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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 43 results for: POR C

Showing: 21 - 40 of 43 results

  1. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...3rd 4 78,637 Broad GPP Humagne Set C and Humagne Set D 172650 (Set C) 172651 (Set D) Knockout Human Doench...gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources ...pneumoniae M. tuberculosis M. smegmatis Green monkey ( C. sabaeus ) Kaposi's Sarcoma-associated Herpes Virus...collection differ based on some important criteria, and it is important to know which library best...mind: CRISPR screening experiments require electroporation to amplify the pooled library and the use of...best suits your particular experiment. Some important variables include: Type of genetic modification - ...Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina...

  2. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas9 + Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein DR274 42250 C. elegans BsaI none S. pyogenes...gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources ...pyogenes Zhang PX854 62886 Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang pGuide 64711 Mammalian ...pyogenes Zhang PX852 62884 Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang PX855 62887 Mammalian BbsI...pyogenes Zhang PX856 62888 Mammalian BbsI yes, activate, C-terminal S. pyogenes Zhang pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B...Bullock and Port pCFD2-dU6:2gRNA 49409 Fly BbsI none S. pyogenes Virmilion Bullock and Port pDCC6 59985...Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI yes, cut S...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...opens in a new window) Krueger, C., Pfleiderer, K., Hillen, W., & Berens, C. (2004). Tetracycline derivatives...vector to express Tet-On 3G transactivator under the c-Fos promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 ... N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral ...elements like the H1 RNA promoter. And, most importantly, the TetR protein has been modified to provide...identify the amino acid residues of TetR that were important for tetracycline-dependent repression. Mutating...efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet, miniCMV Howard Gu...

  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources ...which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme ...the PBS of the pegRNA and the desired edit is incorporated into the DNA by reverse transcription. The edited...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...and others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish...Zebrafish Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero ...23287718 Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Côté J, Doyon Y. 2015. A Scalable Genome-Editing-Based...Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann ...libraries can be used to identify genes that are important to a given phenotype. Current libraries are available...

  6. p53 Pathway

    Type
    Collection
    ...RFWD2); E3 ubiquitin protein ligase cytoC Cytochrome c DR5 Tumor necrosis factor receptor superfamily, member...Ghebranious N, Igelmann H, Lu X, Soron G, Cooper B, Brayton C, Park SH, Thompson T, Karsenty G, Bradley A, Donehower... of p53’s pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional...

  7. Neurodegeneration Research Collection

    Type
    Collection
    ...aggregation. Saha et al. Nat Commun. 2023 Feb 2. Study the C terminal domain of TDP-43 to better understand its... atlas for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan 7. Use a FRET-based...Collection The BRAIN Initiative supports the development of a diverse portfolio of biomolecular tools and emphasizes...The vast majority of cases (90-95%) of ALS are sporadic, having no prior family history. A small percentage...specific known cause) nature of the disease, a small portion of cases can be attributed to known genetic factors... S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere... (AIS) plasticity with a motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell...

  8. CRISPR Plasmids - Parasites

    Type
    Collection
    ...gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources ...Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania (leishmaniasis), and...

  9. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 177559 Anti-c-Fos [N486/32R] c-Fos Human Mouse IgG2a 177560 Anti-c-Fos [N486/76R] c-Fos Human Mouse...IgG2a 177520 Anti-LRRK2/Dardarin, C-terminus [N241A/34R] LRRK2/Dardarin, C-terminus Human Mouse IgG2a 177521...glutamate receptor Mouse Mouse IgG2a 206619 Anti-c-Fos [N486/25R] c-Fos Human Mouse IgG2a 206620 Anti-Kv1.4 K...220411 LRRK2/Dardarin kinase, C-terminus [N241A/58R] LRRK2/Dardarin kinase, C-terminus Human Mouse IgG2a ...VAPA [N479/111R] VAPA Rat Mouse IgG2a 220414 c-Fos [N486/93R] c-Fos Human Mouse IgG2a 220415 Cavalpha2delta2...] N483/126 Rufy3 Mouse Mouse 182090 C-Fos scFv [N486/32] N486/32 C-Fos Human Mouse 182091 Mortalin/GRP75...84 scFv Rufy3 Mouse Mouse 190560 c-Fos scFv [N486/76] N486/76 scFv c-Fos Human Mouse 190561 BKbeta2 K+...

  10. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...production, including codon optimization, deletion of the C-terminal 18-21 amino acids and a D614G change which...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for... Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing...Plasmid Description Mutations Industry PI Due to export control regulations, MERS and SARS-CoV materials...pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase...destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...

  11. TALEN Engineering

    Type
    Collection
    ...has yielded TALENs which function efficiently in: C. elegans ( Wood et al., Science 2011 ) Zebrafish (...for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL...

  12. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map...research opportunities to their students and other members of the MCC community. They support educators...local hub of educators teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional...

  13. Antibody Guide

    Type
    Collection
    ...stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C in aliquots...conjugated to fluorophores should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate... after thawing and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals due...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating...

  14. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources ...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49409 pCFD2-dU6:2gRNA dU6:2 BbsI Injection or in...transcription Virmilion none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, ...

  15. Synthetic Biology - Overview

    Type
    Collection
    ...Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver...GMP) FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-...Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Science. 2010...Plasmids Joel Kralj Voltage Voltage sensors for use in C. elegans (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...

  17. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski...114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding... from the Allen Institute for Cell Science data portal (Link opens in a new window) , including: data ...

  18. AAVED

    Type
    Collection
    ... Deverman BE, Matho KS, Cetin A, Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J...Sponsor We are grateful to The Kavli Foundation for supporting this meeting. The goal of this year’s workshop...

  19. Zhang Lab CRISPR Page

    Type
    Collection
    ... AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki...nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stemloop...

  20. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry... has developed several tools useful for the incorporation phosphoserine into your gene of interest. Depositor...University changed the way researchers can explore important questions surrounding serine phosphorylation by...in place of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1* ( 188537 ) is a ROP...able to demonstrate unambiguous site-specific incorporation of phosphoserine in >36,000 phosphosites by ...expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...188531 ; 188532 ; 188533 ; 188534 ; 188535 ). Importantly, phosphorylation positional isomers are allocated...
Showing: 21 - 40 of 43 results