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We narrowed to 38 results for: cas9 plasmid

Showing: 21 - 38 of 38 results
  1. CRISPR Plasmids - Single-Strand Break (Nick)

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    Collection
    ...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR... CRISPR Nick CRISPR Plasmids: Nick Mammalian...homology-directed repair (HDR). Figure 1: Overview of Cas9 nickase cleavage and repair. Browse, sort, or search...search the tables below for CRISPR nickase plasmids. To learn more about CRISPR nickases and other CRISPR...
  2. CRISPR Plasmids - RNA Targeting

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    Collection
    ...to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top Plant ID Plasmid Gene/Insert PI Publication...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Mammalian Bacteria Plant Resources Type VI CRISPR systems...crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require that each DNA target have...Browse, sort, or search the tables below for CRISPR plasmids designed for RNA targeting. To learn more about...
  3. CRISPR Plasmids - Purify Genomic Loci

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    Collection
    ...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Browse, sort, or search the tables below for CRISPR plasmids designed for the purification of genomic loci....CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...
  4. CRISPR Plasmids for Genomic Visualization

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    Collection
    ...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR... CRISPR Visualize CRISPR Plasmids: Visualize...Browse, sort, or search the tables below for CRISPR plasmids designed for the visualization of genomic loci...CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...
  5. CRISPR Plasmids - Double-Strand Break (Cut)

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    Collection
    ...following cleavage by Cas9. Browse, sort, or search the tables below for CRISPR plasmids designed to introduce...Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert...elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that create double strand breaks in DNA. CRISPR...
  6. Botman-Teusink Yeast FP Collection

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    Collection
    ...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials Kurt Thorn...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs ...proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are...codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter...promoter and the URA3 marker, the p415 plasmid (Loqué et al., 2007) containing the TEF1 promoter and the LEU2...
  7. Distribution to Industry

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    Collection
    ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering ...type, species, and more. Plasmids Browse the table below to see all of the plasmids that are currently available...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity...Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts ...
  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome...of your cells will take up your plasmid. Thus, many plasmids have markers on them so that you...yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection...
  9. Allen Institute for Cell Science Plasmid Collection

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    Collection
    ...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...
  10. Cre-Lox and Other Site-Specific Recombinases

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    Collection
    ...Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids Other Recombinase Plasmids Recombinase-Dependent... vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout... Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources ...Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Return to top Other Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter...
  11. Rett Syndrome

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    Collection
    ...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...
  12. Bacterial Expression Systems

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    Collection
    ...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...Expansion Collection Microbiology Plasmids Collection Synthetic Biology Plasmids Collection Empty Backbones ...under the microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent...New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events and molecules...like CRISPR-Cas9 are not only valuable but often essential. Find a collection of CRISPR plasmids that have...Guide . Browse CRISPR plasmids available at Addgene through our CRISPR Plasmids and Resources page. Check...
  13. Tetracycline Inducible Expression

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    Collection
    ...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters... for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma Rawlins ...
  14. Bikard Lab - CRISPR Repression Collection

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    Collection
    ...enzymes. The pLC97 plasmid allows for quick integration of the aTc-inducible pTet-dCas9 in the attachment...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols...expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first one...18, or 20 matching nucleotides. Together, these plasmids allow to explore the whole range of expression... sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds...high‐throughput microscopy for a collection of 20 CRISPR plasmids. Each point represents a single cell, and each...
  15. CRISPR Plasmids and Resources

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    Collection
    ...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...variety of factors. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed for use in bacteria.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in...
  16. CRISPR Plasmids - Activate Gene Expression

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    Collection
    ...Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert...C. elegans ID Plasmid Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid that ...need to use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Figure...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...
  17. CRISPR Plasmids - Bacteria

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    Collection
    ...following CRISPR plasmids have been designed for use in bacteria. To find emtpy gRNA plasmids, visit Addgene's...Activate ID Plasmid Gene/Insert Promoter PI Publication Return to top Base Edit ID Plasmid Promoter PI... top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert...top Nick ID Plasmid Gene/Insert Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Purify ID Plasmid Gene/Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-...
  18. Neurodegeneration Plasmid Collection

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    Collection
    ...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...others. The table below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent...particular gene of interest, please search our full plasmid collection using the search bar at the top of this...
Showing: 21 - 38 of 38 results