We narrowed to 80 results for: KIN

...Localization General Cytoskeleton Junctions Membrane Trafficking Additional Resources The subcellular localization... Protein Collection . Return to top Membrane Trafficking The secretory pathway is responsible for delivering...find markers for components of these membrane trafficking pathways and structures. ID Plasmid Structure...Fluorescent Protein Collection . Return to top Looking for more constructs or different fluorescent protein...

...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...Recommended Applications PI Don't See What You're Looking For? Please let us know what you would like to ...

...events and the systemic effects of Cre toxicity. Leakiness Leaky expression, where the gene of interest is...populations. Read our blog post about how to minimize leakiness in experiments using AAV vectors. Highlighted ...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert...

...ncAA) in a protein of interest. Applications By making small changes in selected amino acids within a ...there are several things to consider. If you are working off of a previously established protocol, make ...PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser 140021 ...and References GCE4All Research Center GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...

...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters, but are...a secreted version of the protein is available, making it ideal for a variety of applications. To learn...expressed from a T7 promoter for normalization. John Atkins 174050 pGWB-nLUC Firefly Creation of N-terminal...

...NusA Cleavage sites: TEV protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization...Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent... Engineering Modifying the bacterial genome by knocking out genes or introducing specific mutations can...

...setting up CRISPR experiments. We will focus on making single edits using CRISPR/Cas9 in mammalian cells... the cell type. Before proceeding, we recommend asking labmates/colleagues, searching the literature, ...efficiency. Edit Type The type of edit you are looking for will ultimately depend on which CRISPR method...

...red over time, allowing tracking of processes such as intracellular trafficking or cell cycle dynamics....concentrations, so this is mutation is noted when present. Looking for more empty backbones? Visit our Empty Backbones...

...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...

...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...assistance at [email protected] . Don’t See What You’re Looking For? At this time, Addgene has made a limited number...

... page to help you find what you're looking for. If you are looking for a non-plasmid item, browse our ...

...engineered or identified to increase the spectral and kinetic properties as well as improve the expression and...ChR mCherry", "Cre inhibition", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

...is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA...target DNA using a gRNA and recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling...

...luciferin substrate or by external illumination, making it a bimodal opto-chemogenetic actuator. For more..."KORD AAV", "LMO", "Sternson", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

...Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives support ...

...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny...

...Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas...biological systems for novel functions. Addgene is working with leading synthetic biologists around the world...

...accordance with export laws and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide...Engineering blog post to see how Addgene depositors are making this possible in a range of organisms. Find CRISPR...

... Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin...protocol videos available that may be helpful for working with this plasmid collection. Single cell passaging...