRISM begins with the... PRISM is part of an integrated pipeline, but individual components can be used separately for additional...also developed a set of Cre-dependent, hSyn-Tet inducible plasmids (Addgene #242782–242799) suitable for...Potential applications could also include using individual or subsets of barcode proteins to label and detect...

  5. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top Cyan Plasmid...Plasmid Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald Plasmid...Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return to top Orange Plasmid...Plasmid Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab. Plasmid...interest. Most of these plasmids are available to industry scientists as well as academic and non-profit ...Molecular Expressions website (Link opens in a new window) for images, tutorials, microscopy protocols, and...

  6. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell (hiPSC...Telomeric repeat-binding factor 2 (TRF2) Telomeres 168799 CTCF-mEGFP AICSDP-144 mEGFP CCCTC-binding factor (CTCF...in a new window) RNP transfection for gene editing human iPS cells (Link opens in a new window) References... new window) PubMed All Allen Institute for Cell Science Publications (Link opens in a new window)...Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell line information from the Allen ...for Cell Science data portal (Link opens in a new window) , including: data and images from the hiPS cell...Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding protein FUS Paraspeckles/stress granules 114411...

  7. Plant Plasmids and Resources

    Type
    Collection
    ...Plantae (Link opens in a new window) . TraVa (Link opens in a new window) - Database of gene expression...Addgene's plant resources: Find plasmids for use in plants. Plasmid...and a variety of other species. In addition to individual plasmids, Addgene carries several useful toolkits... rice. Includes a protocol (Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang...Biological Resource Center (ABRC) (Link opens in a new window) - The ABRC collects, preserves, reproduces, and...Information Resource (TAIR) (Link opens in a new window) - TAIR includes a database of genetic and molecular...Resource for Plant Biology (Link opens in a new window) - Tools for gene and protein expression and mapping...

  8. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CAG Alzheimer's Dennis Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP ...Aleksandra Radenovic 114194 pc DNA FRT TO- APPSwed/Ind APP CMV Alzheimer's Aleksandra Radenovic 114410 AICSDP...Huntington's Susan Lindquist 1178 p416 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1179 p416 72Q GPD...Huntington's Susan Lindquist 1180 p416 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1181 p426 25Q GPD...Huntington's Susan Lindquist 1182 p426 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1183 p426 72Q GPD...Huntington's Susan Lindquist 1184 p426 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1185 p416 25Q GAL...Huntington's Susan Lindquist 1186 p416 103Q GAL HTT GFP GAL1 Huntington's Susan Lindquist 1187 p426 25Q GAL...

  9. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...38913-z (Link opens in a new window) PMID: 30783202 (Link opens in a new window) Lee, S., Lim, W. A., Thor....0067902 (Link opens in a new window) PMID: 23844123 (Link opens in a new window) Loqué, D., Lalonde, S., ...nature05579 (Link opens in a new window) PMID: 17293878 (Link opens in a new window) Sheff, M. A., Thorn, K. ....1130 (Link opens in a new window) PMID: 15197731 (Link opens in a new window) Vickers, C. E., Bydder, ...12-96 (Link opens in a new window) PMID: 24161108 (Link opens in a new window) Content last reviewed: 12...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...Systems Biology Lab website (Link opens in a new window) References Botman, D., de Groot, D. H., Schmidt...

  10. Microbiology Resources

    Type
    Collection
    ... new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window) National...(Link opens in a new window) NIH AIDS Reagent Program (Link opens in a new window) Saccharomyces Genome...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses... viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with, or...they cause. The standard format for providing individual plasmids is a bacterial stab (of a laboratory...expression, and synthetic biology. CRISPR Resources : Find essential background information on using CRISPR...for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB plasmid set : E. ...

  11. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here....Collections iNDI iPSC Neurodegenerative Disease Initiative (iNDI) Collection You May Also Like... CRISPR ...research purposes in the iNDI plasmid collection at Addgene. Figure 1: Schematic of iNDI iPSC line generation...Plasmid Description Additional Resources iNDI (Link opens in a new window) is an ongoing project of the Center...Collection The iPSC Neurodegenerative Disease Initiative (iNDI) project aims to generate and characterize single...and Related Dementias (CARD) (Link opens in a new window) at the NIH. Reference Ramos et al. 2021. Tackling...:1080-1083. PMID: 33831364 (Link opens in a new window)...

  12. Open Enzyme Collection

    Type
    Collection
    ...NotI (GC-rich recognition site) 165508 pOpen-HindIIIR HindIII 165526 pOpen-PstIR PstI 165528 pOpen-SfiIR...single-stranded DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding Protein 165579... Blog: Golden Gate Cloning (Link opens in a new window) The Open Enzyme Collection consists of more than...protein sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to...functionality. The Open Bioeconomy Lab (Link opens in a new window) designed and codon-optimized the Open Enzymes ...Lab's Open Enzyme Collection (Link opens in a new window) . How to Use The plasmids in the Open Enzyme collection...for all plasmids in this collection is ampicillin. Find more information on the enzymes and protocols for...

