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We narrowed to 38 results for: grna design

Showing: 21 - 38 of 38 results

  1. CRISPR Guide

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    ...optimized gRNA for your application (see: gRNA Design Software ). In addition to an optimized gRNA design... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide... between the gRNA scaffold and surface-exposed, positively-charged grooves on Cas9. gRNA binding induces...

  2. CRISPR Plasmids - dCas9-FokI

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    ...Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive... break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9...

  3. CRISPR Plasmids - Single-Strand Break (Nick)

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    ... . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of ...enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close...

  4. CRISPR Plasmids - Base Edit

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    ... . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within...protein (Mu- GAM). While many base editors are designed to work in a very narrow window proximal to the...

  5. CRISPR Plasmids - RNA Editing

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    ... . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...or search the tables below for CRISPR plasmids designed for RNA editing. To learn more about RNA editing...

  6. CRISPR Plasmids - Cascade-Cas3

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    ... . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. After nicking the non-targeted...

  7. CRISPR Plasmids - Repress Gene Expression

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    ... . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...transcription. This interference is most efficient when the gRNA targets your gene of interest’s promoter, enhancer...

  8. CRISPR Plasmids - Epigenetics

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    ... . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...or search the tables below for CRISPR plasmids designed for epigenetic modification. To learn more about...

  9. CRISPR Plasmids - RNA Targeting

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    ... . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...or search the tables below for CRISPR plasmids designed for RNA targeting. To learn more about RNA targeting...

  10. CRISPR History and Development for Genome Engineering

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    ...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution... 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target genomic locus...is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed ...it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are...very low off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...

  11. Zhang Lab CRISPR Page

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    ...may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids...oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed based on...expression of an sgRNA targeting the neuronal-specific gene NeuN. As a control they designed an sgRNA targeting... oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold...scarlessly into the vector before the sgRNA scaffold. The oligos are designed based on the target site sequence...phosphorylated oligos designed based on the target site sequence (20bp). #60230 - AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...

  12. Lentivirus Plasmids

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    ...to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid...Tet-inducible lentivirus for ORF expression, multicistronic design enables tracking of viral transduction. See plasmid... based) for inducible knockdown, multicistronic design enables tracking of viral transduction. See Meerbrey...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ...

  15. Microbiology Resources

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    ...CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR resources Bacterial...recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK : Assembly ...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ... Gateway compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors...needed for replication in the host cell and are designed for a user to insert a gene of interest. They ...start codon for N-terminal tags. And when you are designing your plasmid you should keep your gene "in...Plasmids and Resources collection, and browse our Empty gRNA Expression Vectors page Cre-lox Site-specific recombination...selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral...- Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral...Zeocin®/Bleo Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops...

  17. Zebrafish Plasmid Collection

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    ... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic zebrafish...

  18. Tetracycline Inducible Expression

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    ...promoters (sometimes generally called pTet) have been designed by combining tet O sequences with elements like...as much flexibility and control as a dual vector design, but it is easier to implement and ensures that...Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression, with puromycin selection...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...
Showing: 21 - 38 of 38 results