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We narrowed to 78 results for: TRI

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Showing: 21 - 40 of 78 results

  1. Viral Production

    Type
    Collection
    ...subjected to quality control by Addgene before being distributed to customers. Details about our production protocols...control are described below. AAV Production AAV distributed by Addgene has been produced either in-house ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone...

  2. Lentivirus Plasmids

    Type
    Collection
    ...selection. See plasmid 21916 for neomycin selection. Dmitri Wiederschain 11578 pSico 3rd Conditional (Cre-lox...shRNA. See Szulc et al. (2006) for more variants. Patrick Aebischer and Didier Trono 11795 pLL3.7 3rd Expresses...selection. See plasmid 21915 for puromycin selection. Dmitri Wiederschain 36084 pLV-mCherry 3rd CMV driven expression...expressing envelope plasmid. Arthur Nienhuis and Patrick Salmon 17532 pLTR-G Expression of VSV-G glycoprotein...VSV-G-expressing envelope plasmid. Arthur Nienhuis and Patrick Salmon 22501 pHEF-VSVG VSV-G-expressing envelope...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Dyche Mullins 41147 pEGFP Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 ...41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170 EGFP Erich Nigg 41163 pEGFP PICH Centromeres...EB1 EGFP Ken-Ichi Takemaru 122871 GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru 54234...-alpha Tubulin Microtubules alpha-Tubulin EGFP Patricia Wadsworth 27123 eTC GFP beta-actin full length...

  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrioles 101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-...lines have been deposited here with Addgene for distribution to the research community. With these plasmids...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...

  5. AAV Packaged on Request

    Type
    Collection
    ...amplification Viral vector production Density centrifugation purification Addgene’s comprehensive quality... and results through Addgene’s Data Hub . By contributing your AAV vector experience, from methods to ...titration, and quality control measures. We have distributed preps around the globe and shared our well-developed...lab, and even managing legal permissions and distribution. Troubleshooting If your answer isn’t in the...

  6. p53 Pathway

    Type
    Collection
    ...candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin...stresses like DNA damage, oxidative stress, and nutrient deprivation. p53 is activated through both phosphorylation...ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E ...

  7. Open Enzyme Collection

    Type
    Collection
    ...Enzyme Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are useful in both basic...the links below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert ...

  8. Fluorescent Proteins: FRET

    Type
    Collection
    ...N1 , mVenus N1 mTFP1 mCitrine 462 0.85 529 94,000 0.74 5.9 2.6 mTFP1-N1 , mCitrine-pBAD EGFP mCherry 488...pNCS-CyOFP , mCardinal-N1 mAmetrine* tdTomato 406 0.58 581 138,000 0.69 6.5 7.1 mAmetrine-N1 , tdTomato-N1 mTurquoise2...

  9. COVID-19 Resources

    Type
    Collection
    ... this effort by providing a platform for the distribution of plasmid-based tools that are useful for COVID... a for-profit entity, please see our Plasmid Distribution to Industry page. ID Plasmid Description Industry...Collection – Addgene partnered with Ginkgo Bioworks to distribute SARS-CoV-2 plasmids for E. coli , yeast, and ... plasmids - a cysteine endosomal protease that triggers proteolysis of SARS-CoV Spike protein, activating...

  10. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S...Spiny Neurons Lentiviral Human Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion.... 2017 Aug;9(8):1117-1131. Parmar Fibroblasts Dentritic Cells Lentiviral Mouse Direct reprogramming of...

  11. CRISPR Guide

    Type
    Collection
    ...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... Acidaminococcus sp. ). The popularity can be attributed to Cas12a’s many benefits. Cas12a exhibits increased... quantification and can be used on lateral flow strips. Other Cas enzymes can be added to SHERLOCKv2 to...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... (12), 770–788. PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....152 (5), 1173–1183. PMID: 23452860 Savic, D., Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows...

  12. AAV Viral Preps

    Type
    Collection
    ... viral vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality...genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified viral particles...Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and...

  13. Retrovirus Plasmids

    Type
    Collection
    ...and Patrick Salmon 35616 pCAG-VSVG VSV-G-expressing envelope plasmid. Arthur Nienhuis and Patrick Salmon...35614 pBS-CMV-gagpol Contains MoMLV gag and pol . Patrick Salmon Return to top Popular Gamma-Retroviral Envelope...

  14. Biosensor AAV Preps

    Type
    Collection
    ...Tommaso Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Tommaso Patriarchi Oxytocin...pAAV-Ef1a-fDIO-GCaMP6f EF1a GCaMP6f none Flp dependent 1 Patricia Jensen 135632 pAAV-S5E2-GCaMP6f E2 GCaMP6f none...pAAV-Ef1a-fDIO-JRGECO1a EF1a jRGECO1a none Flp dependent 1 Patricia Jensen Calcium Sensor: sRGECO 137125 pAAV-Ef1a-sRGECO...

  15. Microbiology Resources

    Type
    Collection
    ...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...Addgene continues to monitor its library and distributions in accordance with export laws and biosafety...Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. ...

  16. Neurodegeneration Research Collection

    Type
    Collection
    ...caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT) gene. An individual... the disease, a small portion of cases can be attributed to known genetic factors such as defects in LRRK2...organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein regions with FUS and HNRNPA1...

  17. Deisseroth INTRSECT Collection

    Type
    Collection
    ...131779 pAAV-nEF-Con/Fon TVA-mCherry Cre AND Flp No Triple recombinase-dependent Addgene ID Plasmid Logic ... evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786... Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron...

  18. Tetracycline Inducible Expression

    Type
    Collection
    ...like rTetR, rtTA, and tTA. Dox has good tissue distribution, low toxicity, a known half-life (24 hours),...Plasmid #21916 for neomycin selection. TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral ...Tet-Off and additional plasmids. tetR-KRAB TRE Patrick Aebischer , Didier Trono 41393 pCW57.1 Lentiviral...

  19. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Michael Davidson from Florida State University has contributed their comprehensive collection of ORFs tagged...found below. In addition, the Davidson lab has contributed the corresponding empty backbone with different...

  20. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...plasmids available to scientists in academic and industrial organizations. For more information on ordering... a for-profit entity, please see our Plasmid Distribution to Industry page. These plasmids have not been...
Showing: 21 - 40 of 78 results