  13. Ras Pathway

    Type
    Collection
    ... a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular switches...cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene... proteins with multiple subunits or isoforms, individual links to each gene page are provided below. Color... Color is used for clarity and does not indicate a specific relationship. The content and map for this...Research Color is used for clarity and does not indicate a specific relationship. The content and map for...to top Ras Pathway - Gene List Click on a name to find available plasmids for the gene. For components ...components with multiple subunits or isoforms, individual links to each gene page are provided below. Symbol Name...

  14. Lentivirus Plasmids

    Type
    Collection
    ...versions of this plasmid. Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based) for inducible knockdown,...2nd Envelope Expression of VSV-G glycoprotein (Indiana strain). Use with packaging construct encoding ...selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See ...tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible expression of transgene or shRNA. See article ...Gateway destination plasmid with constitutive or inducible expression of cDNAs, shRNAs or miRNAs, and use...selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression, gateway plasmid, constitutive...

  15. Caltech Systemic Capsids

    Type
    Collection
    ...pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase ...opens in a new window) PHP.V1: Kumar et al., 2020 Pubmed 32313222 (Link opens in a new window) MaCPNS1, MaCPNS2...opens in a new window) AAV9-X1.1: Chen et al., 2023 Pubmed 37291094 (Link opens in a new window) Don’t See... the Caltech CLOVER Center (Link opens in a new window) . As part of our standard viral production , these... the Caltech CLOVER Center (Link opens in a new window) and was adapted from Challis et al. (2022). Text...repository of published capsid use (Link opens in a new window) . Viral Vectors 101: Systemic Capsids PHP.eB These... LY6A ( Huang et al., 2019 (Link opens in a new window) ) to cross the blood brain barrier. For information...

  16. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Calbindin [L109/39R] Calbindin Human Mouse IgG2a 177455 Anti-Calbindin [L109/57R] Calbindin Human...Anti-Neuregulin-HBD (Heparin binding domain, Type I/II) [N120A/9.1R] Neuregulin-HBD (Heparin binding domain, Type I/...Homer1 Mouse Mouse IgG2a 188187 Anti-Calbindin [L109/62R] Calbindin Human Mouse IgG2a 188188 Anti-Homer1... Mouse IgG2b 206635 Anti-CALB1/calbindin [L109/39R-2b] CALB1/calbindin Human Mouse IgG2b 206636 Anti-Homer1L...Human Mouse IgG1 206686 Anti-CALB1/calbindin [L109/39R-1] CALB1/calbindin Human Mouse IgG1 206687 Anti-Homer1L...Co-Rest/RCOR1 Human Mouse IgG2a 222146 Calbindin [L109/43R] Calbindin Human Mouse IgG2a 222147 Homer1 [L113... IgG2a 227042 Calbindin (Homo sapiens) recombinant mouse monoclonal antibody. Calbindin Human Chimera:...

  17. mTOR Pathway

    Type
    Collection
    ...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene...list below. For multiple isoforms or subunits, individual links to each gene page are provided below. Color... Color is used for clarity and does not indicate a specific relationship. The content for this page was...Pathway Color is used for clarity and does not indicate a specific relationship. The content for this ... top mTOR Pathway - Gene List Click on a name to find available plasmids for the gene. For components ...components with multiple isoforms or subunits, individual links to each gene page are provided below. mTORC1 Complex...4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin D1 Also known as CCND1 eIF-4E ...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ... interactions, drug-induced changes, and more. NanoBit (Link opens in a new window) : The NanoLuc® enzyme... of LumiLuc (ATP-independent luciferase derived from teLuc) fused to mScarlet-I. Find more NanoLuc® tools...NanoLuc® is small (19 kDa), very bright, and ATP-independent, and a secreted version of the protein is available...William Kaelin 60495 pSBtet-GP Firefly TRE Dox-inducible expression of firefly luciferase Eric Kowarz 18782...Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc...luciferase reporter for? Browse or use the search box to find a construct that contains your regulatory element... form an active enzyme, generating luminescence. Find plasmids with LgBit and SmBit . Cluc-FKBP and FRB-Nluc...

  19. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...BsaI + HindIII No Caixia Gao 172716 pPEgRNA Bacteria J23119 (BBa_J23119) pegRNA SpeI + HindIII No Tilmann...Find prime editor plasmids for (search and replace) genome editing. CRISPR...gRNA, the pegRNA is designed with a spacer that binds to a specific genomic DNA locus and directs the ... site. The longer pegRNA also encodes a primer binding site (PBS) and the desired edits on an RT template...non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the pegRNA and the desired edit is...optimized RT and/or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance... pegRNA? Try the DeepPrime (Link opens in a new window) design tool from Hyongbum Kim's lab . Browse, ...

  20. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...they adopt an enzymatically active state and can bind and cleave additional RNAs regardless of homology...avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter PI Publication...identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...
Showing: 21 - 40 of 128 results