We narrowed to 88 results for: cript

...: dCas9 fused to transcriptional activators or repressors can modulate transcription in a reversible manner...are available for gene knockout, as well as transcriptional activation or repression. Combined with the... fused to epigenetic modifiers can modulate transcriptions and create heritable epigenetic marks. Visualize...CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell . 154(2):442-51. PMID: 23849981...

...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

... for gRNA expression . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty...Scott McComb 145025 TFORF3549 3rd For barcoding transcription factors for identification in pooled screens...Lentiviral Packaging Plasmids ID Plasmid Generation Description PI 1732 pCPRDEnv 2nd Part of the FELIX vector...Popular Lentiviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

... CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived growth factor 3, pseudogene CR-3, CRIPTO, CRIPTO...nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription factor...RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L - RELB v-rel reticuloendotheliosis...beta CBF-A, CBF-B, HAP3, NF-YB NFYC nuclear transcription factor Y, gamma CBF-C, CBFC, DKFZp667G242, FLJ45775..., PA28G, REG-GAMMA RAET1E retinoic acid early transcript 1E LETAL, MGC125308, MGC125309, RAET1E2, ULBP4...CCL15 chemokine ligand 14, chemokine ligand 15 transcription unit - CCL15 chemokine (C-C motif) ligand 15...

...allows you to fuse proteins of interest to transcriptional regulatory elements that will turn on gene ...selection of plasmids using tetracycline-regulated transcriptional activation or repression. The BIOFAB plasmid...that element. When this activation requires a transcription factor to bind to a small molecule, the reporter...DNA-binding specificity of proteins such as transcription factors. Addgene Blog Luciferase Technologies...

...KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse roles in processes ...elements or GC-rich regions on DNA to mediate transcriptional activation and/or repression. Continuing collaborative...Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...

...CRISPR plasmids for transcriptional repression of target genes....Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. This interference is most efficient when the gRNA...

...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific...transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused to an activator..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

...for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production of viruses containing...adenoviral vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use ... for adenoviral vector production. ID Strain Description PI 16398 BJ5183 E. coli strain for recombination...

...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...neutralizing activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral ...provirus and the overall strength of viral transcriptional response in these cells. Warner Greene 105621...

... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance...2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...

... packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging...for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid,...repository for AAV vector production. ID Plasmid Description PI Return to top Other Resources Addgene's Viral...

... on Request for many more. Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded...Yan Qin Return to top Small Molecules Target Description Article PI Acetyl-CoA PancACe acetyl-CoA sensor...Frommer Return to top Neurotransmitters Target Description Article PI Acetylcholine Family of ACh sensors...Clapham Return to top Cell Physiology Target Description Article PI Autophagy Measure autophagic flux ...caspase", "mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible for Viral Packaging...

...specificity phosphatase E2F E2F1 E2F2 E2F3 E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR...protein kinase ETS ETS1 ETS2 ETS proto-oncogene, transcription factor EXOC EXOC1 EXOC2 EXOC3 EXOC4 EXOC5 EXOC6...TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2 Transcription factor Dp TIAM TIAM1 TIAM2 T-cell lymphoma invasion...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat...targeting vector that encodes both a guide RNA transcript and a repair template for an individual tagging...

...started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene...and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9...105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression...

...references are below. Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR...) is an engineered protein complex for the transcriptional activation of endogenous genes. It consists... to GeCKO Library SAM Library Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to... plasmids from this depositor's lab. Plasmid Description Vector Type Selectable Marker PI Publication ...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...

...opto-chemogenetic actuator. For more detailed descriptions of chemogenetic receptors, ligands, and how ...preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for...