Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 68 results for: fus

Showing: 21 - 40 of 68 results

  1. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9...cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor...have been made by optimizing expression of the fusions, modifying the linker region between Cas variant...deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase...CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). ...

  2. CRISPR Plasmids - Plants

    Type
    Collection
    ... Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB.... pyogenes Yang 47024 pUC gRNA Shuttle Mt U6.6 inFusion none S. pyogenes Parrott 50591 pCBC-DT2T3 see ...

  3. Brain Initiative Collection

    Type
    Collection
    ...AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal...expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation Viviana Gradinaru...

  4. Fluorescent Protein Guide

    Type
    Collection
    ... Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When... and/or function of your protein of interest by fusing it to a fluorescent protein. FRET Find FRET pairs...resides by using a well-characterized fluorescent fusion protein. In Vivo Imaging Image with these powerful...FRET based biosensors - Pertz Fluorescent Protein Fusion Construct Set - Hamdoun Browse All Plasmid Kits...

  5. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Uncx Mouse Mouse IgG2a 114547 Anti-Mitofusin-2 [N171/17.4R] Mitofusin-2 Mouse Mouse IgG2a 114548 Anti-Mortalin...SNAPAP Human Mouse IgG2a 140072 Anti-Mitofusin-1 [N111/24R] Mitofusin-1 Mouse Mouse IgG2a 140073 Anti-Pannexin...Mouse IgG2a 225377 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a...IgG2a 225378 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a.../Tmem90b Rat Mouse 211603 Mitofusin-1 scFv [N111/24] N111/24 scFv Mitofusin-1 Rat Mouse 211604 Botch scFv...Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising a κ light chain...heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed by their insertion...

  6. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...transactivator (tTA) was developed. tTA was created by fusing tetR with the C-terminal domain of VP16 (virion...etracycline-controlled t rans a ctivator) was created by fusing rTetR with VP16. The tetracycline on system is ...CMV-driven; linked via IRES to EGFP-Blasticidin fusion; pMA2641 has rtTA driven by retroviral LTR promoters...than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression...

  8. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation...mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes...

  9. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions; Coding Prefix alleviates RBS-CDS issues. Prohibited...6 bp scar encodes Ala-Ser; Allows for in-frame fusions; NheI is rare in E. Coli genome. Prohibited Restriction... 10 w/ start and stop codons; Creates in-frame fusions w/ linker Thr-Gly; Use N-parts prefix to preserve...

  10. CRISPR Plasmids - Yeast

    Type
    Collection
    ... Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...

  11. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the...expressing and purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...

  12. Lentivirus Plasmids

    Type
    Collection
    ...variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance... of pULTRA. Moore 19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd...pLV-mCherry 3rd CMV driven expression of mCherry fusion. See plasmids 36083 and 36086 for other FP variants...

  13. COVID-19 Resources

    Type
    Collection
    ...Coronavirus Class I Fusion Protein Upstream of Rather than Adjacent to the Fusion Peptide . TACE - Heurich...SARS-CoV Spike protein, activating its membrane fusion function.( 5 ) TACE - (ADAM17) a metalloprotease...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ... RNA (sgRNA), which is derived from a synthetic fusion of the CRISPR RNA (crRNA) and the trans-activating...components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers at the...This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return...

  15. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs).... Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates...

  16. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Davidson lab fluorescent protein fusion constructs and backbones....with different fluorescent tags for you to create fusion proteins with your gene of interest. Please visit...

  17. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...Posts Using catalytically inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have...microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers, which recruit...

  18. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or gRNA for efficient purification. Various...antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing...

  19. Recombinases AAV Preps

    Type
    Collection
    ... pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Deisseroth 55636 pAAV-EF1a-Cre EF1a...pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a fusion tag) 1, rg* Deisseroth 55637 pAAV-EF1a-Flpo EF1a...

  20. Retrovirus Plasmids

    Type
    Collection
    ...Tetracycline inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN...rapid loss of dsRed and the activation of your gene fused to eGFP expression Clevers 18760 MSCV IRES Luciferase...
Showing: 21 - 40 of 68 results