...CRISPR plasmids for transcriptional repression of target genes....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression...

...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain (TRD) the TRD contains a...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...

... the REAL-Fast assembly method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

...reverse transcriptase and integrase. Once in a target cell, the virus uses the reverse transcriptase in tow...envelope and packaging plasmids. ID Plasmid Type Description PI 8449 pUMVC Packaging Contains MoMLV gag and...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that increases export to...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Mammalian U6x2 yes, nick S. pyogenes Puro Jackson pBluescriptSKII+ U6-sgRNA(F+E) empty 74707 Mammalian hU6 none...from our collection. Plasmids Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit ...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...

...124606 SOX2-mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964...159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression...

... Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...express plasmid-based antibodies. ID Plasmid Description...

...example, search "Cdc42", "transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector ...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...

...: dCas9 fused to transcriptional activators or repressors can modulate transcription in a reversible manner...are available for gene knockout, as well as transcriptional activation or repression. Combined with the... fused to epigenetic modifiers can modulate transcriptions and create heritable epigenetic marks. Visualize...CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell . 154(2):442-51. PMID: 23849981...

... CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived growth factor 3, pseudogene CR-3, CRIPTO, CRIPTO...nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription factor...RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L - RELB v-rel reticuloendotheliosis...beta CBF-A, CBF-B, HAP3, NF-YB NFYC nuclear transcription factor Y, gamma CBF-C, CBFC, DKFZp667G242, FLJ45775..., PA28G, REG-GAMMA RAET1E retinoic acid early transcript 1E LETAL, MGC125308, MGC125309, RAET1E2, ULBP4...CCL15 chemokine ligand 14, chemokine ligand 15 transcription unit - CCL15 chemokine (C-C motif) ligand 15...

...Access to the Paper & Protocol Video Download the manuscript here: Bauer et al, 2014 322.2 KB If you have ...referenced Tables and Figures, please access the manuscript PDF posted at the top of the page. CRISPR Design...leading to nonsense mediated decay of the mRNA transcript ( Figure 1B ). Use the example guides for the...representative results and discussion, download the manuscript here: Bauer et al, 2014 322.2 KB Genome engineering...

...KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse roles in processes ...elements or GC-rich regions on DNA to mediate transcriptional activation and/or repression. Continuing collaborative...Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...

... are currently in our repository. ID Plasmid Description Promoter Expression System PI 8394 p209 pCMV-... Mammalian Luo 47117 pT3TS-Cre for in vitro transcription of Cre lac Bacterial Ekker 47551 pDD104 (Peft...mCherry-p2A-CreERT2-FRT-kan-FRT For in vitro transcription of Cre or to recombine into BAC SP6 Bacterial...to regulate your gene of interest: ID Plasmid Description Expression System PI 11578 pSico Cre activates...and making transgenic mice. ID Plasmid/Article Description Expression System PI See article Brainbow constructs...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250...42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other...

...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific...transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused to an activator..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

...strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination between... plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral For recombining... be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain...

... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance...2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...

...with the viral service icon . Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded...Yan Qin Return to Top Small Molecules Target Description Article PI Acetyl-CoA PancACe acetyl-CoA sensor...Frommer Return to Top Neurotransmitters Target Description Article PI Acetylcholine Family of ACh sensors...Clapham Return to Top Cell Physiology Target Description Article PI Autophagy Measure autophagic flux ...in Living Cells", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type PI Publication Return...