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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 56 results for: Set

Showing: 21 - 40 of 56 results

  1. Arf GTPase Family

    Type
    Collection
    ...effector roles). A compatible GEF, GTPase, and GAP set is referred to as a minimal triad, with each component...

  2. TALEN Guide

    Type
    Collection
    ...out in the spring of 2011, the groups describe a set of customized plasmids that can be used with the ...Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN...

  3. Open Enzyme Collection

    Type
    Collection
    ...Vectors Synthetic Biology Plasmids BIOFAB Plasmid Set Drew Endy Lab Plasmids Jennifer Molloy Lab Plasmids...

  4. Ras Pathway

    Type
    Collection
    ... top Resources RAS Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the...

  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... neomycin cassette Zebrafish, Sea urchin, Xenopus, and C. elegans Hamdoun Lab Plasmids - Set includes ...GFP-variants and non-GFP proteins that result in a diverse set of colors. Addgene has assembled a collection of ...mCarmine in pcDNA3 - Mammalian Expression mCarmine in pRSETB - Bacterial Expression mCardinal 604 659 17 5.3...Mammalian Expression pPAGFP-C1 - Mammalian Expression pRSETA-PAGFP - Bacterial Expression tandem PA-GFP-KanR... / 581 47 / 30 5.6/6.4 Prone to oligomerization pRSETa mEos2 - Bacterial Expression pFA6a-link-yomEos2...

  6. CRISPR References and Information

    Type
    Collection
    ...single gene, as well as a target site common to a set of similar sequences. Organisms include human, mouse...cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning in gRNA:...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...Structurally destabilized firefly luciferases : A set of three increasingly destabilized mutants which ...

  8. Bacterial Expression Systems

    Type
    Collection
    ...transcriptional activation or repression. The BIOFAB plasmid set (Addgene #1000000037) contains specific bacterial... and Venus) Escherichia coli Wolf Frommer 20336 pRsetB-his7-Perceval ATP:ADP ratio Fluorescence (GFP) ...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ...Targeted gene activation using SAM There are two sets of 3 vectors each available for mammalian endogenous...2013). We also have an older system using two cassettes to express the CRISPR RNA array and tracrRNA separately...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested.... SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either...This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized SaCas9; SaCas9...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...Hochedlinger MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...

  12. Plan Your Experiment

    Type
    Collection
    ... you started using CRISPR in your research and setting up CRISPR experiments. We will focus on making ...DNA to serve as template for the repair. Once you settle on your gRNA, you can design a donor DNA to have...variety of modifications and will likely contain a subset of wild type cells. If you are screening a variety...

  13. Brain Initiative Collection

    Type
    Collection
    ...research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository....

  14. Antibody Guide

    Type
    Collection
    ...non-biological replicates) often each require their own set of controls. Multiplex Assays Flow cytometry, IF,... cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires...requires single-antibody controls, a small subset of the sample labeled with only one antibody, for each ...

  15. Neurodegeneration Research Collection

    Type
    Collection
    ... early-onset, where symptoms appear between a person’s thirties and mid-sixties, or late-onset, where ...individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It is more likely... results in death within 3-5 years from symptom onset. Disease mechanisms are still poorly understood ...during or after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases...cases of Alzheimer's disease. Of these early-onset forms, a fraction of these are due to a mutation in...such as the ε4 allele, are a risk factor for late-onset AD. Many more genes have been associated with Alzheimer's...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...SLC1A3 CMV Episodic ataxia Susan Amara 32868 SETD1A SETD1A His T7 Parkinson's Cheryl Arrowsmith 32880 FLAG-MATR3...Parkinsonism, early-onset Roger Reeves 53822 SYNJ1-170_pCSdest SYNJ1 CMV, SP6 Parkinsonism, early-onset Roger Reeves...ALS Catherine Tomasetto 104450 pEGFPC1-hVAP-B KD/MD VAPB GFP, HA CMV ALS Catherine Tomasetto 104465 hnRNPA2...SERPINI1 Halo Dementia Michael Ward 178169 SETX_Halo_C_allele SETX Halo ALS, Ataxia with neuropathy Michael...C6A/C111A SOD1 ALS Nam-Chul Ha 193056 pADC10-SETXhel SETX Flag CMV, p10 ALS, Ataxia with neuropathy Andrew...infantile onset Smita Patel 205056 SUMO-Twinkle 43-684 TWNK His Spinocerebellar ataxia, infantile onset Smita...disease Kivanc Birsoy 218838 3xFlag-eGFP-Flag-SETX SETX Flag, GFP CMV ALS, Ataxia with neuropathy Albert...

  17. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... an exogenous donor cassette, the donor cassette replaces the target site cassette in a double-crossover... sites (often called a "lox-STOP-lox" or "LSL" cassette) placed upstream of a gene of interest will prevent...-layered genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Rolf Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

  18. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate ...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369...gRNA module vectors, multiple gRNA expression cassettes can be assembled using Golden Gate cloning or ...polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into a Cas9 expressing vector. Yang...to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR...sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC...

  19. Lentivirus Plasmids

    Type
    Collection
    ...Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730... plasmid with a chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned ...interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). See...

  20. CRISPR Plasmids - Tagging

    Type
    Collection
    ...plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins. The ... The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and gRNA...PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL). To ...cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for... expression vector allows for efficient marker cassette FLP-out. Plasmids can be found associated with...
Showing: 21 - 40 of 56 results